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Virology Journal
Published in: Virology Journal 1/2017

Open Access 01-12-2017 | Research

ZIKV infection effects changes in gene splicing, isoform composition and lncRNA expression in human neural progenitor cells

Authors: Benxia Hu, Yongxia Huo, Liping Yang, Guijun Chen, Minhua Luo, Jinlong Yang, Jumin Zhou

Published in: Virology Journal | Issue 1/2017

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Abstract

Background

The Zika virus (ZIKV) is a mosquito-borne flavivirus that causes microcephaly and Guillain-Barré syndrome in infected individuals. To obtain insights into the mechanism of ZIKV infection and pathogenesis, we analyzed the transcriptome of ZIKV infected human neural progenitor cells (hNPCs) for changes in alternative splicing (AS), gene isoform (ISO) composition and long noncoding RNAs (lncRNAs) expression.

Methods

We analyzed differentially expressed lncRNAs, AS, ISO from RNA-seq data in ZIKV infected hNPCs.

Results

We obtained 149 differentially expressed lncRNAs, including potential viral targets to modulate cellular processes such as cell cycle, apoptosis and immune response. The infection induced 262 cases of AS occurring in 229 genes, which were enriched in cell death, RNA processing, transport, and neuron development. Among 691 differentially expressed ISOs, upregulated ISOs were enriched in signaling, regulation of transcription, and amino acid biosynthesis, while downregulated ISOs were mostly enriched in cell cycle. Importantly, these analyses revealed specific links between ZIKV induced changes in cellular pathways and the type of changes in the host transcriptome, suggesting important regulatory mechanisms.

Conclusions

Our analyses revealed candidate lncRNAs, AS events and ISOs which may function in ZIKV infection induced cell cycle disruption, apoptosis and attenuation of neurogenesis, and shed light on the roles of lncRNAs, AS and ISOs in virus-host interactions, and would facilitate future studies of ZIKV infection and pathogenesis.
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Metadata
Title
ZIKV infection effects changes in gene splicing, isoform composition and lncRNA expression in human neural progenitor cells
Authors
Benxia Hu
Yongxia Huo
Liping Yang
Guijun Chen
Minhua Luo
Jinlong Yang
Jumin Zhou
Publication date
01-12-2017
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2017
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/s12985-017-0882-6

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