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01-12-2020 | Human Cytomegalovirus | Research

Association of human cytomegalovirus (HCMV) neutralizing antibodies with antibodies to the HCMV glycoprotein complexes

Authors: Miho Shibamura, Tomoki Yoshikawa, Souichi Yamada, Takuya Inagaki, Phu Hoang Anh Nguyen, Hikaru Fujii, Shizuko Harada, Shuetsu Fukushi, Akira Oka, Masashi Mizuguchi, Masayuki Saijo

Published in: Virology Journal | Issue 1/2020

Abstract

Background

Human cytomegalovirus (HCMV) causes asymptomatic infections, but also causes congenital infections when women were infected with HCMV during pregnancy, and life-threatening diseases in immunocompromised patients. To better understand the mechanism of the neutralization activity against HCMV, the association of HCMV NT antibody titers was assessed with the antibody titers against each glycoprotein complex (gc) of HCMV.

Methods

Sera collected from 78 healthy adult volunteers were used. HCMV Merlin strain and HCMV clinical isolate strain 1612 were used in the NT assay with the plaque reduction assay, in which both the MRC-5 fibroblasts cells and the RPE-1 epithelial cells were used. Glycoprotein complex of gB, gH/gL complexes (gH/gL/gO and gH/gL/UL128–131A [PC]) and gM/gN were selected as target glycoproteins. 293FT cells expressed with gB, gM/gN, gH/gL/gO, or PC, were prepared and used for the measurement of the antibody titers against each gc in an indirect immunofluorescence assay (IIFA). The correlation between the IIFA titers to each gc and the HCMV-NT titers was evaluated.

Results

There were no significant correlations between gB-specific IIFA titers and the HCMV-NT titers in epithelial cells or between gM/gN complex-specific IIFA titers and the HCMV-NT titers. On the other hand, there was a statistically significant positive correlation between the IIFA titers to gH/gL complexes and HCMV-NT titers.

Conclusions

The data suggest that the gH/gL complexes might be the major target to induce NT activity against HCMV.

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Title: Association of human cytomegalovirus (HCMV) neutralizing antibodies with antibodies to the HCMV glycoprotein complexes
Authors: Miho Shibamura
Tomoki Yoshikawa
Souichi Yamada
Takuya Inagaki
Phu Hoang Anh Nguyen
Hikaru Fujii
Shizuko Harada
Shuetsu Fukushi
Akira Oka
Masashi Mizuguchi
Masayuki Saijo
Publication date: 01-12-2020
Publisher: BioMed Central
Keyword: Human Cytomegalovirus
Published in: Virology Journal / Issue 1/2020
Electronic ISSN: 1743-422X
DOI: https://doi.org/10.1186/s12985-020-01390-2

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