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01-11-2012 | Head and Neck Oncology

Intraoperative Molecular Assessment for Lymph Node Metastasis in Head and Neck Squamous Cell Carcinoma Using One-Step Nucleic Acid Amplification (OSNA) Assay

Authors: Takashi Matsuzuka, MD, PhD, Katsumasa Takahashi, MD, PhD, Daisuke Kawakita, MD, PhD, Naoyuki Kohno, MD, PhD, Hiroshi Nagafuji, MD, Koichi Yamauchi, MD, PhD, Masahiro Suzuki, MD, Tomohiro Miura, MD, PhD, Nobuhiko Furuya, MD, PhD, Yasushi Yatabe, MD, PhD, Keitaro Matsuo, MD, PhD, MSc, Koichi Omori, MD, PhD, Yasuhisa Hasegawa, MD, PhD

Published in: Annals of Surgical Oncology | Issue 12/2012

Abstract

Background

Conventional intraoperative pathological examination for Sentinel node navigation surgery (SNNS) has been controversial. We evaluated the efficacy of one-step nucleic acid amplification (OSNA) assay for intraoperative diagnosis of cervical lymph node (CLN) metastasis compared with histopathological examination in patients with head and neck squamous cell carcinoma (HNSCC).

Methods

A total of 175 CLNs dissected from 56 patients with HNSCC who underwent surgery at Aichi Cancer Center, Kyorin University, Gunma University or Fukushima Medical University, between April 2008 and December 2011 were enrolled. CLN samples were sectioned into four equal pieces, with two of each used for OSNA assay and other histopathological examinations. The diagnostic value of OSNA assay in HNSCC patients in predicting the results of histopathological diagnosis was evaluated using the area under the receiver operating characteristic (AUROC) curve.

Results

OSNA assay showed acceptable efficacy in the detection of pathological CLN metastasis (AUROC 0.918, 95 % confidence interval [CI] 0.852–0.984). Regarding the CK19mRNA cutoff value, the optimum cutoff point in HNSCC patients was 131 copies/μl (sensitivity: 82.4, 95 % CI 65.5–93.2; specificity: 99.3, 95 % CI 96.1–100.0; positive likelihood ratio 116.1; negative likelihood ratio 0.2].

Conclusions

We demonstrated that OSNA assay is useful in intraoperative diagnosis for CLN metastasis in patients with HNSCC. OSNA assay could be applied for SNNS in HNSCC patients.

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Title: Intraoperative Molecular Assessment for Lymph Node Metastasis in Head and Neck Squamous Cell Carcinoma Using One-Step Nucleic Acid Amplification (OSNA) Assay
Authors: Takashi Matsuzuka, MD, PhD
Katsumasa Takahashi, MD, PhD
Daisuke Kawakita, MD, PhD
Naoyuki Kohno, MD, PhD
Hiroshi Nagafuji, MD
Koichi Yamauchi, MD, PhD
Masahiro Suzuki, MD
Tomohiro Miura, MD, PhD
Nobuhiko Furuya, MD, PhD
Yasushi Yatabe, MD, PhD
Keitaro Matsuo, MD, PhD, MSc
Koichi Omori, MD, PhD
Yasuhisa Hasegawa, MD, PhD
Publication date: 01-11-2012
Publisher: Springer-Verlag
Published in: Annals of Surgical Oncology / Issue 12/2012
Print ISSN: 1068-9265
Electronic ISSN: 1534-4681
DOI: https://doi.org/10.1245/s10434-012-2409-0

At a glance: The STEP trials

Springer Medicine

Intraoperative Molecular Assessment for Lymph Node Metastasis in Head and Neck Squamous Cell Carcinoma Using One-Step Nucleic Acid Amplification (OSNA) Assay

Abstract

Background

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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