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Open Access 01-12-2018 | Methodology

Clinical comparison of liquid-based and conventional cytology of oral brush biopsies: a randomized controlled trial

Authors: Constanze Olms, Nathalie Hix, Heinrich Neumann, Maryam Yahiaoui-Doktor, Torsten W. Remmerbach

Published in: Head & Face Medicine | Issue 1/2018

Abstract

Background

Exfoliative cytology performed on oral brush samples can help dentists to decide, whether a given oral lesion is (pre-) malignant. The use of non-invasive brush biopsies as an auxiliary tool in the diagnosis of oral mucosal lesions has gained renewed interest since improvements in cytological techniques such as the development of adjuvant diagnostic tools and liquid-based cell preparation techniques.

Methods

The aim of this study was to compare the quality of two different preparation techniques (cell collectors): the conventional transfer procedure to glass slides and the so-called liquid-based cytology preparation method. Cell smears were collected from 10 orally healthy individuals (mean age: 24 years) from the palatine mucosa at two different times (baseline and 4 weeks later). Slides of both techniques were stained by Giemsa (n = 40) and May-Gruenwald Giemsa (n = 40). The statistical analysis was performed with Excel.

Results

On specimen analysis, the liquid-based cytology showed statistically significant improvement compared to conventional glass sides (p < 0.001). Thin layers, which were performed by liquid-based cytology showed significantly better results in the parameters (p < 0.001): uniform distribution, cellular overlapping, cellular disformation, mucus, microbial colonies and debris. The conventional glass slides approach showed more cell overlapping and contamination with extraneous material than thin layers, which were performed by Orcellex® Brush cell collectors.

Conclusions

Both techniques are diagnostically reliable. The liquid-based method showed an overall improvement on sample preservation, specimen adequacy, visualization of cell morphology and reproducibility.

Liquid-based cytology simplifies cell collection due to easier handling and less transfer errors by dentists.

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Title: Clinical comparison of liquid-based and conventional cytology of oral brush biopsies: a randomized controlled trial
Authors: Constanze Olms
Nathalie Hix
Heinrich Neumann
Maryam Yahiaoui-Doktor
Torsten W. Remmerbach
Publication date: 01-12-2018
Publisher: BioMed Central
Published in: Head & Face Medicine / Issue 1/2018
Electronic ISSN: 1746-160X
DOI: https://doi.org/10.1186/s13005-018-0166-4

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Clinical comparison of liquid-based and conventional cytology of oral brush biopsies: a randomized controlled trial

Abstract

Background

Methods

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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