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Reproductive Biology and Endocrinology
Published in: Reproductive Biology and Endocrinology 1/2016

Open Access 01-12-2016 | Research

Effects of platelet-rich plasma in a model of bovine endometrial inflammation in vitro

Authors: Maria Giovanna Marini, Claudia Perrini, Paola Esposti, Bruna Corradetti, Davide Bizzaro, Pietro Riccaboni, Eleonora Fantinato, Giuseppe Urbani, Giorgio Gelati, Fausto Cremonesi, Anna Lange-Consiglio

Published in: Reproductive Biology and Endocrinology | Issue 1/2016

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Abstract

Background

Endometritis reduces fertility and is responsible for major economic losses in beef and dairy industries. The aim of this study was to evaluate an alternative therapy using platelet-rich plasma (PRP). PRP was tested in vivo, after bovine intrauterine administration, and in vitro on endometrial cells.

Methods

Bovine endometrial cells were cultured until passage (P) 10 with 5 % or 10 % PRP. Effect of PRP on endometrial cell proliferation and on the expression of genes [prostaglandin-endoperoxide synthase 2 (COX2), tumor protein p53 (TP53), oestrogen receptors (ER-α and ER-β), progesterone receptor (PR) and c-Myc] involved in the regulation of oestrus cycle and fetal-maternal interaction were evaluated. Moreover, to evaluate the ability of PRP to counteract inflammation, 10 and 100 ng/ml of bacterial endotoxin lipopolysaccharide (LPS) were used to inflame endometrial cells in vitro for 1, 6, 12, 24 and 48 h. The expression of genes such as interleukin 1β (IL-1β), interleukin-8 (IL-8), inducible nitric oxide synthase (iNOS), prostaglandin-endoperoxide synthase 2 (COX2/PTGS2), and the release of PGE-2, IL-1β and IL-8 were evaluated.

Results

In vivo treatment with PRP increased the detection of PR. In vitro, 5 % PRP at passage 5 increased proliferation rate and induced a significant increase in the expression of all studied genes. Furthermore, the results revealed that 10 ng/ml of LPS is the most effective dose to obtain an inflammatory response, and that PRP treatment significantly down regulated the expression of pro-inflammatory genes.

Conclusion

This study lays the foundations for the potential treatment of endometritis with PRP in vivo.
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Metadata
Title
Effects of platelet-rich plasma in a model of bovine endometrial inflammation in vitro
Authors
Maria Giovanna Marini
Claudia Perrini
Paola Esposti
Bruna Corradetti
Davide Bizzaro
Pietro Riccaboni
Eleonora Fantinato
Giuseppe Urbani
Giorgio Gelati
Fausto Cremonesi
Anna Lange-Consiglio
Publication date
01-12-2016
Publisher
BioMed Central
Published in
Reproductive Biology and Endocrinology / Issue 1/2016
Electronic ISSN: 1477-7827
DOI
https://doi.org/10.1186/s12958-016-0195-4

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