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Open Access 01-12-2018 | Research

Molecular characterization of human adenovirus associated with acute respiratory infections in Cameroon from 2011 to 2014

Authors: Sebastien Kenmoe, Marie-Astrid Vernet, Jerôme Le Goff, Véronique Beng Penlap, Astrid Vabret, Richard Njouom

Published in: Virology Journal | Issue 1/2018

Abstract

Background

Human adenoviruses (HAdVs) cause a wide range of diseases worldwide, including respiratory infections. Studies on HAdV molecular epidemiology are limited in Cameroon. The purpose of this study is to document the different types HAdV circulating in Cameroon in children with acute respiratory infections.

Methods

Nasopharyngeal swabs were collected from 811 children under 15 years from 2011 to 2014. The HAdV detection was assessed by semi-quantitative generic PCR r-gene®. The HAdV-positive samples were typed by amplification and sequencing of partial hexon gene and a real-time PCR. Demographic data were collected and analyzed. The infection and hospitalization risk factors were assessed thought the Chi-square test.

Results

A total of 137/220 HAdV-positive samples were amplified successfully. Six species of HAdV (Mastadenovirus A to F) were detected with B (108/220) and C (47/220) being the predominant strains. Hospitalization and age were significantly associated to HAdV-B and HAdV-C respectively. Phylogenetic analysis of HAdV-B3 virus (18) and B7 (5) shows a conserved and a significant temporal stability in relation to the reference sequence (99.1 to 100% of similarity).

Conclusion

This study reported HAdV species and types detected in children with acute respiratory infections in Cameroon between September 2011 and July 2014. These results support further evaluation of the spatio-temporal circulation pattern of HAdV species and types in Cameroon.

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Title: Molecular characterization of human adenovirus associated with acute respiratory infections in Cameroon from 2011 to 2014
Authors: Sebastien Kenmoe
Marie-Astrid Vernet
Jerôme Le Goff
Véronique Beng Penlap
Astrid Vabret
Richard Njouom
Publication date: 01-12-2018
Publisher: BioMed Central
Published in: Virology Journal / Issue 1/2018
Electronic ISSN: 1743-422X
DOI: https://doi.org/10.1186/s12985-018-1064-x

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Conclusion

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