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Open Access 01-12-2014 | Research

Anti Müllerian Hormone (AMH) level and expression in mural and cumulus cells in relation to age

Authors: Alon Kedem, Yuval Yung, Gil M Yerushalmi, Jigal Haas, Ettie Maman, Mirit Hanochi, Rina Hemi, Raoul Orvieto, Jehoshua Dor, Ariel Hourvitz

Published in: Journal of Ovarian Research | Issue 1/2014

Abstract

Background

Serum AMH is declining with age and is highly associated with ovarian follicular reserve and disordered folliculogenesis. However, the precise role of AMH in the process of human follicular aging has still to be determined.

Aim

This study investigates AMH level in the follicular fluid (FF) and mRNA expression pattern in cumulus and mural granulosa cells of human ovarian follicles in relation to age.

Methods

We conducted a prospective study. Sixty-eight women undergoing In vitro fertilization (IVF) treatment were enrolled in the study. We obtained FF, mural and cumulus granulosa cells from large preovulatory follicles (17-20 mm) of 21–35 years old women (n = 40) and 40–45 years old women (n = 28) during oocyte pickup.

Results

Higher level of AMH mRNA expression in cumulus cells was observed in the older age group compared to the younger (P <0.01). In accordance with AMH mRNA expression results, FF AMH protein levels were significantly higher in the older group than in the younger group (4.7 ± 1.1 ng\ml and 2.3 ± 0.2 ng\ml respectively, p < 0.002).

Conclusions

AMH is highly expressed and secreted from cumulus GCs of advanced age patients. This remarkable correlation between AMH mRNA levels in cumulus cells in respect to age suggests that AMH may be involved in follicular aging process.

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Title: Anti Müllerian Hormone (AMH) level and expression in mural and cumulus cells in relation to age
Authors: Alon Kedem
Yuval Yung
Gil M Yerushalmi
Jigal Haas
Ettie Maman
Mirit Hanochi
Rina Hemi
Raoul Orvieto
Jehoshua Dor
Ariel Hourvitz
Publication date: 01-12-2014
Publisher: BioMed Central
Published in: Journal of Ovarian Research / Issue 1/2014
Electronic ISSN: 1757-2215
DOI: https://doi.org/10.1186/s13048-014-0113-3

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Anti Müllerian Hormone (AMH) level and expression in mural and cumulus cells in relation to age

Abstract

Background

Aim

Methods

Results

Conclusions

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Background

Aim

Methods

Results

Conclusions

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