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Chinese Medicine
Published in: Chinese Medicine 1/2017

Open Access 01-12-2017 | Research

Metabolic regulations of a decoction of Hedyotis diffusa in acute liver injury of mouse models

Authors: Min Dai, Fenglin Wang, Zengcheng Zou, Gemin Xiao, Hongjie Chen, Hongzhi Yang

Published in: Chinese Medicine | Issue 1/2017

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Abstract

Background

Dysfunctional metabolisms are contributed to LPS/GALN-induced hepatitis. However, whether Hedyotis diffusa (HD) employs metabolic strategies against hepatitis is unknown.

Methods

We use the cytokines expression, levels of serum alanine transaminase and aspartate transaminase, survival and histological analysis to measure the effect of decoction of HD on acute severe hepatitis of mouse induced by LPS/GALN. Meanwhile, we utilize GC/MS-based metabolomics to characterize the variation of metabolomes.

Results

The present study shows the relieving liver damage in HD decoction-treated mice. Metabolic category using differential metabolites indicates the lower percentage of carbohydrates in LPS/GALN + HD group than LPS/GALN group, revealing the value of carbohydrate metabolism in HD decoction-administrated mouse liver. Further pathway enrichment analysis proposes that citrate cycle, galactose metabolism, and starch and sucrose metabolism are three important carbohydrate metabolisms that involve in the protective effect of decoction of HD during acute hepatitis. Furthermore, other important enrichment pathways are biosynthesis of unsaturated fatty acids, alanine, aspartate and glutamate metabolism, and arginine and proline metabolism. Fatty acids or amino acids involved in above-mentioned pathways are also detected in high loading distribution on IC01 and IC02, thereby manifesting the significance of these metabolites. Other key metabolites detect in ICA analysis were cholesterol, lactic acid and tryptophan.

Conclusions

The variation tendency of above-mentioned metabolites is totally consistent with the protective nature of decoction of HD. These findings give a viewpoint that HD decoction-effected metabolic strategies are linked to underlying mechanisms of decoction of HD and highlight the importance of metabolic mechanisms against hepatitis.
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Literature
1.
Fitzmorris P, Shoreibah M, Anand B, Singal A. Management of hepatocellular carcinoma. J Cancer Res Clin Oncol. 2015;141:861.CrossRefPubMed
2.
Luedde T, Kaplowitz N, Schwabe RF. Cell death and cell death responses in liver disease: mechanisms and clinical relevance. Gastroenterology. 2014;147(765–83):e4.
3.
Han MS, Barrett T, Brehm MA, Davis RJ. Inflammation mediated by JNK in myeloid cells promotes the development of hepatitis and hepatocellular carcinoma. Cell Rep. 2016;15:19–26.CrossRefPubMedPubMedCentral
4.
5.
Ganbold M, Barker J, Ma R, Jones L, Carew M. Cytotoxicity and bioavailability studies on a decoction of Oldenlandia diffusa and its fractions separated by HPLC. J Ethnopharmacol. 2010;131:396–403.CrossRefPubMed
6.
Ye JH, Liu MH, Zhang XL, He JY. Chemical profiles and protective effect of Hedyotis diffusa Willd in lipopolysaccharide-induced renal inflammation mice. Int J Mol Sci. 2015;16:27252–69.CrossRefPubMedPubMedCentral
7.
Niu Y, Meng QX. Chemical and preclinical studies on Hedyotis diffusa with anticancer potential. J Asian Nat Prod Res. 2013;15:550–65.CrossRefPubMed
8.
Ahmad R, Shaari K, Lajis NH, Hamzah AS, Ismail NH, Kitajima M. Anthraquinones from Hedyotis capitellata. Phytochemistry. 2005;66:1141–7.CrossRefPubMed
9.
Li C, Xue X, Zhou D, Zhang F, Xu Q, Ren L, et al. Analysis of iridoid glucosides in Hedyotis diffusa by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry. J Pharm Biomed Anal. 2008;48:205–11.CrossRefPubMed
10.
Lin J, Chen Y, Wei L, Chen X, Xu W, Hong Z, et al. Hedyotis diffusa Willd extract induces apoptosis via activation of the mitochondrion-dependent pathway in human colon carcinoma cells. Int J Oncol. 2010;37:1331–8.CrossRefPubMed
11.
Lin J, Wei L, Xu W, Hong Z, Liu X, Peng J. Effect of Hedyotis diffusa Willd extract on tumor angiogenesis. Mol Med Rep. 2011;4:1283–8.CrossRefPubMed
12.
Lin CC, Kuo CL, Lee MH, Hsu SC, Huang AC, Tang NY, et al. Extract of Hedyotis diffusa Willd influences murine leukemia WEHI-3 cells in vivo as well as promoting T-and B-cell proliferation in leukemic mice. In Vivo. 2011;25:633–40.PubMed
13.
Kuo YJ, Lin JP, Hsiao YT, Chou GL, Tsai YH, Chiang SY, et al. Ethanol extract of Hedyotis diffusa Willd affects immune responses in normal Balb/c mice in vivo. In Vivo. 2015;29:453–60.PubMed
14.
15.
16.
Cairns RA, Harris IS, Mak TW. Regulation of cancer cell metabolism. Nat Rev Cancer. 2011;11:85–95.CrossRefPubMed
17.
18.
Wang Z, Gao K, Xu C, Gao J, Yan Y, Wang Y, et al. Metabolic effects of Hedyotis diffusa on rats bearing Walker 256 tumor revealed by NMR-based metabolomics. Magn Reson Chem. 2017;56:5–17.CrossRefPubMed
19.
Sun H, Zhang A, Yan G, Piao C, Li W, Sun C, et al. Metabolomic analysis of key regulatory metabolites in hepatitis C virus–infected tree shrews. Mol Cell Proteom. 2013;12:710–9.CrossRef
20.
Kuehnbaum NL, Britz-McKibbin P. New advances in separation science for metabolomics: resolving chemical diversity in a post-genomic era. Chem Rev. 2013;113:2437–68.CrossRefPubMed
21.
Besada C, Sanchez G, Salvador A, Granell A. Volatile compounds associated to the loss of astringency in persimmon fruit revealed by untargeted GC–MS analysis. Metabolomics. 2013;9:157–72.CrossRef
22.
23.
Chen XH, Liu SR, Peng B, Li D, Cheng ZX, Zhu JX, et al. Exogenous l-valine promotes phagocytosis to kill multidrug-resistant bacterial pathogens. Front Immunol. 2017;8:207.PubMedPubMedCentral
24.
Ma L, Gong H, Zhu H, Ji Q, Su P, Liu P, et al. A novel small-molecule tumor necrosis factor α inhibitor attenuates inflammation in a hepatitis mouse model. J Biol Chem. 2014;289:12457–66.CrossRefPubMedPubMedCentral
25.
Chen XH, Zhang BW, Li H, Peng XX. Myo-inositol improves the host’s ability to eliminate balofloxacin-resistant Escherichia coli. Sci Rep. 2015;5:10720.CrossRefPubMedPubMedCentral
26.
Sreekumar A, Poisson LM, Rajendiran TM, Khan AP, Cao Q, Yu J, et al. Metabolomic profiles delineate potential role for sarcosine in prostate cancer progression. Nature. 2009;457:910–4.CrossRefPubMedPubMedCentral
27.
Amathieu R, Nahon P, Triba M, Bouchemal N, Trinchet JC, Beaugrand M, et al. Metabolomic approach by 1H NMR spectroscopy of serum for the assessment of chronic liver failure in patients with cirrhosis. J Proteome Res. 2011;10:3239–45.CrossRefPubMed
28.
Naccarato WF, Ray RE, Wells WW. Biosynthesis of myo-inositol in rat mammary gland. Isolation and properties of the enzymes. Arch Biochem Biophys. 1974;164:194–201.CrossRefPubMed
29.
Wienkoop S, Morgenthal K, Wolschin F, Scholz M, Selbig J, Weckwerth W. Integration of metabolomic and proteomic phenotypes analysis of data covariance dissects starch and RFO metabolism from low and high temperature compensation response in Arabidopsis thaliana. Mol Cell Proteom. 2008;7:1725–36.CrossRef
30.
Kim SJ, Chung WS, Kim SS, Ko SG, Um JY. Antiinflammatory effect of Oldenlandia diffusa and its constituent, hentriacontane, through suppression of caspase-1 activation in mouse peritoneal macrophages. Phytother Res. 2011;25:1537–46.CrossRefPubMed
31.
Chou TW, Feng JH, Huang CC, Cheng YW, Chien SC, Wang SY, et al. A plant kavalactone desmethoxyyangonin prevents inflammation and fulminant hepatitis in mice. PLoS ONE. 2013;8:e77626.CrossRefPubMedPubMedCentral
32.
Tannahill GM, Curtis AM, Adamik J, Palsson-McDermott EM, McGettrick AF, Goel G, et al. Succinate is an inflammatory signal that induces IL-1β through HIF-1α. Nature. 2013;496:238–42.CrossRefPubMedPubMedCentral
33.
Krawczyk CM, Holowka T, Sun J, Blagih J, Amiel E, DeBerardinis RJ, et al. Toll-like receptor–induced changes in glycolytic metabolism regulate dendritic cell activation. Blood. 2010;115:4742–9.CrossRefPubMedPubMedCentral
34.
Cheng SC, Joosten LA, Netea MG. The interplay between central metabolism and innate immune responses. Cytokine Growth Factor Rev. 2014;25:707–13.CrossRefPubMed
35.
Isaacs JS, Jung YJ, Mole DR, Lee S, Torres-Cabala C, Chung YL, et al. HIF overexpression correlates with biallelic loss of fumarate hydratase in renal cancer: novel role of fumarate in regulation of HIF stability. Cancer Cell. 2005;8:143–53.CrossRefPubMed
36.
Guo CH, Sun TT, Weng XD, Zhang JC, Chen JX, Deng GJ. The investigation of glucose metabolism and insulin secretion in subjects of chronic hepatitis B with cirrhosis. Int J Clin Exp Pathol. 2015;8:13381.PubMedPubMedCentral
37.
Jiang WD, Hu K, Liu Y, Jiang J, Wu P, Zhao J, et al. Dietary myo-inositol modulates immunity through antioxidant activity and the Nrf2 and E2F4/cyclin signalling factors in the head kidney and spleen following infection of juvenile fish with Aeromonas hydrophila. Fish Shellfish Immunol. 2016;49:374–86.CrossRefPubMed
38.
Shi H, Kokoeva MV, Inouye K, Tzameli I, Yin H, Flier JS. TLR4 links innate immunity and fatty acid—induced insulin resistance. J Clin Invest. 2006;116:3015–25.CrossRefPubMedPubMedCentral
39.
Eguchi K, Manabe I, Oishi-Tanaka Y, Ohsugi M, Kono N, Ogata F, et al. Saturated fatty acid and TLR signaling link β cell dysfunction and islet inflammation. Cell Metab. 2012;15:518–33.CrossRefPubMed
40.
41.
42.
Singleton KD, Beckey VE, Wischmeyer PE. Glutamine prevents activation of NF-κB and stress kinase pathways, attenuates inflammatory cytokine release, and prevents acute respiratory distress syndrome (ARDS) following sepsis. Shock. 2005;24:583–9.CrossRefPubMed
43.
Hou YC, Liu JJ, Pai MH, Tsou SS, Yeh SL. Alanyl-glutamine administration suppresses Th17 and reduces inflammatory reaction in dextran sulfate sodium-induced acute colitis. Int Immunopharmacol. 2013;17:1–8.CrossRefPubMed
44.
Hou YC, Wu JM, Wang MY, Wu MH, Chen KY, Yeh SL, et al. Glutamine supplementation attenuates expressions of adhesion molecules and chemokine receptors on T cells in a murine model of acute colitis. Mediators Inflamm. 2014;2014:837107.CrossRefPubMedPubMedCentral
45.
46.
47.
Wheeler MD, Thurman RG. Production of superoxide and TNF-α from alveolar macrophages is blunted by glycine. Am J Physiol. 1999;277:L952–9.PubMed
48.
Konashi S, Takahashi K, Akiba Y. Effects of dietary essential amino acid deficiencies on immunological variables in broiler chickens. Br J Nutr. 2000;83:449–56.PubMed
49.
Morris SM Jr. Arginine: master and commander in innate immune responses. Sci Signal. 2010;3:e27.CrossRef
50.
Platten M, Ho PP, Youssef S, Fontoura P, Garren H, Hur EM, et al. Treatment of autoimmune neuroinflammation with a synthetic tryptophan metabolite. Science. 2005;310:850–5.CrossRefPubMed
51.
Stone TW, Stoy N, Darlington LG. An expanding range of targets for kynurenine metabolites of tryptophan. Trends Pharmacol Sci. 2013;34:136–43.CrossRefPubMed
Metadata
Title
Metabolic regulations of a decoction of Hedyotis diffusa in acute liver injury of mouse models
Authors
Min Dai
Fenglin Wang
Zengcheng Zou
Gemin Xiao
Hongjie Chen
Hongzhi Yang
Publication date
01-12-2017
Publisher
BioMed Central
Published in
Chinese Medicine / Issue 1/2017
Electronic ISSN: 1749-8546
DOI
https://doi.org/10.1186/s13020-017-0159-4

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