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Arthritis Research & Therapy
Published in: Arthritis Research & Therapy 1/2016

Open Access 01-12-2016 | Research article

Long noncoding RNA expression profile in fibroblast-like synoviocytes from patients with rheumatoid arthritis

Authors: Yu Zhang, Yu-Zhong Xu, Ning Sun, Jian-Hong Liu, Fang-Fang Chen, Xiao-Long Guan, Ang Li, Fei Wang, Qin-Fei Zhao, Hai-Yong Wang, Shu-Sheng Song, Wei Yu, Jian-Ning Zhao, Xiao-Jun Li

Published in: Arthritis Research & Therapy | Issue 1/2016

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Abstract

Background

Long noncoding RNAs (lncRNAs) have recently received wide attention as key molecules that mediate a variety of physiological and pathological processes by regulating gene expression; however, knowledge of lncRNAs in rheumatoid arthritis (RA) is limited. Thus, we investigated the lncRNA expression profile in fibroblast-like synoviocytes (FLSs) from patients with RA and explored the function of abundantly expressed lncRNAs.

Methods

LncRNA and mRNA microarrays were performed to identify differentially expressed lncRNAs in RA FLSs compared with normal FLSs. Quantitative polymerase chain reaction (qPCR) was used to validate the results, and correlation analysis was used to analyze the relationship between these aberrantly expressed lncRNAs and clinical characteristics. A receiver operating characteristic (ROC) curve was constructed to evaluate the diagnostic value of the lncRNAs identified.

Results

According to the gene expression profiles, 135 lncRNAs were differentially expressed between RA and normal FLSs. Furthermore, qPCR data showed that lncRNA ENST00000483588 was up-regulated and that three lncRNAs (ENST00000438399, uc004afb.1, and ENST00000452247) were down-regulated in RA FLSs. The expression level of ENST00000483588 was positively correlated with the level of C-reactive protein and the Simplified Disease Activity Index score. Moreover, the areas under the ROC curve were 0.85, 0.92, 0.97, and 0.92 for ENST00000483588, ENST00000438399, uc004afb.1, and ENST00000452247, respectively.

Conclusions

The results indicate that the dysregulation of ENST00000483588, ENST00000438399, uc004afb.1, and ENST00000452247 may be involved in the pathological processes of RA and that these lncRNAs may have potential value for the diagnosis and assessment of the disease activity of RA.
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Metadata
Title
Long noncoding RNA expression profile in fibroblast-like synoviocytes from patients with rheumatoid arthritis
Authors
Yu Zhang
Yu-Zhong Xu
Ning Sun
Jian-Hong Liu
Fang-Fang Chen
Xiao-Long Guan
Ang Li
Fei Wang
Qin-Fei Zhao
Hai-Yong Wang
Shu-Sheng Song
Wei Yu
Jian-Ning Zhao
Xiao-Jun Li
Publication date
01-12-2016
Publisher
BioMed Central
Published in
Arthritis Research & Therapy / Issue 1/2016
Electronic ISSN: 1478-6362
DOI
https://doi.org/10.1186/s13075-016-1129-4

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