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EJNMMI Research

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Open Access 01-12-2015 | Original research

Non-invasive imaging of implanted peritoneal carcinomatosis in mice using PET and bioluminescence imaging

Authors: J. Stollfuss, N. Landvogt, M. Abenstein, S. Ziegler, M. Schwaiger, R. Senekowitsch-Schmidtke, H. Wieder

Published in: EJNMMI Research | Issue 1/2015

Abstract

Background

Non-invasive imaging of peritoneal carcinomatosis remains challenging. The aim of this study was to compare positron emission tomography (PET) and bioluminescence imaging (BLI) for the early detection of peritoneal carcinomatosis in a mouse model.

Methods

Female nude mice were inoculated intraperitoneally with 1×10⁷ HSC45-M2-luc gastric cancer cells. The cells were stably transfected with the gene coding for firefly luciferase. Tumour development was monitored using PET and BLI and in two subgroups, on days 3 and 4 or on days 6 and 7 after tumour cell inoculation. Tumour nodules found on post mortem examination served as the reference standard for evaluating the images.

Results

PET detected 58/82 lesions (sensitivity 71 %). This method detected all (100 %) nodules larger than 6 mm, 88 % of nodules in the range of >2–4 mm, and even 58 % of small nodules measuring only 1–2 mm. BLI identified a total of 40/82 lesions (sensitivity 49 %). The difference between PET and BLI was statistically significant at p < 0.05 (PET/BLI chi-square 8.2).

Conclusions

PET was more sensitive than BLI for the detection of early peritoneal carcinomatosis in our mouse model. The sensitivity of BLI largely depended on the site of the lesions in relation to the imaging device.

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Title: Non-invasive imaging of implanted peritoneal carcinomatosis in mice using PET and bioluminescence imaging
Authors: J. Stollfuss
N. Landvogt
M. Abenstein
S. Ziegler
M. Schwaiger
R. Senekowitsch-Schmidtke
H. Wieder
Publication date: 01-12-2015
Publisher: Springer Berlin Heidelberg
Published in: EJNMMI Research / Issue 1/2015
Electronic ISSN: 2191-219X
DOI: https://doi.org/10.1186/s13550-015-0125-z

At a glance: The STEP trials

Springer Medicine

Non-invasive imaging of implanted peritoneal carcinomatosis in mice using PET and bioluminescence imaging

Abstract

Background

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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