Top

Gut Pathogens

Published in:

Open Access 01-12-2017 | Short report

Gut dysbiosis in mice genetically selected for low antibody production

Authors: Ana Carolina da Silva Santos, José Ricardo Jensen, Silvio Luis de Oliveira, Josias Rodrigues

Published in: Gut Pathogens | Issue 1/2017

Abstract

Background

Dysbiosis is linked to the cause of several human diseases, many of which having an immunity related component. This work investigated whether mice genetically selected for low or high antibody production display differences in intestinal bacterial communities, and consisted in the comparison of fecal 16SV6–V8 rDNA PCR amplicons resolved by temperature gradient gel electrophoresis (TGGE) of five each of low (LIII) and high (HIII) antibody producing mice. 16SV6 rDNA amplicons of 2 mice from each line were sequenced.

Results

LIII mice were grouped in a single TGGE cluster, displayed a low α-diversity, and were distinguished by low Firmicutes/Bacteroidetes ratio.

Conclusion

The results suggest that genetically driven low antibody production in mice is associated with gut dysbiosis.

Belkaid Y, Hand TW. Role of the microbiota in immunity and inflammation. Cell. 2014;157:121–41.CrossRefPubMedPubMedCentral

Sant’Anna OA, Bouthillier Y, Mevel JC, de Franco M, Mouton D. Isotypic distribution of antibody responses in lines of mice selected for high or low immunoresponsiveness. Braz J Med Biol Res. 1991;24:407–16.PubMed

Biozzi G, Mouton D, Sant’Anna OA, Passos HC, Gennari M, Reis MH, et al. Genetics of immunoresponsiveness to natural antigens in the mouse. Curr Top Microbiol Immunol. 1979;85:31–98.PubMed

Ibanez OM, Mouton D, Ribeiro OG, Bouthillier Y, De Franco M, Cabrera WH, et al. Low antibody responsiveness is found to be associated with resistance to chemical skin tumorigenesis in several lines of Biozzi mice. Cancer Lett. 1999;136:153–8.CrossRefPubMed

Sant’Anna OA, Massa S, Mouton D, Bouthillier Y, Mevel JC, Ibanez OM, et al. Salmonella typhimurium infection in high and low antibody responder mice: inverse correlation between antibody responsiveness and resistance to infection. FEMS Microbiol Immunol. 1989;1:465–71.CrossRefPubMed

Jensen JR, Peters LC, Borrego A, Ribeiro OG, Cabrera WH, Starobinas N, et al. Involvement of antibody production quantitative trait loci in the susceptibility to pristane-induced arthritis in the mouse. Genes Immun. 2006;7:44–50.CrossRefPubMed

Baldon EJ, Marengo EB, de Franco M, Starobinas N, Bueno V, Sant’anna OA. Mycobacterium leprae Hsp65 administration reduces the lifespan of aged high antibody producer mice. Immun Ageing. 2014;11:6.CrossRefPubMedPubMedCentral

Vorraro F, Cabrera WH, Ribeiro OG, Jensen JR, De Franco M, Ibanez OM, et al. Trypanosoma cruzi infection in genetically selected mouse lines: genetic linkage with quantitative trait locus controlling antibody response. Mediators Inflamm. 2014;2014:952857.CrossRefPubMedPubMedCentral

Siqueira M, Bandieri A, Reis MS, Sant’anna OA, Biozzi G. Selective breeding of mice for antibody responsiveness to flagellar and somatic antigens of salmonellae. Eur J Immunol. 1976;6:241–9.CrossRefPubMed

10.

Hooper LV, Littman DR, Macpherson AJ. Interactions between the microbiota and the immune system. Science. 2012;336:1268–73.CrossRefPubMedPubMedCentral

11.

Zoetendal EG, Akkermans AD, De Vos WM. Temperature gradient gel electrophoresis analysis of 16S rRNA from human fecal samples reveals stable and host-specific communities of active bacteria. Appl Environ Microbiol. 1998;64:3854–9.PubMedPubMedCentral

12.

Andersson AF, Lindberg M, Jakobsson H, Backhed F, Nyren P, Engstrand L. Comparative analysis of human gut microbiota by barcoded pyrosequencing. PLoS ONE. 2008;3:e2836.CrossRefPubMedPubMedCentral

13.

Garcia-Vallve S, Palau J, Romeu A. Horizontal gene transfer in glycosyl hydrolases inferred from codon usage in Escherichia coli and Bacillus subtilis. Mol Biol Evol. 1999;16:1125–34.CrossRefPubMed

14.

Meyer F, Paarmann D, D’Souza M, Olson R, Glass EM, Kubal M, et al. The metagenomics RAST server—a public resource for the automatic phylogenetic and functional analysis of metagenomes. BMC Bioinformatics. 2008;9:386.CrossRefPubMedPubMedCentral

15.

Cole JR, Wang Q, Fish JA, Chai B, McGarrell DM, Sun Y, et al. Ribosomal Database Project: data and tools for high throughput rRNA analysis. Nucleic Acids Res. 2014;42:D633–42.CrossRefPubMed

16.

De Carvalho VR, da Silva Santos AC, Rodrigues J. Temperature gradient gel electrophoresis as a valuable accessory tool for assessment of dysbiosis in Crohn’s disease. Adv Microbiol. 2016;6:549–54.CrossRef

17.

Schubert AM, Rogers MA, Ring C, Mogle J, Petrosino JP, Young VB, et al. Microbiome data distinguish patients with Clostridium difficile infection and non-C. difficile-associated diarrhea from healthy controls. MBio. 2014;5:e01021–2014.CrossRefPubMedPubMedCentral

18.

Ye J, Lee JW, Presley LL, Bent E, Wei B, Braun J, et al. Bacteria and bacterial rRNA genes associated with the development of colitis in IL-10 (−/−) mice. Inflamm Bowel Dis. 2008;14:1041–50.CrossRefPubMedPubMedCentral

19.

Takahashi K, Nishida A, Fujimoto T, Fujii M, Shioya M, Imaeda H, et al. Reduced abundance of butyrate-producing bacteria species in the fecal microbial community in Crohn’s disease. Digestion. 2016;93:59–65.CrossRefPubMed

20.

Atarashi K, Tanoue T, Oshima K, Suda W, Nagano Y, Nishikawa H, et al. Treg induction by a rationally selected mixture of Clostridia strains from the human microbiota. Nature. 2013;500:232–6.CrossRefPubMed

21.

Xu X, Zhang X. Effects of cyclophosphamide on immune system and gut microbiota in mice. Microbiol Res. 2015;171:97–106.CrossRefPubMed

22.

Elinav E, Strowig T, Kau AL, Henao-Mejia J, Thaiss CA, Booth CJ, et al. NLRP6 inflammasome regulates colonic microbial ecology and risk for colitis. Cell. 2011;145:745–57.CrossRefPubMedPubMedCentral

23.

Lucke K, Miehlke S, Jacobs E, Schuppler M. Prevalence of Bacteroides and Prevotella spp. in ulcerative colitis. J Med Microbiol. 2006;55:617–24.CrossRefPubMed

24.

Hildebrand F, Nguyen TL, Brinkman B, Yunta RG, Cauwe B, Vandenabeele P, et al. Inflammation-associated enterotypes, host genotype, cage and inter-individual effects drive gut microbiota variation in common laboratory mice. Genome Biol. 2013;14:R4.CrossRefPubMedPubMedCentral

Title: Gut dysbiosis in mice genetically selected for low antibody production
Authors: Ana Carolina da Silva Santos
José Ricardo Jensen
Silvio Luis de Oliveira
Josias Rodrigues
Publication date: 01-12-2017
Publisher: BioMed Central
Published in: Gut Pathogens / Issue 1/2017
Electronic ISSN: 1757-4749
DOI: https://doi.org/10.1186/s13099-017-0193-x

ACC 2024 Congress Coverage

Heart Failure Video

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Gut dysbiosis in mice genetically selected for low antibody production

Abstract

Background

Results

Conclusion

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Background

Results

Conclusion

Please log in to get access to this content

Other articles of this Issue 1/2017

Recovery of Salmonella isolated from eggs and the commercial layer farms

Class 1 integrons and plasmid-mediated multiple resistance genes of the Campylobacter species from pediatric patient of a university hospital in Taiwan

Helicobacter pylori virulence genes of minor ethnic groups in North Thailand

Genotypic diversity, antimicrobial resistance and biofilm-forming abilities of Campylobacter isolated from chicken in Central China

Existence of a novel qepA variant in quinolone resistant Escherichia coli from aquatic habitats of Bangladesh

Strain-specific probiotic properties of lactic acid bacteria and their interference with human intestinal pathogens invasion

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page