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Open Access 01-12-2015 | Short report

MiR-21, miR-34a, miR-198 and miR-217 as diagnostic and prognostic biomarkers for chronic pancreatitis and pancreatic ductal adenocarcinoma

Authors: Petra Vychytilova-Faltejskova, Igor Kiss, Sona Klusova, Jan Hlavsa, Vladimir Prochazka, Zdenek Kala, Jan Mazanec, Jitka Hausnerova, Leos Kren, Marketa Hermanova, Jiri Lenz, Petr Karasek, Rostislav Vyzula, Ondrej Slaby

Published in: Diagnostic Pathology | Issue 1/2015

Abstract

Background

Pancreatic ductal adenocarcinoma is an aggressive malignancy with late presentation, metastatic potential and very poor prognosis. Therefore, there is an urgent need for novel diagnostic and prognostic biomarkers. MicroRNAs are small non-coding RNAs that post-transcriptionally regulate gene expression. Altered expression of microRNAs has been reported in wide range of malignancies, including pancreatic ductal adenocarcinoma. The aim of this study was to analyze the expression of selected microRNAs in normal pancreas, chronic pancreatitis and pancreatic ductal adenocarcinoma tissues and evaluate their diagnostic and prognostic potential.

Findings

Using quantitative real-time PCR, expression levels of 4 microRNAs were examined in 74 tumor tissues, 18 tissues of chronic pancreatitis and 9 adjacent normal tissues and correlated with clinicopathological features of patients. Expression levels of miR-21, miR-34a and miR-198 were significantly higher, whereas levels of miR-217 were significantly lower in pancreatic ductal adenocarcinomas compared to healthy tissues and tissues of chronic pancreatitis. Moreover, increased expression of miR-21 and miR-198 was significantly associated with shorter disease free survival and overall survival.

Conclusions

Our data suggest that altered expression of examined microRNAs is related to neoplastic transformation and progression of the disease and these microRNAs could serve as diagnostic and prognostic biomarkers for pancreatic ductal adenocarcinoma.

Virtual slides

The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1373952531543898

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Title: MiR-21, miR-34a, miR-198 and miR-217 as diagnostic and prognostic biomarkers for chronic pancreatitis and pancreatic ductal adenocarcinoma
Authors: Petra Vychytilova-Faltejskova
Igor Kiss
Sona Klusova
Jan Hlavsa
Vladimir Prochazka
Zdenek Kala
Jan Mazanec
Jitka Hausnerova
Leos Kren
Marketa Hermanova
Jiri Lenz
Petr Karasek
Rostislav Vyzula
Ondrej Slaby
Publication date: 01-12-2015
Publisher: BioMed Central
Published in: Diagnostic Pathology / Issue 1/2015
Electronic ISSN: 1746-1596
DOI: https://doi.org/10.1186/s13000-015-0272-6

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

MiR-21, miR-34a, miR-198 and miR-217 as diagnostic and prognostic biomarkers for chronic pancreatitis and pancreatic ductal adenocarcinoma

Abstract

Background

Findings

Conclusions

Virtual slides

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Background

Findings

Conclusions

Virtual slides

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