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EJNMMI Research
Published in: EJNMMI Research 1/2015

Open Access 01-12-2015 | Original research

64Cu-DOTA-trastuzumab PET imaging and HER2 specificity of brain metastases in HER2-positive breast cancer patients

Authors: Hiroaki Kurihara, Akinobu Hamada, Masayuki Yoshida, Schuichi Shimma, Jun Hashimoto, Kan Yonemori, Hitomi Tani, Yasuji Miyakita, Yousuke Kanayama, Yasuhiro Wada, Makoto Kodaira, Mayu Yunokawa, Harukaze Yamamoto, Chikako Shimizu, Kazuhiro Takahashi, Yasuyoshi Watanabe, Yasuhiro Fujiwara, Kenji Tamura

Published in: EJNMMI Research | Issue 1/2015

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Abstract

Background

The purpose of this study was to determine whether brain metastases from HER2-positive breast cancer could be detected noninvasively using positron emission tomography (PET) with 64Cu-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA)-trastuzumab.

Methods

PET was performed on five patients with brain metastases from HER2-positive breast cancer, at 24 or 48 h after the injection of approximately 130 MBq of the probe 64Cu-DOTA-trastuzumab. Radioactivity in metastatic brain tumors was evaluated based on PET images in five patients. Autoradiography, immunohistochemistry (IHC), and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis were performed in one surgical case to confirm HER2 specificity of 64Cu-DOTA-trastuzumab.

Results

Metastatic brain lesions could be visualized by 64Cu-DOTA-trastuzumab PET in all of five cases, which might indicated that trastuzumab passes through the blood-brain barrier (BBB). The HER2 specificity of 64Cu-DOTA-trastuzumab was demonstrated in one patient by autoradiography, immunohistochemistry, and LC-MS/MS.

Conclusions

Cu-DOTA-trastuzumab PET could be a potential noninvasive procedure for serial identification of metastatic brain lesions in patients with HER2-positive breast cancer.

Trial registration

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Metadata
Title
64Cu-DOTA-trastuzumab PET imaging and HER2 specificity of brain metastases in HER2-positive breast cancer patients
Authors
Hiroaki Kurihara
Akinobu Hamada
Masayuki Yoshida
Schuichi Shimma
Jun Hashimoto
Kan Yonemori
Hitomi Tani
Yasuji Miyakita
Yousuke Kanayama
Yasuhiro Wada
Makoto Kodaira
Mayu Yunokawa
Harukaze Yamamoto
Chikako Shimizu
Kazuhiro Takahashi
Yasuyoshi Watanabe
Yasuhiro Fujiwara
Kenji Tamura
Publication date
01-12-2015
Publisher
Springer Berlin Heidelberg
Published in
EJNMMI Research / Issue 1/2015
Electronic ISSN: 2191-219X
DOI
https://doi.org/10.1186/s13550-015-0082-6

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