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Journal of Ovarian Research

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Open Access 01-12-2014 | Research

microRNA 490-3P enhances the drug-resistance of human ovarian cancer cells

Authors: Shuo Chen, Xi Chen, Yin-Ling Xiu, Kai-Xuan Sun, Zhi-Hong Zong, Yang Zhao

Published in: Journal of Ovarian Research | Issue 1/2014

Abstract

Background

MicroRNAs (miRNAs) are non-coding, single-stranded small RNAs that regulate gene expression negatively, which is involved in fundamental cellular processes. In this study, we investigated the role of miR-490-3P in the development of drug resistance in ovarian cancer cells.

Methods

The human ovarian carcinoma cell line A2780 and A2780/Taxol were exposed to paclitaxel in the presence or absence of microRNA 490-3P transfection, after which cell viability were performed by CCK-8 assay. Reverse transcription polymerase chain reaction (RT-PCR) and western blotting were used to assess the mRNA and protein expression levels of GST-π, MDR1 or P-gp.

Results

Our results showed higher miR-490-3P mRNA expression level in A2780/Taxol cells than in A2780 cells (p < 0.05). Following miR-490-3P transfection, both A2780 and A2780/Taxol cells showed decreased sensitivity to paclitaxel. The mRNA expression levels of MDR1, GST-π (p < 0.05) and protein expression levels of P-gp, GST-π were down-regulated after miR-490-3P transfection in comparison to mock and negative control cancer cells.

Conclusion

Our results demonstrate for the first time that microRNA 490-3P may be involved in the development of drug resistance in ovarian cancer.

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Title: microRNA 490-3P enhances the drug-resistance of human ovarian cancer cells
Authors: Shuo Chen
Xi Chen
Yin-Ling Xiu
Kai-Xuan Sun
Zhi-Hong Zong
Yang Zhao
Publication date: 01-12-2014
Publisher: BioMed Central
Published in: Journal of Ovarian Research / Issue 1/2014
Electronic ISSN: 1757-2215
DOI: https://doi.org/10.1186/s13048-014-0084-4

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

microRNA 490-3P enhances the drug-resistance of human ovarian cancer cells

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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