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Open Access 01-12-2018 | Research

Methylome of human senescent hematopoietic progenitors

Authors: Stephen Capone, Anthony R. Colombo, Benjamin K. Johnson, Tim J. Triche Jr., Giridharan Ramsingh

Published in: Experimental Hematology & Oncology | Issue 1/2018

Abstract

Senescence, a state of permanent cell cycle arrest, can be induced by DNA damage. This process, which was initially described in fibroblasts, is now recognized to occur in stem cells. It has been well characterized in cell lines, but there is currently very limited data available on human senescence in vivo. We recently reported that the expression of transposable elements (TE), including endogenous retroviruses, was up-regulated along with inflammatory genes in human senescent hematopoietic stem and progenitor cells (HSPCs) in vivo. The mechanism of regulation of TE expression is not completely understood, but changes in DNA methylation and chromatin modifications are known to alter their expression. In order to elucidate the molecular mechanisms for TE up-regulation after senescence of HSPCs, we employed whole-genome bisulfite sequencing in paired senescent and active human HSPCs in vivo from healthy subjects. We found that the senescent HSPCs exhibited hypomethylated regions in the genome, which were enriched for TEs. This is the first report characterizing the methylome of senescent human HSPCs.

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Title: Methylome of human senescent hematopoietic progenitors
Authors: Stephen Capone
Anthony R. Colombo
Benjamin K. Johnson
Tim J. Triche Jr.
Giridharan Ramsingh
Publication date: 01-12-2018
Publisher: BioMed Central
Published in: Experimental Hematology & Oncology / Issue 1/2018
Electronic ISSN: 2162-3619
DOI: https://doi.org/10.1186/s40164-018-0123-8

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