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Open Access 01-12-2017 | Research article

Enumeration of circulating endothelial cell frequency as a diagnostic marker in aortic valve surgery - a flow cytometric approach

Authors: Anton Sabashnikov, Klaus Neef, Vera Chesnokova, Leonie Wegener, Kathrin Godthardt, Maximilian Scherner, Elmar W. Kuhn, Antje-Christin Deppe, Meike Lauer, Kaveh Eghbalzadeh, Mohamed Zeriouh, Parwis B. Rahmanian, Jens Wippermann, Ferdinand Kuhn-Régnier, Navid Madershahian, Thorsten Wahlers, Alexander Weymann, Yeong-Hoon Choi

Published in: Journal of Cardiothoracic Surgery | Issue 1/2017

Abstract

Background

The frequency of circulating endothelial cells (CEC) in patients’ peripheral blood can be assessed as a direct marker of endothelial damage. However, conventional enumeration methods are extremely challenging. We developed a novel, automated approach to determine CEC frequencies and tested this method on two groups of patients undergoing conventional (CAVR) versus trans-catheter aortic valve implantation (TAVI).

Methods

CEC frequencies were assessed by a flow cytometric approach, including automated pre-enrichment of CD34 positive blood cell subpopulation and isotype controls. The efficacy and reproducibility of the CEC enumeration method was validated by spiking blood samples of healthy control donors with defined numbers of endothelial cells.

Results

CEC frequencies were significantly higher in the TAVI group before (9.8 ± 4.1 vs. 5.5 ± 2.2, p = 0.019) and 1 h after surgery (13.4 ± 5.1 vs. 8.2 ± 4.1, p = 0.030) corresponding to higher Euroscore, STS score in higher risk patients from the TAVI group. Five days after surgery, CEC frequencies became significantly higher in the more invasive CAVR group (39.0 ± 13.0 vs. 14.3 ± 4.4, p < 0.001) compared to minimally invasive TAVI approach.

Conclusions

The new flow cytometric approach might be a robust and reliable method for CEC enumeration. Initial results show that CEC frequency is a valid clinical marker for the assessment of pre-operative risk, invasiveness of surgical procedure and clinical outcome. Further studies are necessary to validate the practical clinical usefulness and the potential superiority compared to conventional markers.

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Title: Enumeration of circulating endothelial cell frequency as a diagnostic marker in aortic valve surgery - a flow cytometric approach
Authors: Anton Sabashnikov
Klaus Neef
Vera Chesnokova
Leonie Wegener
Kathrin Godthardt
Maximilian Scherner
Elmar W. Kuhn
Antje-Christin Deppe
Meike Lauer
Kaveh Eghbalzadeh
Mohamed Zeriouh
Parwis B. Rahmanian
Jens Wippermann
Ferdinand Kuhn-Régnier
Navid Madershahian
Thorsten Wahlers
Alexander Weymann
Yeong-Hoon Choi
Publication date: 01-12-2017
Publisher: BioMed Central
Published in: Journal of Cardiothoracic Surgery / Issue 1/2017
Electronic ISSN: 1749-8090
DOI: https://doi.org/10.1186/s13019-017-0631-3

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Enumeration of circulating endothelial cell frequency as a diagnostic marker in aortic valve surgery - a flow cytometric approach

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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