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Annals of Surgical Oncology
Published in: Annals of Surgical Oncology 8/2011

01-08-2011 | Gastrointestinal Oncology

One-Step Nucleic Acid Amplification (OSNA) for the Application of Sentinel Node Concept in Gastric Cancer

Authors: Yoshihisa Yaguchi, MD, Hidekazu Sugasawa, MD, PhD, Hironori Tsujimoto, MD, PhD, Hideki Takata, PhD, Kadzuki Nakabayashi, Takashi Ichikura, MD, PhD, Satoshi Ono, MD, PhD, Shuichi Hiraki, MD, PhD, Naoko Sakamoto, MD, PhD, Takuya Horio, MD, PhD, Isao Kumano, MD, Yasuhiro Otomo, PhD, Hidetaka Mochizuki, MD, PhD, Junji Yamamoto, MD, PhD, Kazuo Hase, MD, PhD

Published in: Annals of Surgical Oncology | Issue 8/2011

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Abstract

Purpose

This study was designed to apply safely the sentinel node navigation surgery (SNNS) to the malignancies, an accurate and prompt intraoperative diagnosis of SN is essential, and micrometastasis has been frequently missed by conventional frozen sections. Recently, a novel molecular-based rapid diagnosis for the lymph node (LN) metastases has been developed using (OSNA) in breast cancer, which takes approximately 30 min to obtain a final result. We evaluated the efficacy of OSNA in terms of the intraoperative diagnosis of LN metastasis in patients with gastric cancer.

Methods

A total of 162 LNs dissected from 32 patients with gastric cancer was included in this study; 45 LNs were pathologically diagnosed as metastatic LNs and 117 LNs were negative. The LNs were bisected; halves were examined with H&E stain, and the opposite halves were subjected to OSNA analyses of CK19 mRNA. The CK19 mRNA expression was examined in the positive or negative metastatic LNs, and the correlation between the tumor volume and CK19 mRNA expression in the metastatic LNs was examined.

Results

The CK19 mRNA expressions in the positive metastatic LNs were significantly higher than those of negative LNs. When 250 copies/μl was set as a cutoff value, the concordance rate was 94.4%, the sensitivity was 88.9%, and the specificity was 96.6%. The OSNA expression was significantly correlated with the estimated tumor volumes in the metastatic LNs.

Conclusions

The OSNA method is feasible and acceptable for detecting LN metastases in patients with gastric cancer. This should be applied for the intraoperative diagnosis in the SN-navigation surgery in gastric cancer.
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Metadata
Title
One-Step Nucleic Acid Amplification (OSNA) for the Application of Sentinel Node Concept in Gastric Cancer
Authors
Yoshihisa Yaguchi, MD
Hidekazu Sugasawa, MD, PhD
Hironori Tsujimoto, MD, PhD
Hideki Takata, PhD
Kadzuki Nakabayashi
Takashi Ichikura, MD, PhD
Satoshi Ono, MD, PhD
Shuichi Hiraki, MD, PhD
Naoko Sakamoto, MD, PhD
Takuya Horio, MD, PhD
Isao Kumano, MD
Yasuhiro Otomo, PhD
Hidetaka Mochizuki, MD, PhD
Junji Yamamoto, MD, PhD
Kazuo Hase, MD, PhD
Publication date
01-08-2011
Publisher
Springer-Verlag
Published in
Annals of Surgical Oncology / Issue 8/2011
Print ISSN: 1068-9265
Electronic ISSN: 1534-4681
DOI
https://doi.org/10.1245/s10434-011-1591-9

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