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Journal of Orthopaedic Surgery and Research

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Open Access 01-12-2016 | Research article

The expression of SIRT1 in articular cartilage of patients with knee osteoarthritis and its correlation with disease severity

Authors: Yusheng Li, Wenfeng Xiao, Ping Wu, Zhenhan Deng, Chao Zeng, Hui Li, Tuo Yang, Guanghua Lei

Published in: Journal of Orthopaedic Surgery and Research | Issue 1/2016

Abstract

Background

The study aims to investigate the expression of SIRT1 in articular cartilage of patients with primary knee osteoarthritis (OA) and its relationship with disease severity.

Methods

Cartilage tissue samples were collected from 38 knee OA patients and 9 normal healthy controls and then ascribed to normal, mild, moderate, and severe groups on the basis of the improved Mankin grading system. The expression of SIRT1 in articular cartilage was detected by immunohistochemistry and western blots. The expression of p53 and acetylated p53 (Ac-p53) was also measured by western blots.

Results

The mutual comparisons of the SIRT1 expression levels in all groups have statistical significance except the one between the mild and moderate groups. Moreover, western blot results showed that the SIRT1 was decreased and p53/Ac-p53 were increased in the OA group. The average gray level of SIRT1 increases with the improving grade of the improved Mankin grading system scorers.

Conclusions

The expression of SIRT1 in articular cartilage is negatively associated with severity of knee OA, indicating that SIRT1 may act as a monitoring indicator for determining development and progression of knee OA.

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Title: The expression of SIRT1 in articular cartilage of patients with knee osteoarthritis and its correlation with disease severity
Authors: Yusheng Li
Wenfeng Xiao
Ping Wu
Zhenhan Deng
Chao Zeng
Hui Li
Tuo Yang
Guanghua Lei
Publication date: 01-12-2016
Publisher: BioMed Central
Published in: Journal of Orthopaedic Surgery and Research / Issue 1/2016
Electronic ISSN: 1749-799X
DOI: https://doi.org/10.1186/s13018-016-0477-8

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

The expression of SIRT1 in articular cartilage of patients with knee osteoarthritis and its correlation with disease severity

Abstract

Background

Methods

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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