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Journal of Hematology & Oncology
Published in: Journal of Hematology & Oncology 1/2016

Open Access 01-12-2016 | Research

An engineered multicomponent bone marrow niche for the recapitulation of hematopoiesis at ectopic transplantation sites

Authors: Mónica S. Ventura Ferreira, Christian Bergmann, Isabelle Bodensiek, Kristina Peukert, Jessica Abert, Rafael Kramann, Paul Kachel, Björn Rath, Stephan Rütten, Ruth Knuchel, Benjamin L. Ebert, Horst Fischer, Tim H. Brümmendorf, Rebekka K. Schneider

Published in: Journal of Hematology & Oncology | Issue 1/2016

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Abstract

Background

Bone marrow (BM) niches are often inaccessible for controlled experimentation due to their difficult accessibility, biological complexity, and three-dimensional (3D) geometry.

Methods

Here, we report the development and characterization of a BM model comprising of cellular and structural components with increased potential for hematopoietic recapitulation at ectopic transplantation sites. Cellular components included mesenchymal stromal cells (MSCs) and hematopoietic stem and progenitor cells (HSPCs). Structural components included 3D β-tricalcium phosphate (β-TCP) scaffolds complemented with Matrigel or collagen I/III gels for the recreation of the osteogenic/extracellular character of native BM.

Results

In vitro, β-TCP/Matrigel combinations robustly maintained proliferation, osteogenic differentiation, and matrix remodeling capacities of MSCs and maintenance of HSPCs function over time. In vivo, scaffolds promoted strong and robust recruitment of hematopoietic cells to sites of ectopic transplantation, vascularization, and soft tissue formation.

Conclusions

Our tissue-engineered BM system is a powerful tool to explore the regulatory mechanisms of hematopoietic stem and progenitor cells for a better understanding of hematopoiesis in health and disease.
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Metadata
Title
An engineered multicomponent bone marrow niche for the recapitulation of hematopoiesis at ectopic transplantation sites
Authors
Mónica S. Ventura Ferreira
Christian Bergmann
Isabelle Bodensiek
Kristina Peukert
Jessica Abert
Rafael Kramann
Paul Kachel
Björn Rath
Stephan Rütten
Ruth Knuchel
Benjamin L. Ebert
Horst Fischer
Tim H. Brümmendorf
Rebekka K. Schneider
Publication date
01-12-2016
Publisher
BioMed Central
Published in
Journal of Hematology & Oncology / Issue 1/2016
Electronic ISSN: 1756-8722
DOI
https://doi.org/10.1186/s13045-016-0234-9

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