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Open Access 01-12-2015 | Research

The molecular fingerprint of lung inflammation after blunt chest trauma

Authors: Christian Ehrnthaller, Michael Flierl, Mario Perl, Stephanie Denk, Heike Unnewehr, Peter A. Ward, Peter Radermacher, Anita Ignatius, Florian Gebhard, Arul Chinnaiyan, Markus Huber-Lang

Published in: European Journal of Medical Research | Issue 1/2015

Abstract

Background

After severe blunt chest trauma, the development of an acute lung injury (ALI) is often associated with severe or even lethal complications. Especially in multiple injured patients after blunt chest trauma ALI/ARDS [acute respiratory distress syndrome (ARDS)] is frequent. However, in the initial posttraumatic phase, inflammatory clinical signs are often not apparent and underlying changes in gene-expression profile are unknown.

Methods

Therefore, inflammation in lung tissue following blunt chest trauma was characterized in a well-defined bilateral lung injury model. Using DNA microarrays representing 9240 genes, the temporal sequence of blunt chest trauma-induced gene-expression patterns in lung tissue was examined.

Results

The results suggest an activation of a highly complex transcriptional program in response to chest trauma. Chest trauma led to elevated expression levels of inflammatory and coagulatory proteins (such as TNFα receptor, IL-1α, IL-1β, C3, NF-κB and plasminogen activator). However, upregulation of proteins was found, usually incoherent of exerting effects in blunt thoracic trauma (pendrin, resistin, metallothionein and glucocorticoid-induced leucine zipper). Furthermore, significant downregulation was observed as early as 10 min after trauma for cytokines and complement factors (LCR-1, C4) as well as for intracellular signaling molecules (inhibitory protein phosphatase) and ion-channels (voltage-dependent Ca²⁺ channel).

Conclusions

Taken together, the provided global perspective of the inflammatory response following blunt chest trauma could provide a molecular framework for future research in trauma pathophysiology.

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Title: The molecular fingerprint of lung inflammation after blunt chest trauma
Authors: Christian Ehrnthaller
Michael Flierl
Mario Perl
Stephanie Denk
Heike Unnewehr
Peter A. Ward
Peter Radermacher
Anita Ignatius
Florian Gebhard
Arul Chinnaiyan
Markus Huber-Lang
Publication date: 01-12-2015
Publisher: BioMed Central
Published in: European Journal of Medical Research / Issue 1/2015
Electronic ISSN: 2047-783X
DOI: https://doi.org/10.1186/s40001-015-0164-y

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

The molecular fingerprint of lung inflammation after blunt chest trauma

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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