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European Journal of Medical Research
Published in: European Journal of Medical Research 1/2017

Open Access 01-12-2017 | Research

Platelet-rich plasma stimulates angiogenesis in mice which may promote hair growth

Authors: Hanxiao Cheng, Jufang Zhang, Jinsheng Li, Ming Jia, Yuyan Wang, Haiyan Shen

Published in: European Journal of Medical Research | Issue 1/2017

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Abstract

Background

Platelet-rich plasma (PRP) is an autologous concentration of human platelets in plasma. In this paper, we aimed to investigate the effect of PRP on hair growth.

Methods

Platelet-rich plasma and platelet-poor plasma were prepared by sterile centrifugation and injected into shaved dorsal skin of mice (n = 10). Saline injection was used in the control group. The length of randomly plucked hairs was measured at 8, 13, 18 days after PRP injection. Histological examination was preformed to observe the histologic changes of skins. The immunohistochemistry analysis of CD31 was performed to detect the changes of hair length and formation of new vessels.

Results

At 13 and 18 days after the last injection, the hair length of mice in PRP group (4.24 ± 0.60 and 8.29 ± 0.48 mm, respectively) was significantly longer compared with the control group (3.70 ± 0.52 and 7.21 ± 0.64 mm, p < 0.05). No significant difference in the hair length was found between the PPP group and the control (p > 0.05). In addition, the number of CD31-positive vessel in the PRP group (9.90 ± 0.60) was more than that in the control group (8.60 ± 2.34, p < 0.05).

Conclusion

Platelet-rich plasma might promote hair length growth and increase the number of hair follicles by inducing angiogenesis.
Literature
1.
Dituri F, Mazzocca A, Lupo L, Edling CE, Azzariti A, Antonaci S, Falasca M, Giannelli G. PI3K class IB controls the cell cycle checkpoint promoting cell proliferation in hepatocellular carcinoma. Int J Cancer. 2012;130(11):2505–13.CrossRefPubMed
2.
Wang W, Zhao L-J, Tan Y-X, Ren H, Qi Z-T. MiR-138 induces cell cycle arrest by targeting cyclin D3 in hepatocellular carcinoma. Carcinogenesis. 2012;33(5):1113–20.CrossRefPubMedPubMedCentral
3.
Mammoto T, Jiang A, Jiang E, Mammoto A. Platelet rich plasma extract promotes angiogenesis through the angiopoietin1-Tie2 pathway. Microvasc Res. 2013;89(9):15–24.CrossRefPubMed
4.
5.
Uebel CO, da Silva JB, Cantarelli D, Martins P. The role of platelet plasma growth factors in male pattern baldness surgery. Plast Reconstr Surg. 2006;118(6):1458–66.CrossRefPubMed
6.
Li ZJ, Choi HI, Choi DK, Sohn KC, Im M, Seo YJ, Lee YH, Lee JH, Lee Y. Autologous platelet-rich plasma: a potential therapeutic tool for promoting hair growth. Dermatol Surg. 2012;38(7pt1):1040–6.CrossRefPubMed
7.
Miao Y, Sun YB, Sun XJ, Du BJ, Jiang JD, Hu ZQ. Promotional effect of platelet-rich plasma on hair follicle reconstitution in vivo. Dermatol Surg. 2013;39(12):1868–76.CrossRefPubMed
8.
Sommeling C, Heyneman A, Hoeksema H, Verbelen J, Stillaert F, Monstrey S. The use of platelet-rich plasma in plastic surgery: a systematic review. J Plast Reconstr Aesthet Surg. 2013;66(3):301–11.CrossRefPubMed
9.
Cervelli V, Gentile P, Scioli MG, Grimaldi M, Casciani CU, Spagnoli LG, Orlandi A. Application of platelet-rich plasma in plastic surgery: clinical and in vitro evaluation. Tissue Eng Part C Methods. 2009;15(4):625–34.CrossRefPubMed
10.
Marx RE. Platelet-rich plasma: evidence to support its use. J Oral Maxillofac Surg. 2004;62(4):489–96.CrossRefPubMed
11.
Kushida S, Kakudo N, Morimoto N, Hara T, Ogawa T, Mitsui T, Kusumoto K. Platelet and growth factor concentrations in activated platelet-rich plasma: a comparison of seven commercial separation systems. J Artif Organs. 2014;17:186–92.CrossRefPubMed
12.
Kaux JF, Libertiaux V, Croisier JL, Crielaard JM. Platelet-rich plasma (PRP) to treat chronic patellar tendinopathies: comparison of a single versus two closely-timed injections. Muscles Ligaments Tendons J. 2015;5(4):297–8.PubMed
13.
Mooren RE, Hendriks EJ, van den Beucken JJ, Merkx MA, Meijer GJ, Jansen JA, Stoelinga PJ. The effect of platelet-rich plasma in vitro on primary cells: rat osteoblast-like cells and human endothelial cells. Tissue Eng Part A. 2010;16(10):3159–72.CrossRefPubMed
14.
DeLong JM, Russell RP, Mazzocca AD. Platelet-rich plasma: the PAW classification system. Arthroscopy. 2012;28(7):998–1009.CrossRefPubMed
15.
Ranly DM, McMillan J, Keller T, Lohmann CH, Meunch T, Cochran DL, Schwartz Z, Boyan BD. Platelet-derived growth factor inhibits demineralized bone matrix-induced intramuscular cartilage and bone formationA study of immunocompromised mice. J Bone Joint Surg. 2005;87(9):2052–64.CrossRefPubMed
16.
Han B, Woodell-May J, Ponticiello M, Yang Z, Nimni M. The effect of thrombin activation of platelet-rich plasma on demineralized bone matrix osteoinductivity. J Bone Joint Surg. 2009;91(6):1459–70.CrossRefPubMed
17.
Marini MG, Perrini C, Esposti P, Corradetti B, Bizzaro D, Riccaboni P, Fantinato E, Urbani G, Gelati G, Cremonesi F. Effects of platelet-rich plasma in a model of bovine endometrial inflammation in vitro. Reprod Biol Endocrinol. 2016;14(1):58.CrossRefPubMedPubMedCentral
18.
Marx RE. Platelet-rich plasma (PRP): what is PRP and what is not PRP? Implant Dent. 2001;10(4):225–8.CrossRefPubMed
19.
Frelinger Iii AL, Torres AS, Caiafa A, Morton CA, Bernylang MA, Gerrits AJ, Carmichael SL, Neculaes VB, Michelson AD. Platelet-rich plasma stimulated by pulse electric fields: platelet activation, procoagulant markers, growth factor release and cell proliferation. Platelets. 2015;27(2):1–8.CrossRef
20.
Foster TE, Puskas BL, Mandelbaum BR, Gerhardt MB, Rodeo SA. Platelet-rich plasma from basic science to clinical applications. Am J Sports Med. 2009;37(11):2259–72.CrossRefPubMed
21.
Artitua E, Andia I, Ardanza B, Nurden P, Nurden A. Autologous platelets as a source of proteins for healing and tissue regeneration. Thromb Haemost. 2004;91(4):4–15.
22.
Eppley BL, Woodell JE, Higgins J. Platelet quantification and growth factor analysis from platelet-rich plasma: implications for wound healing. Plast Reconstr Surg. 2004;114(6):1502–8.CrossRefPubMed
23.
Nikolidakis D, Jansen JA. The biology of platelet-rich plasma and its application in oral surgery: literature review. Tissue Eng Part B Rev. 2008;14(3):249–58.CrossRefPubMed
24.
Kim DH, Je YJ, Kim CD, Lee YH, Seo YJ, Lee JH, Lee Y. Can platelet-rich plasma be used for skin rejuvenation? Evaluation of effects of platelet-rich plasma on human dermal fibroblast. Ann Dermatol. 2011;23(4):424–31.CrossRefPubMedPubMedCentral
25.
Takakura N, Yoshida H, Kunisada T, Nishikawa S, Nishikawa S-I. Involvement of platelet-derived growth factor receptor-α in hair canal formation. J Investig Dermatol. 1996;107(5):770–7.CrossRefPubMed
26.
27.
Cocchi MA, Bezzola P, Sorbellini E, Trink A, Labanca M, Bonazza V, Rinaldi F. Platelet rich plasma stimulates human hair growth in vitro. Ital J Anat Embryol. 2013;118(2):57.
28.
29.
Langer H, May AE, Daub K, Heinzmann U, Lang P, Schumm M, Vestweber D, Massberg S, Schönberger T, Pfisterer I. Adherent platelets recruit and induce differentiation of murine embryonic endothelial progenitor cells to mature endothelial cells in vitro. Circ Res. 2006;98(2):e2–10.CrossRefPubMed
30.
Paus R, Foitzik K. In search of the “hair cycle clock”: a guided tour. Differentiation. 2005;72(9–10):489–511.
Metadata
Title
Platelet-rich plasma stimulates angiogenesis in mice which may promote hair growth
Authors
Hanxiao Cheng
Jufang Zhang
Jinsheng Li
Ming Jia
Yuyan Wang
Haiyan Shen
Publication date
01-12-2017
Publisher
BioMed Central
Published in
European Journal of Medical Research / Issue 1/2017
Electronic ISSN: 2047-783X
DOI
https://doi.org/10.1186/s40001-017-0278-5

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