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Open Access 01-12-2017 | Research

MicroRNA-205 promotes cell invasion by repressing TCF21 in human ovarian cancer

Authors: Jun Wei, Lahong Zhang, Jennifer Li, Shuguang Zhu, Minghui Tai, Clifford W. Mason, Julia A. Chapman, Evelyn A. Reynolds, Carl P Weiner, Helen H Zhou

Published in: Journal of Ovarian Research | Issue 1/2017

Abstract

Background

Ovarian cancer is the leading lethal, gynecological malignancy in the United States. No doubt, the continued morbidity and mortality of ovarian cancer reflects a poor understanding of invasive mechanisms. Recent studies reveal that ovarian cancers express aberrant microRNAs (miRNAs or miRs), some of which have oncogenic or tumor suppressor properties. Several studies suggested that miR-205 is involved in tumorigenesis. Presently, we investigate the molecular mechanisms and target of miR-205 in ovarian cancer.

Methods

Quantitative real-time polymerase chain reaction and western blot were performed to assess miR-205 and transcription factor 21 (TCF21) expression in ovarian cancer and normal ovary samples. The effect of miR-205 on TCF21 was determined by luciferase reporter assay and western blot. The effect of miR-205 and TCF21 on cell invasion was quantitated using transwell invasion assay.

Result

miR-205 expression was increased in ovarian cancer and it promoted the invasive behavior of ovarian cancer cell lines (OVCAR-5, OVCAR-8 and SKOV-3). miR-205 directly targeted TCF21, which was significantly decreased in ovarian cancer tissue. miR-205 inhibited TCF21 expression and as a consequence blunted the inhibitory effect of TCF21 on cell invasion. Matrix Metalloproteinases (MMPs) play an important role in cancer invasion and metastasis. TCF21 inhibited MMP-2 and MMP-10 and decreased ovarian cancer cell invasion. Co-transfection of TCF21 expression plasmid with miR-205 mimic diminished the inhibitory effect of TCF21 on MMP-2 and MMP-10 in ovarian cancer cells.

Conclusion

miR-205 appears to have an important role in the spread of ovarian cancer by targeting TCF21. These findings offer a new mechanism of ovarian cancer tumorigenesis, which could be useful for the development of new therapeutic approaches to ovarian cancer treatment.

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Title: MicroRNA-205 promotes cell invasion by repressing TCF21 in human ovarian cancer
Authors: Jun Wei
Lahong Zhang
Jennifer Li
Shuguang Zhu
Minghui Tai
Clifford W. Mason
Julia A. Chapman
Evelyn A. Reynolds
Carl P Weiner
Helen H Zhou
Publication date: 01-12-2017
Publisher: BioMed Central
Published in: Journal of Ovarian Research / Issue 1/2017
Electronic ISSN: 1757-2215
DOI: https://doi.org/10.1186/s13048-017-0328-1

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Springer Medicine

MicroRNA-205 promotes cell invasion by repressing TCF21 in human ovarian cancer

Abstract

Background

Methods

Result

Conclusion

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Background

Methods

Result

Conclusion

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