Published in:
Open Access
01-12-2022 | Arthropods | Research
Negeviruses isolated from mosquitoes in the Brazilian Amazon
Authors:
Ana Cláudia da Silva Ribeiro, Lívia Caricio Martins, Sandro Patroca da Silva, Daniele Barbosa de Almeida Medeiros, Keissy Karoline Pinheiro Miranda, Joaquim Pinto Nunes Neto, Hamilton Antônio de Oliveira Monteiro, Bruna Lais Sena do Nascimento, Jose Wilson Rosa Junior, Ana Cecilia Ribeiro Cruz, Pedro Fernando da Costa Vasconcelos, Valéria Lima Carvalho, Sueli Guerreiro Rodrigues
Published in:
Virology Journal
|
Issue 1/2022
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Abstract
Background
There are several groups of viruses including Insect Specific Viruses (ISV) such as the taxon Negevirus, a group of viruses phylogenetically related to plant viruses. Negeviruses replicate in mosquito cells, but not in vertebrate cells.
Methods
Pools of hematophagous arthropods were inoculated in Vero and C6/36 cells. The cells were observed to detect possible cytopathic effect. Then, indirect immunofluorescence, RT-PCR, and nucleotide sequencing were performed.
Results
Seven samples which presented negative results for flaviviruses, alphaviruses and bunyaviruses, but showed cytopathic effect in C6/36 cells were sequenced. We identified the occurrence of a variety of ISVs, most of them belonging to the taxon Negevirus: The Brejeira, Negev, Cordoba and Wallerfield viruses, including a new virus for science, tentatively named Feitosa virus.
Conclusions
We detected negeviruses in the Amazon region, including two viruses that were isolated for the first time in Brazil: Cordoba virus and the Negev virus and, a new virus for science: the Feitosa virus.