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Virology Journal

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Open Access 01-12-2020 | Influenza Virus | Research

CD83 expression regulates antibody production in response to influenza A virus infection

Authors: Madhav Akauliya, Avishekh Gautam, Sony Maharjan, Byoung Kwon Park, Jinsoo Kim, Hyung-Joo Kwon

Published in: Virology Journal | Issue 1/2020

Abstract

Background

CD83 is known to regulate lymphocyte maturation, activation, homeostasis, and antibody response to immunization and infection. While CD83 has a major part in B cell function, its role in influenza A virus infection has not yet been investigated.

Methods

We investigated the role of CD83 using C57BL/6J wild type mice and CD83 knockout (KO) mice after intraperitoneal administration of the influenza A/WSN/1933 virus. We analyzed cells of the peritoneal cavity, splenocytes, and cells of the bone marrow with FACS to investigate CD83 expression and cell population change in response to the virus infection. ELISA was performed with sera and peritoneal cavity fluids to detect A/WSN/1933 virus-specific IgG and the subclasses of IgG.

Results

FACS analysis data showed a transient but distinct induction of CD83 expression in the peritoneal B cells of wild type mice. CD83 KO mice exhibited a delayed recovery of B cells in the bone marrow after influenza virus infection and overall, a smaller T cell population compared to wild type mice. The peritoneal cavity and serum of the wild type mice contained a high titer of IgG within 14 days after infection, whereas the CD83 KO mice had a very low titer of IgG.

Conclusions

These results show the importance of CD83 in lymphocytes homeostasis and antibody production during influenza A virus infection.

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Title: CD83 expression regulates antibody production in response to influenza A virus infection
Authors: Madhav Akauliya
Avishekh Gautam
Sony Maharjan
Byoung Kwon Park
Jinsoo Kim
Hyung-Joo Kwon
Publication date: 01-12-2020
Publisher: BioMed Central
Keywords: Influenza Virus
Influenza
Published in: Virology Journal / Issue 1/2020
Electronic ISSN: 1743-422X
DOI: https://doi.org/10.1186/s12985-020-01465-0

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CD83 expression regulates antibody production in response to influenza A virus infection

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Conclusions

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