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European Journal of Medical Research

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Open Access 01-12-2020 | Vulvovaginal Candidiasis | Research

In vitro antifungal resistance profile of Candida strains isolated from Saudi women suffering from vulvovaginitis

Authors: Mohamed T. Yassin, Ashraf A. Mostafa, Abdulaziz A. Al-Askar, Rashad Bdeer

Published in: European Journal of Medical Research | Issue 1/2020

Abstract

Background

Vulvovaginal candidiasis (VVC) represents a universal health hazard that contributes to significant morbidity in women. Resistance of Candida to antifungal therapy has been reported as a public health problem. So, the objective of our current study is to detect resistance profile of different candidal strains.

Methods

In this study, isolated Candida strains were identified by conventional methods, confirmed by internal transcribed spacer (ITS) sequencing, and phylogenetically analyzed with reference strains in GenBank. Also, sensitivity of different Candida strains to common antifungal agents was evaluated by disc diffusion method.

Results

Candida albicans was identified as the most frequent strain (63%) followed by non-albicans strains, such as C. glabrata (20%), C. tropicalis (13%), and C. krusei (4%). Sensitivity of Candida strains (C. albicans, C. tropicalis and C. glabrata) to commonly used antifungal agents was evaluated through the disc diffusion method. C. glabrata was the most resistant strain and considered to be a multidrug-resistant pathogen, while both, C. albicans and C. tropicalis showed high susceptibility to terbinafine. In contrast, C. albicans showed resistance to fluconazole, clotrimazole, and nystatin, while C. tropicalis, considered as the most sensitive strain, was susceptible to all the antifungal agents tested except nystatin. Terbinafine was the most effective antifungal agent against both C. tropicalis and C. albicans, and hence its minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) for C. albicans and C. tropicalis were evaluated. MICs of terbinafine against C. albicans and C. tropicalis were 5 μg/ml and 2.5 μg/ml, while their MFCs were 10 μg/ml and 5 μg/ml, respectively.

Conclusion

The emergence of resistant Candida strains necessitates conduction of the antifungal susceptibility test prior to deciding the medication regime.

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Title: In vitro antifungal resistance profile of Candida strains isolated from Saudi women suffering from vulvovaginitis
Authors: Mohamed T. Yassin
Ashraf A. Mostafa
Abdulaziz A. Al-Askar
Rashad Bdeer
Publication date: 01-12-2020
Publisher: BioMed Central
Keywords: Vulvovaginal Candidiasis
Candida Balanitis
Candida Albicans
Fluconazole
Nystatin
Clotrimazole
Terbinafine
Published in: European Journal of Medical Research / Issue 1/2020
Electronic ISSN: 2047-783X
DOI: https://doi.org/10.1186/s40001-019-0399-0

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In vitro antifungal resistance profile of Candida strains isolated from Saudi women suffering from vulvovaginitis

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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