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Journal of Ovarian Research
Published in: Journal of Ovarian Research 1/2016

Open Access 01-12-2016 | Research

NPR2 is involved in FSH-mediated mouse oocyte meiotic resumption

Authors: Lei Yang, Qiang Wei, Wei Li, Qihui Xi, Xiaoe Zhao, Baohua Ma

Published in: Journal of Ovarian Research | Issue 1/2016

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Abstract

Background

Previous studies have reported that follicle-stimulating hormone (FSH) is often added to culture media to induce oocyte meiotic resumption and maturation and to improve subsequent embryonic development during in vitro maturation (IVM). However, the underlying mechanisms remain unclear.

Methods

Cumulus-oocyte complexes (COCs) were collected from ovaries 46–48 h after the female mice were intraperitoneally injected with 8 IU equine chorionic gonadotropin (eCG) and then the COCs were cultured in different medium. qRT-PCR analysis was used to assess mRNA expression of EGF-like factors and natriuretic peptide receptor 2 (NPR2). Western Blot analysis was used to assess phosphorylation of mitogen-activated protein kinase 3/1 (MAPK3/1). The oocytes were morphologically assessed for meiotic resumption.

Results

FSH stimulated the expression of EGF-like factors, the activation of MAPK3/1, a decrease in NPR2 mRNA and oocyte meiotic resumption. Moreover, the FSH-induced decrease in NPR2 and oocyte meiotic resumption occurred via the MAPK3/1 singling pathway, which was activated by the epidermal growth factor receptor (EGFR) pathway.

Conclusions

NPR2 is involved in FSH-mediated oocyte meiotic resumption, and this process is associated with the EGFR and MAPK3/1 signaling pathways.
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Metadata
Title
NPR2 is involved in FSH-mediated mouse oocyte meiotic resumption
Authors
Lei Yang
Qiang Wei
Wei Li
Qihui Xi
Xiaoe Zhao
Baohua Ma
Publication date
01-12-2016
Publisher
BioMed Central
Published in
Journal of Ovarian Research / Issue 1/2016
Electronic ISSN: 1757-2215
DOI
https://doi.org/10.1186/s13048-016-0218-y

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