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Open Access 01-12-2016 | Research

Anti-proliferative effects of T cells expressing a ligand-based chimeric antigen receptor against CD116 on CD34⁺ cells of juvenile myelomonocytic leukemia

Authors: Yozo Nakazawa, Kazuyuki Matsuda, Takashi Kurata, Akane Sueki, Miyuki Tanaka, Kazuo Sakashita, Chihaya Imai, Matthew H. Wilson, Kenichi Koike

Published in: Journal of Hematology & Oncology | Issue 1/2016

Abstract

Background

Juvenile myelomonocytic leukemia (JMML) is a fatal, myelodysplastic/myeloproliferative neoplasm of early childhood. Patients with JMML have mutually exclusive genetic abnormalities in granulocyte-macrophage colony-stimulating factor (GM-CSF) receptor (GMR, CD116) signaling pathway. Allogeneic hematopoietic stem cell transplantation is currently the only curative treatment option for JMML; however, disease recurrence is a major cause of treatment failure. We investigated adoptive immunotherapy using GMR-targeted chimeric antigen receptor (CAR) for JMML.

Methods

We constructed a novel CAR capable of binding to GMR via its ligand, GM-CSF, and generated piggyBac transposon-based GMR CAR-modified T cells from three healthy donors and two patients with JMML. We further evaluated the anti-proliferative potential of GMR CAR T cells on leukemic CD34⁺ cells from six patients with JMML (two NRAS mutations, three PTPN11 mutations, and one monosomy 7), and normal CD34⁺ cells.

Results

GMR CAR T cells from healthy donors suppressed the cytokine-dependent growth of MO7e cells, but not the growth of K562 and Daudi cells. Co-culture of healthy GMR CAR T cells with CD34⁺ cells of five patients with JMML at effector to target ratios of 1:1 and 1:4 for 2 days significantly decreased total colony growth, regardless of genetic abnormality. Furthermore, GMR CAR T cells from a non-transplanted patient and a transplanted patient inhibited the proliferation of respective JMML CD34⁺ cells at onset to a degree comparable to healthy GMR CAR T cells. Seven-day co-culture of GMR CAR T cells resulted in a marked suppression of JMML CD34⁺ cell proliferation, particularly CD34⁺CD38⁻ cell proliferation stimulated with stem cell factor and thrombopoietin on AGM-S3 cells. Meanwhile, GMR CAR T cells exerted no effects on normal CD34⁺ cell colony growth.

Conclusions

Ligand-based GMR CAR T cells may have anti-proliferative effects on stem and progenitor cells in JMML.

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Title: Anti-proliferative effects of T cells expressing a ligand-based chimeric antigen receptor against CD116 on CD34+ cells of juvenile myelomonocytic leukemia
Authors: Yozo Nakazawa
Kazuyuki Matsuda
Takashi Kurata
Akane Sueki
Miyuki Tanaka
Kazuo Sakashita
Chihaya Imai
Matthew H. Wilson
Kenichi Koike
Publication date: 01-12-2016
Publisher: BioMed Central
Published in: Journal of Hematology & Oncology / Issue 1/2016
Electronic ISSN: 1756-8722
DOI: https://doi.org/10.1186/s13045-016-0256-3

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Abstract

Background

Methods

Results

Conclusions

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