Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
Gut Pathogens
Published in: Gut Pathogens 1/2018

Open Access 01-12-2018 | Research

Cecal microbiome profile altered by Salmonella enterica, serovar Enteritidis inoculation in chicken

Authors: Liying Liu, Lili Lin, Linna Zheng, Hui Tang, Xinzhong Fan, Nianguo Xue, Min Li, Min Liu, Xianyao Li

Published in: Gut Pathogens | Issue 1/2018

Login to get access

Abstract

Background

Salmonella enterica, serovar Enteritidis (S. Enteritidis), an important zoonotic foodborne pathogen, can affect the microbiota of the chicken intestine and cause many enteric diseases, such as acute gastroenteritis. The gut microbiota contributes to the development and function of the host immune system and competes with pathogenic microbes. The interaction between S. Enteritidis and the host cecal microbiota is still not fully understood. We investigated the microbiome composition in both treated and control groups through 16S ribosomal RNA (rRNA) gene sequencing at 1, 3, 7, 14, 21, 28, and 35 days post-S. Enteritidis inoculation (dpi) in the current study.

Results

Chao1 richness and Shannon diversity significantly increased with chicken development in both the treated and control groups (P < 0.05). The Chao1 index was significantly lower in the treated group than that in the control group at 14 dpi (P < 0.05). Phyla Proteobacteria and Firmicutes were most dominant at 1 and 3 dpi. S. Enteritidis inoculation influenced cecal microbiota mainly at 7 and 14 dpi. S. Enteritidis inoculation significantly altered the relative abundance of 18 genera at different time points (P < 0.05) with relative abundance significantly changed after 14 dpi. The abundance of those genera changed dramatically between 28 and 35 dpi in the treated group compared to control group. Positive correlations existed between Bacillus and Blautia and between Coprococcus and Flavonifractor following S. Enteritidis inoculation.

Conclusions

Our results indicated that both development and S. Enteritidis have effect on chicken cecal microbiota profiles. S. Enteritidis inoculation in young chicks influences the cecal microbiota mainly at 7 and 14 dpi. The cecal microbiota exhibited immunity to S. Enteritidis inoculation at 28 dpi. These findings will provide basic knowledge of the role that chicken cecal microbiota play in response to S. Enteritidis inoculation.
Appendix
Available only for authorised users
Literature
1.
Karaffová V, Bobíková K, Husáková E, Levkut M, Herich R, Revajová V, Levkutová M, Levkut M. Interaction of TGF-β4 and IL-17 with IgA secretion in the intestine of chickens fed with E. faecium AL41 and challenged with S. Enteritidis. Res Vet Sci. 2015;100:75–9.CrossRefPubMed
2.
3.
Qu A, Brulc JM, Wilson MK, Law BF, Theoret JR, Joens LA, Konkel ME, Angly F, Dinsdale EA, Edwards RA. Comparative metagenomics reveals host specific metavirulomes and horizontal gene transfer elements in the chicken cecum microbiome. PLoS ONE. 2008;3(8):e2945.CrossRefPubMedPubMedCentral
4.
Sergeant MJ, Constantinidou C, Cogan TA, Bedford MR, Penn CW, Pallen MJ. Extensive microbial and functional diversity within the chicken cecal microbiome. PLoS ONE. 2014;9(3):e91941.CrossRefPubMedPubMedCentral
5.
Wei S, Morrison M, Yu Z. Bacterial census of poultry intestinal microbiome. Poult Sci. 2013;92(3):671–83.CrossRefPubMed
6.
Myszka M, Klinger M. The immunomodulatory role of Vitamin D. Postępy Higieny I Medycyny Doświadczalnej. 2014;68(871113):865–78.CrossRefPubMed
7.
Ballou AL, Ali RA, Mendoza MA, Ellis JC, Hassan HM, Croom WJ, Koci MD. Development of the chick microbiome: how early exposure influences future microbial diversity. Front Vet Sci. 2016;3:2.CrossRefPubMedPubMedCentral
8.
Sekelja M, Rud I, Knutsen SH, Denstadli V, Westereng B, Næs T, Rudi K. Abrupt temporal fluctuations in the chicken fecal microbiota are explained by its gastrointestinal origin. Appl Environ Microbiol. 2012;78(8):2941–8.CrossRefPubMedPubMedCentral
9.
Wang L, Zhang J, Ma C, Sun Z, Fu W, Zhang H. Gut microbiota of chickens: a review. Chin J Anim Nutr. 2013; 25(3): 494–502.
10.
Zhu XY, Zhong T, Pandya Y, Joerger RD. 16S rRNA-based analysis of microbiota from the cecum of broiler chickens. Appl Environ Microbiol. 2002;68(1):124.CrossRefPubMedPubMedCentral
11.
12.
Mon KK, Saelao P, Halstead MM, Chanthavixay G, Chang HC, Garas L, Maga EA, Zhou H. Salmonella enterica serovars Enteritidis infection alters the indigenous microbiota diversity in young layer chicks. Front Vet Sci. 2015;2:61.CrossRefPubMedPubMedCentral
13.
Borewicz KA, Kim HB, Singer RS, Gebhart CJ, Sreevatsan S, Johnson T, Isaacson RE. Changes in the porcine intestinal microbiome in response to infection with Salmonella enterica and Lawsonia intracellularis. PLoS ONE. 2015;10(10):e0139106.CrossRefPubMedPubMedCentral
14.
Oakley BB, Kogut MH. Spatial and temporal changes in the broiler chicken cecal and fecal microbiomes and correlations of bacterial taxa with cytokine gene expression. Front Vet Sci. 2016;3:11.CrossRefPubMedPubMedCentral
15.
Petra V, Frantisek S, Hana H, Marcela F, Ivan R. Influence of Salmonella enterica serovar Enteritidis infection on the composition of chicken cecal microbiota. BMC Vet Res. 2013;9(1):140.CrossRef
16.
Thibodeau A, Fravalo P, Yergeau É, Arsenault J, Lahaye L, Letellier A. Chicken caecal microbiome modifications induced by Campylobacter jejuni colonization and by a non-antibiotic feed additive. PLoS ONE. 2015;10(7):e0131978.CrossRefPubMedPubMedCentral
17.
Guo H, Shao Y, Menghe B, Zhang H. Research on the relation between gastrointestinal microbiota and disease. Microbiol China. 2015;42:400–10.
18.
Kodama Y, Shumway M, Leinonen R. International nucleotide sequence database C: the sequence read archive: explosive growth of sequencing data. Nucleic Acids Res. 2012;40(Database issue):D54–6.CrossRefPubMed
19.
Leinonen R, Sugawara H, Shumway M. International nucleotide sequence database C: the sequence read archive. Nucleic Acids Res. 2011;39(Database issue):D19–21.CrossRefPubMed
20.
21.
22.
Edgar RC, Haas BJ, Clemente JC, Quince C, Knight R. UCHIME improves sensitivity and speed of chimera detection. Bioinformatics. 2011;27(16):2194–200.CrossRefPubMedPubMedCentral
23.
Edgar RC. Search and clustering orders of magnitude faster than BLAST. Bioinformatics. 2010;26(19):2460.CrossRefPubMed
24.
Bokulich NA, Subramanian S, Faith JJ, Gevers D, Gordon JI, Knight R, Mills DA, Caporaso JG. Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing. Nat Methods. 2013;10(1):57.CrossRefPubMed
25.
Schloss PD, Westcott SL, Ryabin T, Hall JR, Hartmann M, Hollister EB, Lesniewski RA, Oakley BB, Parks DH, Robinson CJ. Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl Environ Microbiol. 2009;75(23):7537.CrossRefPubMedPubMedCentral
26.
27.
Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol. 2016;33(7):1870–4.CrossRefPubMed
28.
Yeoman CJ, Chia N, Yildirim S, Miller ME, Kent A, Stumpf R, Leigh SR, Nelson KE, White BA, Wilson BA. Towards an evolutionary model of animal-associated microbiomes. Entropy. 2011;13(3):570–94.CrossRef
29.
Yeoman CJ, Chia N, Jeraldo P, Sipos M, Goldenfeld ND, White BA. The microbiome of the chicken gastrointestinal tract. Anim Health Res Rev. 2012;13(1):89–99.CrossRefPubMed
30.
Sommer F, Backhed F. The gut microbiota–masters of host development and physiology. Nat Rev Microbiol. 2013;11(4):227–38.CrossRefPubMed
31.
Bremermann HJ, Thieme HR. A competitive exclusion principle for pathogen virulence. J Math Biol. 1989;27(2):179–90.CrossRefPubMed
32.
Cisek AA, Binek M. Chicken intestinal microbiota function with a special emphasis on the role of probiotic bacteria. Pol J Vet Sci. 2014;17(2):385–94.CrossRefPubMed
33.
Nordentoft S, Mølbak L, Bjerrum L, Vylder JD, Immerseel FV, Pedersen K. The influence of the cage system and colonisation of Salmonella Enteritidis on the microbial gut flora of laying hens studied by T-RFLP and 454 pyrosequencing. BMC Microbiol. 2011;11(1):187.CrossRefPubMedPubMedCentral
34.
Croswell A, Amir E, Teggatz P, Barman M, Salzman NH. Prolonged impact of antibiotics on intestinal microbial ecology and susceptibility to enteric Salmonella infection. Infect Immun. 2009;77(7):2741.CrossRefPubMedPubMedCentral
35.
Dominguezbello MG, Blaser MJ, Ley RE, Knight R. Development of the human gastrointestinal microbiota and insights from high-throughput sequencing. Gastroenterology. 2011;140(6):1713.CrossRef
36.
Jakobsson HE, Jernberg C, Andersson AF, Sjölundkarlsson M, Jansson JK, Engstrand L. Short-term antibiotic treatment has differing long-term impacts on the human throat and gut microbiome. PLoS ONE. 2010;5(3):e9836.CrossRefPubMedPubMedCentral
37.
Bedford RB, Draper SM, Scully PN, Welch SL. ChemInform abstract: palladium bis(phosphinite) “PCP”-pincer complexes and their application as catalysts in the Suzuki reaction. New J Chem. 2000;24(10):745–7.CrossRef
38.
Pourabedin M, Zhao X. Prebiotics and gut microbiota in chickens. FEMS Microbiol Lett. 2015;362(15):fnv122.CrossRefPubMed
39.
Barrow PA, Smith HW, Tucker JF. The effect of feeding diets containing avoparcin on the excretion of salmonellas by chickens experimentally infected with natural sources of Salmonella organisms. Epidemiol Infect. 1989;102(2):239–52.CrossRefPubMedPubMedCentral
Metadata
Title
Cecal microbiome profile altered by Salmonella enterica, serovar Enteritidis inoculation in chicken
Authors
Liying Liu
Lili Lin
Linna Zheng
Hui Tang
Xinzhong Fan
Nianguo Xue
Min Li
Min Liu
Xianyao Li
Publication date
01-12-2018
Publisher
BioMed Central
Published in
Gut Pathogens / Issue 1/2018
Electronic ISSN: 1757-4749
DOI
https://doi.org/10.1186/s13099-018-0261-x

Other articles of this Issue 1/2018

Gut Pathogens 1/2018 Go to the issue

Research

Antimicrobial resistance in Enterobacteriaceae from healthy broilers in Egypt: emergence of colistin-resistant and extended-spectrum β-lactamase-producing Escherichia coli

Research

Genomic characterization of malonate positive Cronobacter sakazakii serotype O:2, sequence type 64 strains, isolated from clinical, food, and environment samples

Letter to the Editor

Antimicrobial resistant Helicobacter fennelliae isolated from non-diarrheal child stool sample in Battambang, Cambodia

Research

Identification of a new alanine racemase in Salmonella Enteritidis and its contribution to pathogenesis

Research

Impact of the introduction of a nucleic acid amplification test for Clostridium difficile diagnosis on stool rejection policies

Research

Genome divergence and increased virulence of outbreak associated Salmonella enterica subspecies enterica serovar Heidelberg
Obesity Clinical Trial Summary

At a glance: The STEP trials

Read more

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine

Highlights from the ACC 2024 Congress

Year in Review: Pediatric cardiology

Pediatric Cardiology Video

Watch Dr. Anne Marie Valente present the last year's highlights in pediatric and congenital heart disease in the official ACC.24 Year in Review session.

Year in Review: Pulmonary vascular disease

Cardiopulmonary Comorbidity Video

The last year's highlights in pulmonary vascular disease are presented by Dr. Jane Leopold in this official video from ACC.24.

Year in Review: Valvular heart disease

Valvular Heart Disease Video

Watch Prof. William Zoghbi present the last year's highlights in valvular heart disease from the official ACC.24 Year in Review session.

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discuss last year's major advances in heart failure and cardiomyopathies.

ACC 2024 Congress Coverage

Heart Failure Video

Year in Review: Heart failure and cardiomyopathies

Watch now

Watch this official video from ACC.24. Dr. Biykem Bozkurt discuss last year's major advances in heart failure and cardiomyopathies.

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

16-04-2024 Type 2 Diabetes News

Incentivised to exercise

11-04-2024 Lifestyle Modifications News

New intervention for STEMI-related cardiogenic shock

10-04-2024 Myocardial Infarction News

» View more highlights on the ACC 2024 congress hub page

Image Credits