Top

Maxillofacial Plastic and Reconstructive Surgery

Published in:

Open Access 01-12-2016 | Research

Effect of hydroxyapatite on critical-sized defect

Authors: Ryoe-Woon Kim, Ji-Hyoung Kim, Seong-Yong Moon

Published in: Maxillofacial Plastic and Reconstructive Surgery | Issue 1/2016

Abstract

Background

Xenologous or synthetic graft materials are commonly used as an alternative for autografts for guided bone regeneration. The purpose of this study was to evaluate effectiveness of carbonate apatite on the critical-size bone defect of rat’s calvarium.

Methods

Thirty-six critical-size defects were created on 18 adult male Sprague-Dawley rat calvaria under general anesthesia. Calvarial bones were grinded with 8 mm in daimeter bilaterally and then filled with (1) no grafts (control, n = 10 defects), (2) bovine bone mineral (Bio-Oss®, Geistlich Pharma Ag. Swiss, n = 11 defects), and (3) hydroxyapatite (Bongros®, Bio@ Inc., Seongnam, Korea, n = 15 defects). At 4 and 8 weeks after surgery, the rats were sacrificed and all samples were processed for histological and histomorphometric analysis.

Results

At 4 weeks after surgery, group 3 (42.90 ± 9.33 %) showed a significant difference (p < 0.05) compared to the control (30.50 ± 6.05 %) and group 2 (28.53 ± 8.62 %). At 8 weeks after surgery, group 1 (50.21 ± 6.23 %), group 2 (54.12 ± 10.54 %), and group 3 (50.92 ± 6.05 %) showed no significant difference in the new bone formation.

Conclusions

Bongros®-HA was thought to be the available material for regenerating the new bone formation.

Buser D, Hoffmann B, Bernard JP et al (1998) Evaluation of filling materials in membrane‐protected bone defects. A comparative histomorphometric study in the mandible of miniature pigs. Clin Oral Implants Res 9:137–150. doi:10.1034/j.1600-0501.1998.090301.x CrossRefPubMed

Burchardt H (1987) Biology of bone transplantation. Orthop Clin North Am 18:187–196PubMed

KURZ LT, GARFIN SR, BOOTH REJ (1989) Harvesting autogenous iliac bone grafts: a review of complications and techniques. Spine 14:1324CrossRefPubMed

Suh H (2000) Tissue restoration, tissue engineering and regenerative medicine. Yonsei Medical Journal 41(6):681–684.CrossRefPubMed

Hollinger JO, Kleinschmidt JC (1990) The critical size defect as an experimental model to test bone repair materials. J Craniofac Surg 1:60–68CrossRefPubMed

Schmitz JP, Hollinger JO (1986) The critical size defect as an experimental model for craniomandibulofacial nonunions. Clin Orthop Relat Res 205:299PubMed

Bosch C, Melsen B, Vargervik K (1998) Importance of the critical-size bone defect in testing bone-regenerating materials. J Craniofac Surg 9:310–316CrossRefPubMed

Pryor ME, Polimeni G, Koo K-T et al (2005) Analysis of rat calvaria defects implanted with a platelet-rich plasma preparation: histologic and histometric observations. J Clin Periodontol 32:966–972. doi:10.1111/j.1600-051X.2005.00772.x CrossRefPubMed

Termine JD, Eanes ED, Greenfield DJ et al (1973) Hydrazine-deproteinated bone mineral. Physical and chemical properties. Calcif Tissue Res 12:73–90CrossRefPubMed

10.

Legeros RZ, Trautz OR, Legeros JP et al (1967) Apatite crystallites: effects of carbonate on morphology. Science 155:1409–1411. doi:10.1126/science.155.3768.1409 CrossRefPubMed

11.

Uchida A, Araki N, Shinto Y et al (1990) The use of calcium hydroxyapatite ceramic in bone tumour surgery. J Bone Joint Surg (Br) 72:298–302

12.

Daculsi G, Laboux O, Malard O, Weiss P (2003) Current state of the art of biphasic calcium phosphate bioceramics. J Mater Sci Mater Med 14:195–200CrossRefPubMed

13.

Mustoe TA, Pierce GF, Thomason A et al (1987) Accelerated healing of incisional wounds in rats induced by transforming growth factor-beta. Science 237:1333–1336. doi:10.1126/science.2442813 CrossRefPubMed

14.

Brown GL, Curtsinger LJ, White M et al (1988) Acceleration of tensile strength of incisions treated with EGF and TGF-beta. Ann Surg 208:788–794CrossRefPubMedPubMedCentral

15.

Handschel J, Wiesmann HP, Stratmann U et al (2002) TCP is hardly resorbed and not osteoconductive in a non-loading calvarial model. Biomaterials 23:1689–1695CrossRefPubMed

16.

Slotte C, Lundgren D (1999) Augmentation of calvarial tissue using non-permeable silicone domes and bovine bone mineral. An experimental study in the rat. Clin Oral Implants Res 10:468–476CrossRefPubMed

17.

Donos N, Lang NP, Karoussis IK et al (2004) Effect of GBR in combination with deproteinized bovine bone mineral and/or enamel matrix proteins on the healing of critical‐size defects. Clin Oral Implants Res 15:101–111. doi:10.1111/j.1600-0501.2004.00986.x CrossRefPubMed

18.

Park J-W, Bae S-R, Suh J-Y et al (2008) Evaluation of bone healing with eggshell-derived bone graft substitutes in rat calvaria: a pilot study. J Biomed Mater Res A 87:203–214. doi:10.1002/jbm.a.31768 CrossRefPubMed

19.

Bigi A, Cojazzi G, Panzavolta S et al (1997) Chemical and structural characterization of the mineral phase from cortical and trabecular bone. J Inorg Biochem 68:45–51CrossRefPubMed

20.

Gibson IR, Bonfield W (2002) Novel synthesis and characterization of an AB-type carbonate-substituted hydroxyapatite. J Biomed Mater Res 59:697–708CrossRefPubMed

21.

Nelson DG, Featherstone JD (1982) Preparation, analysis, and characterization of carbonated apatites. Calcif Tissue Int 34(Suppl 2):S69–S81PubMed

22.

Hoexter DL (2002) Bone regeneration graft materials. J Oral Implantol 28:290–294. doi:10.1563/1548-1336(2002)028<0290:BRGM>2.3.CO;2 CrossRefPubMed

23.

Schwartz Z, Goldstein M, Raviv E et al (2007) Clinical evaluation of demineralized bone allograft in a hyaluronic acid carrier for sinus lift augmentation in humans: a computed tomography and histomorphometric study. Clin Oral Implants Res 18:204–211. doi:10.1111/j.1600-0501.2006.01303.x CrossRefPubMed

24.

Woodard JR, Hilldore AJ, Lan SK et al (2007) The mechanical properties and osteoconductivity of hydroxyapatite bone scaffolds with multi-scale porosity. Biomaterials 28:45–54. doi:10.1016/j.biomaterials.2006.08.021 CrossRefPubMed

25.

Hsu YH, Turner IG, Miles AW (2007) Mechanical characterization of dense calcium phosphate bioceramics with interconnected porosity. J Mater Sci Mater Med 18:2319–2329. doi:10.1007/s10856-007-3136-0 CrossRefPubMed

26.

Tencer AF, Woodard PL, Swenson J, Brown KL (1988) Mechanical and bone ingrowth properties of a polymer-coated, porous, synthetic, coralline hydroxyapatite bone-graft material. Ann N Y Acad Sci 523:157–172CrossRefPubMed

27.

Jensen SS, Broggini N, Weibrich G et al (2005) Bone regeneration in standardized bone defects with autografts or bone substitutes in combination with platelet concentrate: a histologic and histomorphometric study in the mandibles of minipigs. Int J Oral Maxillofac Implants 20:703–712PubMed

28.

Valentini P, Abensur D, Densari D et al (1998) Histological evaluation of Bio-Oss in a 2-stage sinus floor elevation and implantation procedure. A human case report. Clin Oral Implants Res 9:59–64CrossRefPubMed

29.

Kim DK, Cho TH, Song YM et al (2007) A study about early osteoconductivity of porous alloplastic carbonapatite and anorganic bovine xenograft in canine maixlliary augmentation model. J Korean Assoc Maxillofac Plast Reconstr Surg 29:485–493

Title: Effect of hydroxyapatite on critical-sized defect
Authors: Ryoe-Woon Kim
Ji-Hyoung Kim
Seong-Yong Moon
Publication date: 01-12-2016
Publisher: Springer Berlin Heidelberg
Published in: Maxillofacial Plastic and Reconstructive Surgery / Issue 1/2016
Electronic ISSN: 2288-8586
DOI: https://doi.org/10.1186/s40902-016-0072-2

At a glance: The STEP trials

Springer Medicine

Effect of hydroxyapatite on critical-sized defect

Abstract

Background

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

Please log in to get access to this content

Other articles of this Issue 1/2016

Clinical evaluation of sinus bone graft in patients with mucous retention cyst

Radiographic study of the distribution of maxillary intraosseous vascular canal in Koreans

Three-dimensional morphometric analysis of mandibule in coronal plane after bimaxillary rotational surgery

Frontal augmentation as an adjunct to orthognathic or facial contouring surgery

Retrospective study on factors affecting the prognosis in oral cancer patients who underwent surgical treatment only

Simultaneous gap arthroplasty and intraoral distraction and secondary contouring surgery for unilateral temporomandibular joint ankylosis