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01-12-2021 | Helicobacter Pylori | Research

Helicobacter pylori in the Indonesian Malay’s descendants might be imported from other ethnicities

Authors: Ari Fahrial Syam, Langgeng Agung Waskito, Yudith Annisa Ayu Rezkitha, Rentha Monica Simamora, Fauzi Yusuf, Kanserina Esthera Danchi, Ahmad Fuad Bakry, Arnelis, Erwin Mulya, Gontar Alamsyah Siregar, Titong Sugihartono, Hasan Maulahela, Dalla Doohan, Muhammad Miftahussurur, Yoshio Yamaoka

Published in: Gut Pathogens | Issue 1/2021

Abstract

Background

Even though the incidence of H. pylori infection among Malays in the Malay Peninsula is low, we observed a high H. pylori prevalence in Sumatra, which is the main residence of Indonesian Malays. H. pylori prevalence among Indonesian Malay descendants was investigated.

Results

Using a combination of five tests, 232 recruited participants were tested for H- pylori and participants were considered positive if at least one test positive. The results showed that the overall H. pylori prevalence was 17.2%. Participants were then categorized into Malay (Aceh, Malay, and Minang), Java (Javanese and Sundanese), Nias, and Bataknese groups. The prevalence of H. pylori was very low among the Malay group (2.8%) and no H. pylori was observed among the Aceh. Similarly, no H. pylori was observed among the Java group. However, the prevalence of H. pylori was high among the Bataknese (52.2%) and moderate among the Nias (6.1%). Multilocus sequence typing showed that H. pylori in Indonesian Malays classified as hpEastAsia with a subpopulation of hspMaori, suggesting that the isolated H. pylori were not a specific Malays H. pylori.

Conclusions

Even though the ethnic groups live together as a community, we observed an extremely low H. pylori infection rate among Indonesian Malay descendants with no specific Indonesian Malay H. pylori. The results suggest that H. pylori was not originally among these groups and H. pylori was imported from other ethnic groups.

Amieva M, Peek RM. Pathobiology of Helicobacter pylori-Induced Gastric Cancer. Gastroenterology. W.B. Saunders; 2016. pp. 64–78. https://doi.org/10.1053/j.gastro.2015.09.004

Hooi JKY, Lai WY, Ng WK, Suen MMY, Underwood FE, Tanyingoh D, et al. Global prevalence of helicobacter pylori infection: systematic review and meta-analysis. Gastroenterology. 2017;153:420–9. https://doi.org/10.1053/j.gastro.2017.04.022.CrossRefPubMed

Graham DY. History of Helicobacter pylori, duodenal ulcer, gastric ulcer and gastric cancer. World J Gastroenterol. 2014;20:5191–204. https://doi.org/10.3748/wjg.v20.i18.5191.CrossRefPubMedPubMedCentral

Hatin WI, Nur-Shafawati AR, Zahri MK, Xu S, Jin L, Tan SG, et al. Population genetic structure of peninsular Malaysia Malay sub-ethnic groups. PLoS ONE. 2011;6:e18312. https://doi.org/10.1371/journal.pone.0018312.CrossRefPubMedPubMedCentral

Omar AH. Languange and Literature. Encycl Malaysia. 2004.

Din MAO. The Malay Origin: Rewrite Its History. J Melayu. 2011;7:1–81.

Fix AG. Malayan paleosociology: implications for patterns of genetic variation amongst the Orang Asli. Am Anthropol. 1995;97:313–23.CrossRef

Sysling F. Racial Science and Human Diversity in Colonial Indonesia. NUS Press; 2016.

Hatin WI, Nur-Shafawati AR, Etemad A, Jin W, Qin P, Xu S, et al. A genome wide pattern of population structure and admixture in peninsular Malaysia Malays. Hugo J. 2014;8:5. https://doi.org/10.1186/s11568-014-0005-z.CrossRefPubMedPubMedCentral

10.

Pitoyo AJ, Triwahyudi H. Dinamika Perkembangan Etnis di Indonesia Dalam Konteks Persatuan Negara. Populasi. 2017;25:64–81.CrossRef

11.

Goh KL, Parasakthi N. The racial cohort phenomenon: seroepidemiology of Helicobacter pylori infection in a multiracial South-East Asian country. Eur J Gastroenterol Hepatol. 2001;13:177–83. https://doi.org/10.1097/00042737-200102000-00014.CrossRefPubMed

12.

Syam AF, Miftahussurur M, Makmun D, Nusi IA, Zain LH, et al. Risk Factors and Prevalence of Helicobacter pylori in Five Largest Islands of Indonesia: A Preliminary Study. PLoS ONE. 2015;10:e0140186. https://doi.org/10.1371/journal.pone.0140186.CrossRefPubMedPubMedCentral

13.

Miftahussurur M, Tuda J, Suzuki R, Kido Y, Kawamoto F, Matsuda M, et al. Extremely low Helicobacter pylori prevalence in North Sulawesi, Indonesia and identification of a Maori-tribe type strain: a cross sectional study. Gut Pathog. 2014;6:42. https://doi.org/10.1186/s13099-014-0042-0.CrossRefPubMedPubMedCentral

14.

Moodley Y, Linz B, Yamaoka Y, Windsor HM, Breurec S, Wu JY, et al. The peopling of the Pacific from a bacterial perspective. Science. 2009;323:527–30. https://doi.org/10.1126/science.1166083.CrossRefPubMedPubMedCentral

15.

Sasidharan S, Jo S, Lachumy T, Ravichandran M, Latha Y, Rao S, et al. Epidemiology of Helicobacter pylori among multiracial community in Northern Peninsular, Malaysia : effect of age across race and gender. Asian Pac J Trop Med. 2011;4:72–5. https://doi.org/10.1016/S1995-7645(11)60037-0.CrossRefPubMed

16.

Thevakumar K, Chandren JR, Perez-Perez GI, Chua EG, Teh LK, Salleh MZ, et al. Assessment of Risk and Sero-Prevalence of Helicobacter pylori Colonization among Remote Orang Asli Tribes in Peninsula Malaysia. PLoS ONE. 2016;11:e0159830. https://doi.org/10.1371/journal.pone.0159830.CrossRefPubMedPubMedCentral

17.

Rajendra S, Ackroyd R, Robertson IK, Ho JJ, Karim N, Kutty KM. Helicobacter pylori, ethnicity, and the gastroesophageal reflux disease spectrum: A study from the East. Helicobacter. 2007;12:177–83. https://doi.org/10.1111/j.1523-5378.2007.00489.x.CrossRefPubMed

18.

Kang JY, Yeoh KG, Ho KY, Guan R, Lim TP, Quak SH, et al. Racial differences in Helicobacter pylori seroprevalence in Singapore: correlation with differences in peptic ulcer frequency. J Gastroenterol Hepatol. 1997;12:655–9. https://doi.org/10.1111/j.1440-1746.1997.tb00530.x.CrossRefPubMed

19.

Maran S, Lee YY, Xu SH, Raj MS, Abdul Majid N, Choo KE, et al. Towards understanding the low prevalence of Helicobacter pylori in Malays: Genetic variants among Helicobacter pylori -negative ethnic Malays in the north-eastern region of Peninsular Malaysia and Han Chinese and South Indians. J Dig Dis. 2013;14:196–202. https://doi.org/10.1111/1751-2980.12023.CrossRefPubMed

20.

Schmidt HMA, Ha DM, Taylor EF, Kovach Z, Goh KL, Fock KM, et al. Variation in human genetic polymorphisms, their association with Helicobacter pylori acquisition and gastric cancer in a multi-ethnic country. J Gastroenterol Hepatol. 2011;26:1725–32. https://doi.org/10.1111/j.1440-1746.2011.06799.x.CrossRefPubMed

21.

Goh KL. Lessons learnt from the epidemiology of Helicobacter pylori infection in Malaysia: JGHF Marshall and Warren Lecture 2017. J Gastroenterol Hepatol (Australia). 2018. https://doi.org/10.1111/jgh.14131.CrossRefPubMed

22.

Ansari S, Yamaoka Y. Helicobacter pylori virulence factors exploiting gastric colonization and its pathogenicity. Toxins. 2019. https://doi.org/10.3390/toxins11110677.CrossRefPubMedPubMedCentral

23.

Lee YY, Ismail AW, Mustaffa N, Musa KI, Majid NA, Choo KE, et al. Sociocultural and dietary practices among malay subjects in the north-eastern region of peninsular Malaysia: a region of low prevalence of Helicobacter pylori Infection. Helicobacter. 2012;17:54–61. https://doi.org/10.1111/j.1523-5378.2011.00917.x.CrossRefPubMed

24.

Sairam K, Rao CV, Goel RK. Effect of Centella asiatica Linn on physical and chemical factors induced gastric ulceration and secretion in rats. Indian J Exp Biol. 2001;39:137–42.PubMed

25.

Pasaribu KM, Gea S, Ilyas S, Tamrin T, Sarumaha AA, Sembiring A, et al. Fabrication and in-vivo study of micro-colloidal Zanthoxylum acanthopodium-loaded bacterial cellulose as a burn wound dressing. Polymers (Basel). 2020. https://doi.org/10.3390/polym12071436.CrossRefPubMedPubMedCentral

26.

Majumder M, Sharma HK, Zaman K, Lyngdoh W. Evaluation of physico-chemical properties and antibacterial activity of the essential oil obtained from the fruits of Zanthoxyllum acanthopodium DC. Collected from Meghalaya, India. TIC. 2014;1: e10.

27.

Yurliasni Y. Aktivitas Antimikroba Khamir Asal Dadih (susu kerbau fermentasi) Terhadap Beberapa Bakteri Patogen. J Agripet. 2010;10:19–24. https://doi.org/10.17969/agripet.v10i1.633.CrossRef

28.

Leng J. Prehistory of the Indo-Malaysian Archipelago. Peter Bellwood. 1997. Revised edition. University of Hawai’i Press, Honolulu, x + 385 pp., 92 figures, 61 plates, 3 tables, notes, references, index. $58.00 (cloth), $32.95 (paper). Am Antiq. 1998;63: 518–519. https://doi.org/10.2307/2694651

29.

Goto Y, Syam AF, Darnindro N, Hapsari FCP. Risk factors for and prevalence of helicobacter pylori infection among healthy inhabitants in Northern Jakarta Indonesia. Asian Pacific J Cancer Prev. 2016;17:4469–75.

30.

Yang X, Nishibayashi H, Takeshita T, Morimoto K. Prevalence of Helicobacter pylori infection in Japan: Relation to educational levels and hygienic conditions. Environ Health Prev Med. 1999;3:202–6. https://doi.org/10.1007/BF02932259.CrossRefPubMedPubMedCentral

31.

Karita M, Teramukai S, Matsumoto S. Risk of Helicobacter pylori transmission from drinking well water is higher than that from infected intrafamilial members in Japan. Dig Dis Sci. 2003;48:1062–7. https://doi.org/10.1023/A:1023752326137.CrossRefPubMed

32.

Miftahussurur M. Noninvasive Helicobacter pylori Diagnostic Methods in Indonesia. Gut and Liver. Editorial Office of Gut and Liver; 2020. pp. 553–559. https://doi.org/10.5009/GNL19264

33.

Makmun D. Present status of endoscopy, therapeutic endoscopy and the endoscopy training system in Indonesia. Dig Endosc. 2014;26:2–9. https://doi.org/10.1111/den.12245.CrossRefPubMed

34.

Juniawan RFD, Awalia A. Henoch-Schönlein purpura: management and complication. Biomol Heal Sci J. 2020;3:113–1115.CrossRef

35.

Miftahussurur M, Waskito LA, Syam AF, Nusi IA, Wibawa I, Rezkitha YAA, et al. Analysis of risks of gastric cancer by gastric mucosa among Indonesian ethnic groups. PLoS ONE. 2019;14:1–19. https://doi.org/10.1371/journal.pone.0216670.CrossRef

36.

Miftahussurur M, Syam AF, Nusi IA, Makmun D, Waskito LA, Zein LH, et al. Surveillance of Helicobacter pylori antibiotic susceptibility in Indonesia: different resistance types among regions and with novel genetic mutations. PLoS One. 2016. https://doi.org/10.1371/journal.pone.0166199.CrossRefPubMedPubMedCentral

37.

Dixon MF, Genta RM, Yardley JH, Correa P. Classification and grading of gastritis. The updated Sydney System. International Workshop on the Histopathology of Gastritis, Houston 1994. Am J Surg Pathol. 1996;20: 1161–1181.

38.

Uchida T, Kanada R, Tsukamoto Y, Hijiya N, Matsuura K, Yano S, et al. Immunohistochemical diagnosis of the cagA-gene genotype of Helicobacter pylori with anti-East Asian CagA-specific antibody. Cancer Sci. 2007;98:521–8. https://doi.org/10.1111/j.1349-7006.2007.00415.x.CrossRefPubMed

39.

Vilaichone RK, Mahachai V, Shiota S, Uchida T, Ratanachu-ek T, Tshering L, et al. Extremely high prevalence of Helicobacter pylori infection in Bhutan. World J Gastroenterol. 2013;19:2806–10. https://doi.org/10.3748/wjg.v19.i18.2806.CrossRefPubMedPubMedCentral

40.

Shiota S, Matsunari O, Watada M, Yamaoka Y. Serum Helicobacter pylori CagA antibody as a biomarker for gastric cancer in east-Asian countries. Futur Microbiol. 2010;5:1885–93. https://doi.org/10.2217/fmb.10.135.CrossRef

41.

Shiota S, Cruz M, Abreu JA, Mitsui T, Terao H, Disla M, et al. Virulence genes of Helicobacter pylori in the Dominican Republic. J Med Microbiol. 2014;63:1189–96. https://doi.org/10.1099/jmm.0.075275-0.CrossRefPubMedPubMedCentral

42.

Nguyen TL, Uchida T, Tsukamoto Y, Trinh DT, Ta L, Mai BH, et al. Helicobacter pylori infection and gastroduodenal diseases in Vietnam: a cross-sectional, hospital-based study. BMC Gastroenterol. 2010;10:114. https://doi.org/10.1186/1471-230X-10-114.CrossRefPubMedPubMedCentral

43.

Miftahussurur M, Syam AF, Makmun D, Nusi IA, Zein LH, et al. Helicobacter pylori virulence genes in the five largest islands of Indonesia. Gut Pathog. 2015;7:26. https://doi.org/10.1186/s13099-015-0072-2.CrossRefPubMedPubMedCentral

44.

Miftahussurur M, Nusi IA, Akil F, Syam AFAF, Wibawa ID, Rezkitha YA, et al. Gastric mucosal status in populations with a low prevalence of Helicobacter pylori in Indonesia. PLoS One. 2017;12:1–15. https://doi.org/10.1371/journal.pone.0176203.CrossRef

45.

Pritchard JK, Stephens M, Donnelly P. Inference of population structure using multilocus genotype data. Genetics. 2000;155:945–59.CrossRefPubMedPubMedCentral

46.

Linz B, Balloux F, Moodley Y, Manica A, Liu H, Roumagnac P, et al. An African origin for the intimate association between humans and Helicobacter pylori. Nature. 2007;445:915–8. https://doi.org/10.1038/nature05562.CrossRefPubMedPubMedCentral

47.

Falush D, Wirth T, Linz B, Pritchard JK, Stephens M, Kidd M, et al. Traces of human migrations in Helicobacter pylori populations. Science. 2003;299:1582–5. https://doi.org/10.1126/science.1080857.CrossRefPubMed

48.

Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol. 2016;33:1870–4. https://doi.org/10.1093/molbev/msw054.CrossRefPubMedPubMedCentral

49.

Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987;4:406–25. https://doi.org/10.1093/oxfordjournals.molbev.a040454.CrossRefPubMed

50.

Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol. 1980;16:111–20. https://doi.org/10.1007/BF01731581.CrossRefPubMed

Title: Helicobacter pylori in the Indonesian Malay’s descendants might be imported from other ethnicities
Authors: Ari Fahrial Syam
Langgeng Agung Waskito
Yudith Annisa Ayu Rezkitha
Rentha Monica Simamora
Fauzi Yusuf
Kanserina Esthera Danchi
Ahmad Fuad Bakry
Arnelis
Erwin Mulya
Gontar Alamsyah Siregar
Titong Sugihartono
Hasan Maulahela
Dalla Doohan
Muhammad Miftahussurur
Yoshio Yamaoka
Publication date: 01-12-2021
Publisher: BioMed Central
Keyword: Helicobacter Pylori
Published in: Gut Pathogens / Issue 1/2021
Electronic ISSN: 1757-4749
DOI: https://doi.org/10.1186/s13099-021-00432-6

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