Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress 2023 EHA Congress 2023 ASCO Annual Meeting
Clinical Collections
Advances in Alzheimer’s

At a glance: The ONWARDS insulin icodec trials

A round-up of the ONWARDS phase 3 clinical trials evaluating the novel weekly insulin icodec in people with diabetes.
ADVANCED SEARCH
Log in
Top
International Journal of Implant Dentistry
Published in: International Journal of Implant Dentistry 1/2017

Open Access 01-12-2017 | Research

Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up

Authors: P. W. Kämmerer, D. G. E. Thiem, A. Alshihri, G. H. Wittstock, R. Bader, B. Al-Nawas, M. O. Klein

Published in: International Journal of Implant Dentistry | Issue 1/2017

Login to get access

Abstract

Background

Mechanostimuli of different cells can affect a wide array of cellular and inter-cellular biological processes responsible for dental implant healing. The purpose of this in vitro study was to establish a new test model to create a reproducible flow-induced fluid shear stress (FSS) of osteoblast cells on implant surfaces.

Methods

As FSS effects on osteoblasts are detectable at 10 dyn/cm2, a custom-made flow chamber was created. Computer-aided verification of circulation processes was performed. In order to verify FSS effects, cells were analysed via light and fluorescence microscopy.

Results

Utilising computer-aided simulations, the underside of the upper plate was considered to have optimal conditions for cell culturing. At this site, a flow-induced orientation of osteoblast cell clusters and an altered cell morphology with cellular elongation and alteration of actin fibres in the fluid flow direction was detected.

Conclusions

FSS simulation using this novel flow chamber might mimic the peri-implant situation in the phase of loaded implant healing. With this FSS flow chamber, osteoblast cells’ sensitivity to FSS was verified in the form of morphological changes and cell re-clustering towards the direction of the flow. Different shear forces can be created simultaneously in a single experiment.
Appendix
Available only for authorised users
Literature
1.
Ehrlich PJ, Lanyon LE. Mechanical strain and bone cell function: a review. Osteoporos Int. 2002;13(9):688–700.CrossRefPubMed
2.
Vaughan TJ, Haugh MG, Mcnamara LM. A fluid-structure interaction model to characterize bone cell stimulation in parallel-plate flow chamber systems. J R Soc Interface. 2013;10(81):20120900.CrossRefPubMedPubMedCentral
3.
Weinbaum S, Cowin SC, Zeng Y. A model for the excitation of osteocytes by mechanical loading-induced bone fluid shear stresses. J Biomech. 1994;27(3):339–60.CrossRefPubMed
4.
Anderson EJ, Falls TD, Sorkin AM, Knothe Tate ML. The imperative for controlled mechanical stresses in unraveling cellular mechanisms of mechanotransduction. Biomed Eng Online. 2006;5:27.CrossRefPubMedPubMedCentral
5.
Bancroft GN, Sikavitsas VI, van den Dolder J, Sheffield TL, Ambrose CG, Jansen JA, et al. Fluid flow increases mineralized matrix deposition in 3D perfusion culture of marrow stromal osteoblasts in a dose-dependent manner. Proc Natl Acad Sci U S A. 2002;99(20):12600–5.CrossRefPubMedPubMedCentral
6.
Datta N, Pham QP, Sharma U, Sikavitsas VI, Jansen JA, Mikos AG. In vitro generated extracellular matrix and fluid shear stress synergistically enhance 3D osteoblastic differentiation. Proc Natl Acad Sci U S A. 2006;103(8):2488–93.CrossRefPubMedPubMedCentral
7.
Yu X, Botchwey EA, Levine EM, Pollack SR, Laurencin CT. Bioreactor-based bone tissue engineering: the influence of dynamic flow on osteoblast phenotypic expression and matrix mineralization. Proc Natl Acad Sci U S A. 2004;101(31):11203–8.CrossRefPubMedPubMedCentral
8.
Pavalko FM, Chen NX, Turner CH, Burr DB, Atkinson S, Hsieh YF, et al. Fluid shear-induced mechanical signaling in MC3T3-E1 osteoblasts requires cytoskeleton-integrin interactions. Am J Physiol. 1998;275(6 Pt 1):C1591–601.PubMed
9.
Astrof NS, Salas A, Shimaoka M, Chen J, Springer TA. Importance of force linkage in mechanochemistry of adhesion receptors. Biochemistry. 2006;45(50):15020–8.CrossRefPubMedPubMedCentral
10.
Bakker DP, van der Plaats A, Verkerke GJ, Busscher HJ, van der Mei HC. Comparison of velocity profiles for different flow chamber designs used in studies of microbial adhesion to surfaces. Appl Environ Microbiol. 2003;69(10):6280–7.CrossRefPubMedPubMedCentral
11.
Bannister SR, Lohmann CH, Liu Y, Sylvia VL, Cochran DL, Dean DD, et al. Shear force modulates osteoblast response to surface roughness. J Biomed Mater Res. 2002;60(1):167–74.CrossRefPubMed
12.
Becker J, Kirsch A, Schwarz F, Chatzinikolaidou M, Rothamel D, Lekovic V, et al. Bone apposition to titanium implants biocoated with recombinant human bone morphogenetic protein-2 (rhBMP-2). A pilot study in dogs. Clin Oral Investig. 2006;10(3):217–24.CrossRefPubMedPubMedCentral
13.
Hung CT, Allen FD, Pollack SR, Brighton CT. What is the role of the convective current density in the real-time calcium response of cultured bone cells to fluid flow? J Biomech. 1996;29(11):1403–9.CrossRefPubMed
14.
Ryder KD, Duncan RL. Parathyroid hormone enhances fluid shear-induced [Ca2+](i) signaling in osteoblastic cells through activation of mechanosensitive and voltage-sensitive Ca2+ channels. J Bone Miner Res. 2001;16(2):240–8.CrossRefPubMed
15.
Rath AL, Bonewald LF, Ling J, Jiang JX, Van Dyke ME, Nicolella DP. Correlation of cell strain in single osteocytes with intracellular calcium, but not intracellular nitric oxide, in response to fluid flow. J Biomech. 2010;43(8):1560–4.CrossRefPubMedPubMedCentral
16.
Klein-Nulend J, van der Plas A, Semeins CM, Ajubi NE, Frangos JA, Nijweide PJ, et al. Sensitivity of osteocytes to biomechanical stress in vitro. FASEB J. 1995;9(5):441–5.PubMed
17.
Klein-Nulend J, Semeins CM, Ajubi NE, Nijweide PJ, Burger EH. Pulsating fluid flow increases nitric oxide (NO) synthesis by osteocytes but not periosteal fibroblasts—correlation with prostaglandin upregulation. Biochem Biophys Res Commun. 1995;217(2):640–8.CrossRefPubMed
18.
Mcgarry JG, Klein-Nulend J, Mullender MG, Prendergast PJ. A comparison of strain and fluid shear stress in stimulating bone cell responses—a computational and experimental study. FASEB J. 2005;19(3):482–4.PubMed
19.
Nauman EA, Satcher RL, Keaveny TM, Halloran BP, Bikle DD. Osteoblasts respond to pulsatile fluid flow with short-term increases in PGE(2) but no change in mineralization. J Appl Physiol. 2001;90(5):1849–54.PubMed
20.
21.
Orr AW, Ginsberg MH, Shattil SJ, Deckmyn H, Schwartz MA. Matrix-specific suppression of integrin activation in shear stress signaling. Mol Biol Cell. 2006;17(11):4686–97.CrossRefPubMedPubMedCentral
22.
Chen NX, Ryder KD, Pavalko FM, Turner CH, Burr DB, Qiu J, et al. Ca(2+) regulates fluid shear-induced cytoskeletal reorganization and gene expression in osteoblasts. Am J Physiol Cell Physiol. 2000;278(5):C989–97.PubMed
23.
Hughes-Fulford M. Signal transduction and mechanical stress. Sci STKE. 2004;2004(249):RE12.PubMed
24.
Ruel J, Lemay J, Dumas G, Doillon C, Charara J. Development of a parallel plate flow chamber for studying cell behavior under pulsatile flow. ASAIO J. 1995;41(4):876–83.CrossRefPubMed
25.
Kazakidi A, Sherwin SJ, Weinberg PD. Effect of Reynolds number and flow division on patterns of haemodynamic wall shear stress near branch points in the descending thoracic aorta. J R Soc Interface. 2009;6(35):539–48.CrossRefPubMed
26.
27.
Usami S, Chen HH, Zhao Y, Chien S, Skalak R. Design and construction of a linear shear stress flow chamber. Ann Biomed Eng. 1993;21(1):77–83.CrossRefPubMed
28.
Sikavitsas VI, Bancroft GN, Holtorf HL, Jansen JA, Mikos AG. Mineralized matrix deposition by marrow stromal osteoblasts in 3D perfusion culture increases with increasing fluid shear forces. Proc Natl Acad Sci U S A. 2003;100(25):14683–8.CrossRefPubMedPubMedCentral
29.
Mcgarry JG, Klein-Nulend J, Prendergast PJ. The effect of cytoskeletal disruption on pulsatile fluid flow-induced nitric oxide and prostaglandin E2 release in osteocytes and osteoblasts. Biochem Biophys Res Commun. 2005;330(1):341–8.CrossRefPubMed
30.
Carvalho RS, Scott JE, Yen EH. The effects of mechanical stimulation on the distribution of beta 1 integrin and expression of beta 1-integrin mRNA in TE-85 human osteosarcoma cells. Arch Oral Biol. 1995;40(3):257–64.CrossRefPubMed
31.
Malone AM, Batra NN, Shivaram G, Kwon RY, You L, Kim CH, et al. The role of actin cytoskeleton in oscillatory fluid flow-induced signaling in MC3T3-E1 osteoblasts. Am J Physiol Cell Physiol. 2007;292(5):C1830–6.CrossRefPubMedPubMedCentral
32.
Lichtenstein N, Geiger B, Kam Z. Quantitative analysis of cytoskeletal organization by digital fluorescent microscopy. Cytometry A. 2003;54(1):8–18.CrossRefPubMed
33.
Jacobs CR, Yellowley CE, Davis BR, Zhou Z, Cimbala JM, Donahue HJ. Differential effect of steady versus oscillating flow on bone cells. J Biomech. 1998;31(11):969–76.CrossRefPubMedPubMedCentral
34.
Srinivasan S, Agans SC, King KA, Moy NY, Poliachik SL, Gross TS. Enabling bone formation in the aged skeleton via rest-inserted mechanical loading. Bone. 2003;33(6):946–55.CrossRefPubMed
35.
Papadaki M, Eskin SG. Effects of fluid shear stress on gene regulation of vascular cells. Biotechnol Prog. 1997;13(3):209–21.CrossRefPubMed
36.
James NL, Harrison DG, Nerem RM. Effects of shear on endothelial cell calcium in the presence and absence of ATP. FASEB J. 1995;9(10):968–73.PubMed
37.
Kämmerer PW, Lehnert M, Al-Nawas B, Kumar VV, Hagmann S, Alshihri A, et al. Osseoconductivity of a specific streptavidin-biotin-fibronectin surface coating of biotinylated titanium implants—a rabbit animal study. Clin Implant Dent Relat Res. 2015;17 Suppl 2:e601–12.CrossRefPubMed
Metadata
Title
Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up
Authors
P. W. Kämmerer
D. G. E. Thiem
A. Alshihri
G. H. Wittstock
R. Bader
B. Al-Nawas
M. O. Klein
Publication date
01-12-2017
Publisher
Springer Berlin Heidelberg
Published in
International Journal of Implant Dentistry / Issue 1/2017
Electronic ISSN: 2198-4034
DOI
https://doi.org/10.1186/s40729-017-0085-3

Other articles of this Issue 1/2017

International Journal of Implant Dentistry 1/2017 Go to the issue

Erratum

Erratum to: Osseointegration of standard and mini dental implants: a histomorphometric comparison

Research

The effect of membrane exposure on lateral ridge augmentation: a case-controlled study

Research

Electro-chemical deposition of nano hydroxyapatite-zinc coating on titanium metal substrate

Case report

Zirconia implants and peek restorations for the replacement of upper molars

Research

Dental implants and grafting success remain high despite large variations in maxillary sinus mucosal thickening

Research

Retrospective cohort study of rough-surface titanium implants with at least 25 years’ function