Top

Published in:

Open Access 01-12-2019 | Denture | Study protocol

Hygiene protocols for the treatment of denture-related stomatitis: local and systemic parameters analysis - a randomized, double-blind trial protocol

Authors: Adriana B. Ribeiro, Camila B. de Araújo, Luiz Eduardo V. Silva, Rubens Fazan-Junior, Helio C. Salgado, Aline B. Ribeiro, Caroline V. Fortes, Frank L. Bueno, Viviane C. de Oliveira, Helena de F. O. Paranhos, Evandro Watanabe, Cláudia H. da Silva-Lovato

Published in: Trials | Issue 1/2019

Abstract

Background

Denture-related stomatitis (DS) is chronic multifactorial inflammation, strongly related to the presence of the biofilm that is the complex structure formed by microorganisms held together by a mucus-like matrix of carbohydrate that adheres to different surfaces, including the denture surface. DS has recently been correlated with deleterious cardiovascular alterations. The potential effect of hygiene protocols in the control of DS and randomized clinical trials that address this oral condition with cardiovascular complications are important in clinical decision-making.

Material/design

A clinical trial, randomized, double-blind, and with parallel groups, will be conducted in Brazil The sample will consist of 100 patients without teeth in both arches, users of at least maxillary complete dentures, and diagnosed with DS, who will be allocated to groups (n = 25 per group) according to the different hygiene protocols: (1) brushing of the palate and immersion of the prosthesis in 0.25% sodium hypochlorite solution (positive control); (2) brushing of the palate and immersion of the prosthesis in 0.15% triclosan solution; (3) brushing of the palate and immersion of the prosthesis in lactose monohydrate; or (4) brushing the palate with citric acid and immersing the prosthesis in lactose monohydrate. The response variables will be heart rate variability and alteration of blood pressure (systemic level), remission of DS, removal of biofilm, reduction of microbial load (colony-forming units (CFU)), mouth and prosthesis odor level, expression of MUC1, proinflammatory cytokines, C-reactive protein (CRP), viscosity, pH and salivary flow (locally); patient-centred qualitative analysis will also be undertaken. Measurements will be performed at baseline and 10 days after the interventions. The results obtained will be statistically analyzed as pertinent, with a level of significance of 0.05.

Discussion

This study will provide a guideline for clinical practice regarding the use of hygiene protocols in the treatment of oral diseases (DS) mediated by biofilm. Also, it may provide evidence of correlation of oral manifestation with cardiac risk.

Trial registration

Brazilian Registry of Clinical Trials, RBR-4hhwjb. Registered on 9 November 2018.

Available only for authorised users

Hannah VE, O’Donnell L, Robertson D, Ramage G. Denture stomatitis: causes, cures and prevention. Prim Dent J. 2017;6(4):46–51. https://doi.org/10.1308/205016817822230175.CrossRefPubMed

Kulak-Ozkan Y, Kazazoglu E, Arikan A. Oral hygiene habits, denture cleanliness, presence of yeasts and stomatitis in elderly people. J Oral Rehabil. 2002;29(3):300–4.CrossRef

Regis RR, Cunha TR, Della Vecchia MP, Ribeiro AB, Silva-Lovato CH, de Souza RF. A randomised trial of a simplified method for complete denture fabrication: patient perception and quality. J Oral Rehabil. 2013. https://doi.org/10.1111/joor.12063.

Campos MS, Marchini L, Bernardes LAS, Paulino LC, Nobrega FG. Biofilm microbial communities of denture stomatitis. Oral Microbiol Immunol. 2008. https://doi.org/10.1111/j.1399-302X.2008.00445.x.

Ghannoum MA, Jurevic RJ, Mukherjee PK, Cui F, Sikaroodi M, Naqvi A, et al. Characterization of the oral fungal microbiome (mycobiome) in healthy individuals. PLoS Pathog. 2010. https://doi.org/10.1371/journal.ppat.1000713.

Gasparoto TH, Sipert CR, de Oliveira CE, Porto VC, Santos CF, Campanelli AP, et al. Salivary immunity in elderly individuals presented with Candida-related denture stomatitis. Gerodontology. 2012. https://doi.org/10.1111/j.1741-2358.2011.00476.x.

Garrett NR. Poor oral hygiene, wearing dentures at night, perceptions of mouth dryness and burning, and lower educational level may be related to oral malodor in denture wearers. J Evid Based Dent Pract. 2010. https://doi.org/10.1016/j.jebdp.2009.11.026.

Gendreau L, Loewy ZG. Epidemiology and Etiology of Denture Stomatitis. J Prosthodont. 2011. https://doi.org/10.1111/j.1532-849X.2011.00698.x.

Kossioni AE. The prevalence of denture stomatitis and its predisposing conditions in an older Greek population. Gerodontology. 2011. https://doi.org/10.1111/j.1741-2358.2009.00359.x.

10.

Kabawat M, de Souza RF, Badaró MM, de Koninck L, Barbeau J, Rompré P, Emami E. Phase 1 clinical trial on the effect of palatal brushing on denture stomatitis. Int J Prosthodont. 2014. https://doi.org/10.11607/ijp.3844.

11.

Badaró MM, Salles MM, Leite VMF, Arruda CNF, Oliveira VC, Nascimento C, Souza RF, Paranhos HFO, Silva-Lovato CH. Clinical trial for evaluation of Ricinus communis and sodium hypochlorite as denture cleanser. J Appl Oral Sci. 2017a. https://doi.org/10.1590/1678-7757-2016-0222.

12.

Felipe LO, Júnior WFDS, Araújo KC, Fabrino DL. Lactoferrin, chitosan and Melaleuca alternifolia-natural products that show promise in candidiasis treatment. Braz J Microbiol. 2018. https://doi.org/10.1016/j.bjm.2017.05.008.

13.

Abaci O, Haliki-Uztan A, Ozturk B, Toksavul S, Ulusoy M, Boyacioglu H. Determining Candida spp. incidence in denture wearers. Mycopathologia. 2010; doi: https://doi.org/10.1007/s11046-010-9275-8.

14.

Salerno C, Pascale M, Contaldo M, Esposito V, Busciolano M, Milillo L, et al. Candida-associated denture stomatitis. Med Oral Patol Oral Cir Bucal. 2011;16:e139–43.CrossRef

15.

Bars P, Kouadio AA, N’Goran JK, Badran Z, Soueidan A. Relationship between removable prosthesis and some systemics disorders. J Indian Prosthodont Soc. 2015. https://doi.org/10.4103/0972-4052.171828.

16.

Maciąg J, Mikołajczyk T, Matusik P, Nowakowski D, Robertson D, Maciąg A, Osmenda G, Cześnikiewicz-Guzik M. The effect of treatment of denture-related stomatitis on peripheral T cells and monocytes. Oral Health Prev Dent. 2017;15(3):259–68. https://doi.org/10.3290/j.ohpd.a38527.CrossRefPubMed

17.

Osmenda G, Maciąg J, Wilk G, Maciąg A, Nowakowski D, Loster J, Dembowska E, Robertson D, Guzik T, Cześnikiewicz-Guzik M. Treatment of denture-related stomatitis improves endothelial function assessed by flow-mediated vascular dilation. Arch Med Sci. 2017. https://doi.org/10.5114/aoms.2017.64715.

18.

Maciag J, Osmenda G, Nowakowski D, Wilk G, Maciag A, Mikolajczyk T, Nosalski R, Sagan A, Filip M, Dróżdż M, Loster J, Guzik TJ, Cześnikiewicz-Guzik M. Denture-related stomatitis is associated with endothelial dysfunction. Biomed Res Int. 2014. https://doi.org/10.1155/2014/474016.

19.

Chang WI, Chang JY, Kim YY, Lee G, Kho HS. MUC1 expression in the oral mucosal epithelial cells of the elderly. Arch Oral Biol. 2011. https://doi.org/10.1016/j.archoralbio.2011.02.007.

20.

Kho HS. Oral epithelial MUC1 and oral health. Oral Dis. 2018. https://doi.org/10.1111/odi.12713.

21.

Kang JH, Kim YY, Chang JY, Kho HS. Relationships between oral MUC1 expression and salivary hormones in burning mouth syndrome. Arch Oral Biol. 2017. https://doi.org/10.1016/j.archoralbio.2017.01.022.

22.

Gabryel-Porowska H, Gornowicz A, Bielawska A, Wójcicka A, Maciorkowska E, Grabowska SZ, Bielawski K. Mucin levels in saliva of adolescents with dental caries. Med Sci Monit. 2014;20:72–7. https://doi.org/10.12659/MSM.889718.CrossRefPubMedPubMedCentral

23.

Arimatsu K, Yamada H, Miyazawa H, et al. Oral pathobiont induces systemic inflammation and metabolic changes associated with alteration of gut microbiota. Sci Rep. 2014;4:4828. https://doi.org/10.1038/srep04828.CrossRefPubMedPubMedCentral

24.

Preshaw PM, Taylor JJ. How has research into cytokine interactions and their role in driving immune responses impacted our understanding of periodontitis? J Clin Periodontol. 2011. https://doi.org/10.1111/j.1600-051X.2010.01671.x.

25.

Aghdasi-Bornaun H, Kutluk G, Keskindemirci G, Öztarhan K, Dedeoğlu R, Yılmaz N, Tosun Ö. Evaluation of autonomic nervous system functions in frame of heart rate variability in children with inflammatory bowel disease in remission. Turk J Pediatr. 2018;60(4):407–14. https://doi.org/10.24953/turkjped.2018.04.008.CrossRefPubMed

26.

Rosas-Ballina M, Olofsson PS, Ochani M, Valdés-Ferrer SI, Levine YA, Reardon C, Tusche MW, Pavlov VA, Andersson U, Chavan S, et al. Acetylcholine-synthesizing T cells relay neural signals in a vagus nerve circuit. Science. 2011. https://doi.org/10.1126/science.1209985.

27.

Gidron Y, Deschepper R, De Couck M, Thayer JF, Velkeniers B. The vagus nerve can predict and possibly modulate non-communicable chronic diseases: introducing a neuroimmunological paradigm to public health. J Clin Med. 2018. https://doi.org/10.3390/jcm7100371.

28.

Anastassiadou V, Naka O, Heath MR, Kapari D. Validation of índices for funtional assessment of dentures. Gerodontology. 2002;19:46–52.CrossRef

29.

de Souza RF, Khiyani MF, Chaves CAL, Feine J, Barbeau J, Fuentes R, Borie E, Crizostomo LC, Silva-Lovato CH, Rompre P, Emami E. Improving practice guidelines for the treatment of denture-related erythematous stomatitis: a study protocol for a randomized controlled trial. Trials. 2017. https://doi.org/10.1186/s13063-017-1947-y.

30.

Pedrazzi V, Sato S, de Mattos Mda G, Lara EH, Panzeri H. Tongue-cleaning methods: a comparative clinical trial employing a toothbrush and a tongue scraper. J Periodontol. 2004;75(7):1009–12.CrossRef

31.

Shekhar A, Das S, Bhattacharyya J, Goel P, Majumdar S, Ghosh S. A comparative analysis of salivary factors and maxillary denture retention in different arch forms: an in vivo study. J Indian Prosthodont Soc. 2018;18(1):53–60. https://doi.org/10.4103/jips.jips_110_17.CrossRefPubMedPubMedCentral

32.

Ramírez-Amador V, Zambrano JG, Anaya-Saavedra G, Zentella-Dehesa A, Irigoyen-Camacho E, Meráz-Cruz N, Ponce de León-Rosales S. TNF as marker of oral candidiasis, HSV infection, and mucositis onset during chemotherapy in leukemia patients. Oral Dis. 2017;23(7):941–8. https://doi.org/10.1111/odi.12677.CrossRefPubMed

33.

Justino CI, Duarte K, Lucas S, Chaves P, Bettencourt P, Freitas AC, Pereira R, Cardoso S, Duarte AC, Rocha-Santos TA. Assessment of cardiovascular disease risk using immunosensors for determination of C-reactive protein levels in serum and saliva: a pilot study. Bioanalysis. 2014. https://doi.org/10.4155/bio.14.12.

34.

Souza RF, Patrocínio L, Pero AC, Marra J, Compagnoni MA. Reliability and validation of a Brazilian version of the Oral Health Impact Profile for assessing edentulous subjects. J Oral Rehabil. 2007;34(11):821–6.CrossRef

Title: Hygiene protocols for the treatment of denture-related stomatitis: local and systemic parameters analysis - a randomized, double-blind trial protocol
Authors: Adriana B. Ribeiro
Camila B. de Araújo
Luiz Eduardo V. Silva
Rubens Fazan-Junior
Helio C. Salgado
Aline B. Ribeiro
Caroline V. Fortes
Frank L. Bueno
Viviane C. de Oliveira
Helena de F. O. Paranhos
Evandro Watanabe
Cláudia H. da Silva-Lovato
Publication date: 01-12-2019
Publisher: BioMed Central
Keyword: Denture
Published in: Trials / Issue 1/2019
Electronic ISSN: 1745-6215
DOI: https://doi.org/10.1186/s13063-019-3854-x

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Hygiene protocols for the treatment of denture-related stomatitis: local and systemic parameters analysis - a randomized, double-blind trial protocol

Abstract

Background

Material/design

Discussion

Trial registration

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Background

Material/design

Discussion

Trial registration

Please log in to get access to this content

Other articles of this Issue 1/2019

Clinical efficacy of different marginal forms of endocrowns: study protocol for a randomized controlled trial

High-flow nasal therapy versus noninvasive ventilation in COPD patients with mild-to-moderate hypercapnic acute respiratory failure: study protocol for a noninferiority randomized clinical trial

Completeness of reporting in abstracts of randomized controlled trials in subscription and open access journals: cross-sectional study

Effects of C8 nerve root block during interscalene brachial plexus block on anesthesia of the posterior shoulder in patients undergoing arthroscopic shoulder surgery: study protocol for a prospective randomized parallel-group controlled trial

Study protocol for a randomized, blinded, controlled trial of ketamine for acute painful crisis of sickle cell disease

Polyherbal dietary supplementation for prediabetic adults: study protocol for a randomized controlled trial