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Open Access 01-12-2023 | Rabeprazole | Research

Identification of small molecules capable of enhancing viral membrane fusion

Authors: Mª Jesús García-Murria, Laura Gadea-Salom, Sandra Moreno, Marina Rius-Salvador, Oscar Zaragoza, Alejandro Brun, Ismael Mingarro, Luis Martínez-Gil

Published in: Virology Journal | Issue 1/2023

Abstract

Several approaches have been developed to analyze the entry of highly pathogenic viruses. In this study, we report the implementation of a Bimolecular Multicellular Complementation (BiMuC) assay to safely and efficiently monitor SARS-CoV-2 S-mediated membrane fusion without the need for microscopy-based equipment. Using BiMuC, we screened a library of approved drugs and identified compounds that enhance S protein-mediated cell-cell membrane fusion. Among them, ethynylestradiol promotes the growth of SARS-CoV-2 and Influenza A virus in vitro. Our findings demonstrate the potential of BiMuC for identifying small molecules that modulate the life cycle of enveloped viruses, including SARS-CoV-2.

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Title: Identification of small molecules capable of enhancing viral membrane fusion
Authors: Mª Jesús García-Murria
Laura Gadea-Salom
Sandra Moreno
Marina Rius-Salvador
Oscar Zaragoza
Alejandro Brun
Ismael Mingarro
Luis Martínez-Gil
Publication date: 01-12-2023
Publisher: BioMed Central
Keywords: Rabeprazole
SARS-CoV-2
Influenza
Published in: Virology Journal / Issue 1/2023
Electronic ISSN: 1743-422X
DOI: https://doi.org/10.1186/s12985-023-02068-1

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