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Journal of Hematology & Oncology
Published in: Journal of Hematology & Oncology 1/2016

Open Access 01-12-2016 | Research highlight

Chasing the precursor of functional hematopoietic stem cells at the single cell levels in mouse embryos

Authors: Xiaochen Wang, Yuemin Gong, Hideo Ema

Published in: Journal of Hematology & Oncology | Issue 1/2016

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Abstract

Background

Adult hematopoietic stem cells (HSCs), the ideal system for regenerative research, were isolated at single cell levels decades ago, whereas studies on embryonic HSCs are much more difficult.

Methods

Zhou et al identified a new pre-HSC cell surface marker, CD201, by which they isolated pre-HSCs at single cell levels for further analyses.

Results

The novel expression pattern of HSC development is revealed, including the fundamental role of mammalian targets of rapamycin (mTOR) signaling pathway in HSCs emergence, and the repopulation potential of S/G2/M phase pre-HSCs.

Conclusions

Deeper understandings of the cellular origin and developmental regulatory network of HSCs are essential to develop new strategies of generating HSCs in vitro for clinical application.
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Liu X, et al. DNA methyltransferase 1 functions through C/ebpa to maintain hematopoietic stem and progenitor cells in zebrafish. J Hematol Oncol. 2015;8:15.CrossRefPubMedPubMedCentral
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Osawa M, et al. Long-term lymphohematopoietic reconstitution by a single CD34-low/negative hematopoietic stem cell. Science. 1996;273(5272):242–5.CrossRefPubMed
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Zhou F, et al. Tracing haematopoietic stem cell formation at single-cell resolution. Nature. 2016;533(7604):487–92.CrossRefPubMed
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Metadata
Title
Chasing the precursor of functional hematopoietic stem cells at the single cell levels in mouse embryos
Authors
Xiaochen Wang
Yuemin Gong
Hideo Ema
Publication date
01-12-2016
Publisher
BioMed Central
Published in
Journal of Hematology & Oncology / Issue 1/2016
Electronic ISSN: 1756-8722
DOI
https://doi.org/10.1186/s13045-016-0289-7

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