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International Journal of Implant Dentistry
Published in: International Journal of Implant Dentistry 1/2017

Open Access 01-12-2017 | Research

The influence of surface texture and wettability on initial bacterial adhesion on titanium and zirconium oxide dental implants

Authors: Torsten Wassmann, Stefan Kreis, Michael Behr, Ralf Buergers

Published in: International Journal of Implant Dentistry | Issue 1/2017

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Abstract

Background

This study aims to investigate bacterial adhesion on different titanium and ceramic implant surfaces, to correlate these findings with surface roughness and surface hydrophobicity, and to define the predominant factor for bacterial adhesion for each material.

Methods

Zirconia and titanium specimens with different surface textures and wettability (5.0 mm in diameter, 1.0 mm in height) were prepared. Surface roughness was measured by perthometer (R a ) and atomic force microscopy, and hydrophobicity according to contact angles by computerized image analysis. Bacterial suspensions of Streptococcus sanguinis and Staphylococcus epidermidis were incubated for 2 h at 37 °C with ten test specimens for each material group and quantified with fluorescence dye CytoX-Violet and an automated multi-detection reader.

Results

Variations in surface roughness (R a ) did not lead to any differences in adhering S. epidermidis, but higher R a resulted in increased S. sanguinis adhesion. In contrast, higher bacterial adhesion was observed on hydrophobic surfaces than on hydrophilic surfaces for S. epidermidis but not for S. sanguinis. The potential to adhere S. sanguinis was significantly higher on ceramic surfaces than on titanium surfaces; no such preference could be found for S. epidermidis.

Conclusions

Both surface roughness and wettability may influence the adhesion properties of bacteria on biomaterials; in this context, the predominant factor is dependent on the bacterial species. Wettability was the predominant factor for S. epidermidis and surface texture for S. sanguinis. Zirconia did not show any lower bacterial colonization potential than titanium. Arithmetical mean roughness values R a (measured by stylus profilometer) are inadequate for describing surface roughness with regard to its potential influence on microbial adhesion.
Literature
1.
Poon CY, Bhushan B. Comparison of surface roughness measurements by stylus profiler, AFM and non-contact profiler. Wear. 1995;190:76–88.CrossRef
2.
Hahnel S, Rosentritt M, Handel G, Bürgers R. Surface characterization of dental ceramics and initial streptococcal adhesion in vitro. Dent Mater. 2009;25:969–75.CrossRefPubMed
3.
Abrahamsson I, Berglundh T, Lindhe J. Soft tissue response to plaque formation at different implant systems. A comparative study in the dog. Clin Oral Implants Res. 1998;9:73–9.CrossRefPubMed
4.
Scarano A, Piattelli M, Caputi S, Favero GA, Piattelli A. Bacterial adhesion on commercially pure titanium and zirconium oxide disks: an in vivo human study. J Periodontol. 2004;75:292–6.CrossRefPubMed
5.
Elter C, Heuer W, Demling A, Hannig M, Heidenblut T, Bach FW, et al. Supra- and subgingival biofilm formation on implant abutments with different surface characteristics. Int J Oral Maxillofac Implants. 2008;23:327–34.PubMed
6.
Oh TJ, Yoon J, Misch CE, Wang HL. The causes of early implant bone loss: myth or science? J Periodontol. 2002;73:322–33.CrossRefPubMed
7.
Zitzmann NU, Abrahamsson I, Berglundh T, Lindhe J. Soft tissue reactions to plaque formation at implant abutments with different surface topography. An experimental study in dogs. J Clin Periodontol. 2002;29:456–61.CrossRefPubMed
8.
Busscher HJ, Rinastiti M, Siswomihardjo W, van der Mei HC. Biofilm formation on dental restorative and implant materials. J Dent Res. 2010;89:657–65.CrossRefPubMed
9.
Van Brakel R, Cune MS, Van Winkelhoff AJ, De Putter C, Verhoeven JW, Van Der Reijden W. Early bacterial colonization and soft tissue health around zirconia and titanium abutments: an in vivo study in man. Clin Oral Implants Res. 2010. doi:10.​1111/​j.​1600-0501.​2010.​02005.PubMed
10.
Al-Ahmad A, Wiedmann-Al-Ahmad M, Faust J, Bachle M, Follo M, Wolkewitz M, et al. Biofilm formation and composition on different implant materials in vivo. J Biomed Mater Res B Appl Biomater. 2010;95:101–9.CrossRefPubMed
11.
Teughels W, Van Assche N, Sliepen I, Quirynen M. Effect of material characteristics and/or surface topography on biofilm development. Clin Oral Implants Res. 2006;17:68–81.CrossRefPubMed
12.
Quirynen M, Bollen CM. The influence of surface roughness and surface-free energy on supra- and subgingival plaque formation in man. A review of the literature. J Clin Periodontol. 1995;22:1–14.CrossRefPubMed
13.
An YH, Friedman RJ. Concise review of mechanisms of bacterial adhesion to biomaterial surfaces. J Biomed Mater Res. 1998;43:338–48.CrossRefPubMed
14.
Palmquist A, Omar OM, Esposito M, Lausmaa J, Thomsen P. Titanium oral implants: surface characteristics, interface biology and clinical outcome. J R Soc Interface. 2010;7:515–27.CrossRef
15.
Hannig C, Hannig M. The oral cavity—a key system to understand substratum-dependent bioadhesion on solid surfaces in man. Clin Oral Investig. 2009;13:123–39.CrossRefPubMed
16.
Kawai K, Urano M. Adherence of plaque components to different restorative materials. Oper Dent. 2001;26:396–400.PubMed
17.
18.
Rimondini L, Cerroni L, Carrassi A, Torricelli P. Bacterial colonization of zirconia ceramic surfaces: an in vitro and in vivo study. Int J Oral Maxillofac Implants. 2002;17:793–8.PubMed
19.
Poortinga AT, Bos R, Busscher HJ. Measurement of charge transfer during bacterial adhesion to an indium tin oxide surface in a parallel plate flow chamber. J Microbiol Methods. 1999;38:183–9.CrossRefPubMed
20.
Lima EM, Koo H, Vacca Smith AM, Rosalen PL, Del Bel Cury AA. Adsorption of salivary and serum proteins and bacterial adherence on titanium and zirconia ceramic surfaces. Clin Oral Implants Res. 2008;19:780–5.CrossRefPubMed
21.
Tanner A, Maiden MF, Lee K, Shulman LB, Weber HP. Dental implant infections. Clin Infect Dis. 1997;25:213–7.CrossRef
22.
Bundy KJ, Butler MF, Hochman RF. An investigation of the bacteriostatic properties of pure metals. J Biomed Mater Res. 1980;14:653–63.CrossRefPubMed
23.
Leonhardt A, Dahlen G. Effect of titanium on selected oral bacterial species in vitro. Eur J Oral Sci. 1995;103:382–7.CrossRefPubMed
24.
Rimondini L, Fare S, Brambilla E, Felloni A, Consonni C, Brossa F, et al. The effect of surface roughness on early in vivo plaque colonization on titanium. J Periodontol. 1997;68:556–62.CrossRefPubMed
25.
Bürgers R, Gerlach T, Hahnel S, Schwarz F, Handel G, Gosau M. In vivo and in vitro biofilm formation on two different titanium implant surfaces. Clin Oral Implants Res. 2010;21:156–64.CrossRefPubMed
26.
Burgers R, Hahnel S, Reichert TE, Rosentritt M, Behr M, Gerlach T, et al. Adhesion of Candida albicans to various dental implant surfaces and the influence of salivary pellicle proteins. Acta Biomater. 2010;6:2307–13.CrossRefPubMed
27.
Quirynen M, Bollen CM, Papaioannou W, Van Eldere J, van Steenberghe D. The influence of titanium abutment surface roughness on plaque accumulation and gingivitis: short-term observations. Int J Oral Maxillofac Implants. 1996;11:169–78.PubMed
28.
Hannig M. Transmission electron microscopy of early plaque formation on dental materials in vivo. Eur J Oral Sci. 1999;107:55–64.CrossRefPubMed
29.
Quirynen M, van der Mei HC, Bollen CM, Schotte A, Marechal M, Doornbusch GI. An in vivo study of the influence of the surface roughness of implants on the microbiology of supra- and subgingival plaque. J Dent Res. 1993;72:1304–9.CrossRefPubMed
30.
Nyvad B, Fejerskov O. Scanning electron microscopy of early microbial colonization of human enamel and root surfaces in vivo. Scand J Dent Res. 1987;95:287–96.PubMed
31.
Gatewood RR, Cobb CM, Killoy WJ. Microbial colonization on natural tooth structure compared with smooth and plasma-sprayed dental implant surfaces. Clin Oral Implants Res. 1993;4:53–64.CrossRefPubMed
32.
Wennerberg A, Sennerby L, Kultje C, Lekholm U. Some soft tissue characteristics at implant abutments with different surface topography. A study in humans. J Clin Periodontol. 2003;30:88–94.CrossRefPubMed
33.
Grossner-Schreiber B, Teichmann J, Hannig M, Dorfer C, Wenderoth DF, Ott SJ. Modified implant surfaces show different biofilm compositions under in vivo conditions. Clin Oral Implants Res. 2009;20:817–26.CrossRefPubMed
34.
Rupp F, Scheideler L, Rehbein D, Axmann D, Geis-Gerstorfer J. Roughness induced dynamic changes of wettability of acid etched titanium implant modifications. Biomaterials. 2004;25:1429–38.CrossRefPubMed
35.
Quirynen M, Bollen CM, Willems G, van Steenberghe D. Comparison of surface characteristics of six commercially pure titanium abutments. Int J Oral Maxillofac Implants. 1994;9:71–6.PubMed
36.
Albrektsson T, Wennerberg A. Oral implant surfaces: part 1—review focusing on topographic and chemical properties of different surfaces and in vivo responses to them. Int J Prosthodont. 2004;17:536–43.PubMed
37.
Bollen CM, Papaioanno W, Van Eldere J, Schepers E, Quirynen M, van Steenberghe D. The influence of abutment surface roughness on plaque accumulation and peri-implant mucositis. Clin Oral Implants Res. 1996;7:201–11.CrossRefPubMed
38.
Quirynen M, De Soete M, van Steenberghe D. Infectious risks for oral implants: a review of the literature. Clin Oral Implants Res. 2002;13:1–19.CrossRefPubMed
39.
Weerkamp AH, Uyen HM, Busscher HJ. Effect of zeta potential and surface energy on bacterial adhesion to uncoated and saliva-coated human enamel and dentin. J Dent Res. 1988;67:1483–7.CrossRefPubMed
40.
Barbour ME, O’Sullivan DJ, Jenkinson HF, Jagger DC. The effects of polishing methods on surface morphology, roughness and bacterial colonisation of titanium abutments. J Mater Sci Mater Med. 2007;18:1439–47.CrossRefPubMed
41.
Taylor RL, Verran J, Lees GC, Ward AJ. The influence of substratum topography on bacterial adhesion to polymethyl methacrylate. J Mater Sci Mater Med. 1998;9:17–22.CrossRefPubMed
42.
Quirynen M, Van der Mei HC, Bollen CM, Van den Bossche LH, Doornbusch GI, van Steenberghe D, et al. The influence of surface-free energy on supra- and subgingival plaque microbiology. An in vivo study on implants. J Periodontol. 1994;65:162–7.CrossRefPubMed
43.
Subramani K, Jung RE, Molenberg A, Hammerle CH. Biofilm on dental implants: a review of the literature. Int J Oral Maxillofac Implants. 2009;24:616–26.PubMed
44.
Grivet M, Morrier JJ, Benay G, Barsotti O. Effect of hydrophobicity on in vitro streptococcal adhesion to dental alloys. J Mater Sci Mater Med. 2000;11:637–42.CrossRefPubMed
45.
Bürgers R, Schneider-Brachert W, Rosentritt M, Handel G, Hahnel S. Candida albicans adhesion to composite resin materials. Clin Oral Investig. 2009;13:293–9.CrossRefPubMed
46.
Nassar U, Meyer AE, Ogle RE, Baier RE. The effect of restorative and prosthetic materials on dental plaque. Periodontol. 2000;1995:8,114–124.
47.
Mabboux F, Ponsonnet L, Morrier JJ, Jaffrezic N, Barsotti O. Surface free energy and bacterial retention to saliva-coated dental implant materials—an in vitro study. Colloids Surf B Biointerfaces. 2004;25:199–205.CrossRef
48.
Weerkamp AH, van der Mei HC, Busscher HJ. The surface free energy of oral streptococci after being coated with saliva and its relation to adhesion in the mouth. J Dent Res. 1985;64:1204–10.CrossRefPubMed
49.
Verran J, Taylor RL, Lees GC. Bacterial adhesion to inert thermoplastic surfaces. J Mater Sci Mater Med. 1996;7:597.CrossRef
50.
Drake DR, Paul J, Keller JC. Primary bacterial adhesion of implant surfaces. Int J Oral Maxillofac Implants. 1999;14:226–32.PubMed
51.
Lim YJ, Oshida Y. Initial contact angle measurements on variously treated dental/medical titanium materials. Biomed Mater Eng. 2001;11:325–41.PubMed
52.
Steinberg D, Sela MN, Klinger A, Kohavi D. Adhesion of periodontal bacteria to titanium and titanium alloy powders. Clin Oral Implants Res. 1998;9:67–72.CrossRefPubMed
53.
Bakker DP, Postmus BR, Busscher HJ, van der Mei HC. Bacterial strains isolated from different niches can exhibit different patterns of adhesion to substrata. Appl Environ Microbiol. 2004;70:3758–60.CrossRefPubMedPubMedCentral
54.
Carlen A, Rudiger SG, Loggner I, Olsson J. Bacteria-binding plasma proteins in pellicles formed on hydroxyapatite in vitro and on teeth in vivo. Oral Microbiol Immunol. 2003;18:203–7.CrossRefPubMed
55.
Edgerton M, Lo SE, Scannapieco FA. Experimental salivary pellicles formed on titanium surfaces mediate adhesion of streptococci. Int J Oral Maxillofac Implants. 1996;11:443–9.PubMed
56.
Grossner-Schreiber B, Griepentrog M, Haustein I, Muller WD, Lange KP, Briedigkeit H, et al. Plaque formation on surface modified dental implants. An in vitro study. Clin Oral Implants Res. 2001;12:543–51.CrossRefPubMed
57.
58.
Kolenbrander PE, Ganeshkumar N, Cassels FJ, Hughes CV. Coaggregation: specific adherence among human oral plaque bacteria. FASEB J. 1993;7:406–13.PubMed
59.
Del Curto B, Brunella MF, Giordano C, Pedeferri MP, Valtulina V, Visai L, et al. Decreased bacterial adhesion to surface-treated titanium. Int J Artif Organs. 2005;28:718–30.PubMed
60.
Freedman AM, Jackson IT. Infections in breast implants. Infect Dis Clin North Am. 1989;3:275–87.PubMed
Metadata
Title
The influence of surface texture and wettability on initial bacterial adhesion on titanium and zirconium oxide dental implants
Authors
Torsten Wassmann
Stefan Kreis
Michael Behr
Ralf Buergers
Publication date
01-12-2017
Publisher
Springer Berlin Heidelberg
Published in
International Journal of Implant Dentistry / Issue 1/2017
Electronic ISSN: 2198-4034
DOI
https://doi.org/10.1186/s40729-017-0093-3

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