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Virology Journal
Published in: Virology Journal 1/2017

Open Access 01-12-2017 | Research

The prevalence of dengue virus serotypes in asymptomatic blood donors reveals the emergence of serotype 4 in Saudi Arabia

Author: Ahmed Mohamed Ashshi

Published in: Virology Journal | Issue 1/2017

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Abstract

Background

Transmission of dengue virus (DENV) through blood transfusion has been documented and hence screening for DENV during blood donation has been recently recommended by the American Association of Blood Banks and Centres of Disease Control and Prevention. DENV is endemic in the Western province of the Kingdom of Saudi Arabia (KSA) and serotypes 1, 2 and 3, but not 4, have been detected. However, little is known regarding the rates of DENV during blood donation in the kingdom. The aim of this study was therefore to measure the prevalence of dengue virus and its serotypes in eligible Saudi blood donors in the endemic Western region of KSA.

Methods

This was a cross-sectional study and serum samples were collected from 910 eligible Saudi male blood donors. DENV IgM and IgG antibodies were measured serologically by ELISA while viral serotypes were detected by a single step IVD CE certified multiplex RT-PCR kit.

Results

The overall prevalence was 39 and 5.5% for IgG+ and IgM+, respectively. There were 12 (1.3%) with exclusively IgM+, 317 (34.8%) exclusively IgG+ and 38 (4.2%) with dual IgM+/IgG+ donors. The overall prevalence was 3.2% (n = 29) and 2.3% (n = 21) for primary and secondary infections. PCR was positive in 5.5% (n = 50) and, DENV-2 (n = 24; 48%) was the most frequent serotype and was significantly higher than DENV-1 (20%; P = 0.02) and DENV-3 (2%; P = 0.1 × 10−5) but not DENV-4 (30%; P = 0.2). There was no significant difference between both DENV-4 and DENV-1 (P = 0.4). The combination of the PCR and serology findings showed that 22 (2.4%) and 28 (3.1%) donors had primary and secondary viremic infections, respectively.

Conclusions

The detected rates of DENV by PCR suggest a potential high risk of viral transmission by blood transfusion. To the best of our knowledge, this study is the first to report the detection of DENV-4 serotype in Saudi Arabia. More studies are required to measure the precise prevalence of DENV serotypes and their potential transmission rate during blood donation in the kingdom.
Literature
1.
Bhat VG, Chavan P, Ojha S, Nair PK. Challenges in the Laboratory Diagnosis and Management of Dengue Infections. Open Microbiol J. 2015;9:33–7.PubMedPubMedCentral
2.
3.
Fakeeh M, Zaki AM. Virologic and serologic surveillance for dengue fever in Jeddah, Saudi Arabia, 1994–1999. Am J Trop Med Hyg. 2001;65:764–7.CrossRefPubMed
4.
Fakeeh M, Zaki AM. Dengue in Jeddah, Saudi Arabia, 1994–2002. Dengue Bull. 2003;27:13–8.
5.
Khan NA, Azhar EI, El-Fiky S, Madani HH, Abuljadial MA, Ashshi AM, Turkistani AM, Hamouh EA. Clinical profile and outcome of hospitalized patients during first outbreak of dengue in Makkah, Saudi Arabia. Acta Trop. 2008;105:39–44.CrossRefPubMed
6.
Alhaeli A, Bahkali S, Ali A, Househ MS, El-Metwally AA. The epidemiology of Dengue fever in Saudi Arabia: A systematic review. J Infect Public Health. 2016;9:117–24.CrossRefPubMed
7.
Al-Azraqi TA, El Mekki AA, Mahfouz AA. Seroprevalence of dengue virus infection in Aseer and Jizan regions, Southwestern Saudi Arabia. Trans R Soc Trop Med Hyg. 2013;107:368–71.CrossRefPubMed
8.
El-Badry A, Al-Ali K. Prevalence and seasonal distribution of dengue mosquito, Aedes aegypti (Diptera: Culicidae) in Al-Madinah Al-Munawwarah, Saudi Arabia. J Entomol. 2010;7:80–8.CrossRef
9.
Kheir SM, Alahmed AM, Al Kuriji MA, Al Zubyani SF. Distribution and seasonal activity of mosquitoes in al Madinah Al Munwwrah, Saudi Arabia. J Egypt Soc Parasitol. 2010;40:215–27.PubMed
10.
Zaki A, Perera D, Jahan SS, Cardosa MJ. Phylogeny of dengue viruses circulating in Jeddah, Saudi Arabia: 1994 to 2006. Trop Med Int Health. 2008;13:584–92.CrossRefPubMed
11.
Al-Saeed MS, El-Kafrawy SA, Farraj SA, Al-Subhi TL, Othman NA, Alsultan A, Ben Helaby HG, Alshawdari MM, Hassan AM, Charrel RN, Azhar EI, Hashem AM. Phylogenetic characterization of circulating Dengue and Alkhumra Hemorrhagic Fever viruses in western Saudi Arabia and lack of evidence of Zika virus in the region: A retrospective study, 2010–2015. J Med Virol. 2017. doi:10.​1002/​jmv.​24785.PubMed
12.
Aziz AT, Al-Shami SA, Mahyoub JA, Hatabbi M, Ahmad AH, Rawi CS. An update on the incidence of dengue gaining strength in Saudi Arabia and current control approaches for its vector mosquito. Parasit Vectors. 2014;7:258.CrossRefPubMedPubMedCentral
13.
Jamjoom GA, Azhar EI, Kao MA, Radadi RM. Seroepidemiology of Asymptomatic Dengue Virus Infection in Jeddah, Saudi Arabia. Virology (Auckl). 2016;7:1–7.
14.
Ahmed NH, Broor S. Comparison of NS1 antigen detection ELISA, real time RT-PCR and virus isolation for rapid diagnosis of dengue infection in acute phase. J Vector Borne Dis. 2014;51:194–9.PubMed
15.
16.
Walsh GM, Shih AW, Solh Z, Golder M, Schubert P, Fearon M, Sheffield WP. Blood-Borne Pathogens: A Canadian Blood Services Centre for Innovation Symposium. Transfus Med Rev. 2016;30:53–68.CrossRefPubMed
17.
Arellanos-Soto D, BdlC V, Mendoza-Tavera N, Ramos-Jimenez J, Cazares-Tamez R, Ortega-Soto A, Rivas-Estilla AM. Constant risk of dengue virus infection by blood transfusion in an endemic area in Mexico. Transfus Med. 2015;25:122–4.CrossRefPubMed
18.
19.
Sutherland MR, Simon AY, Serrano K, Schubert P, Acker JP, Pryzdial EL. Dengue virus persists and replicates during storage of platelet and red blood cell units. Transfusion. 2016;56:1129–37.CrossRefPubMed
20.
Anez G, Heisey DA, Chancey C, Fares RC, Espina LM, Souza KP, Teixeira-Carvalho A, Krysztof DE, Foster GA, Stramer SL, Rios M. Distribution of Dengue Virus Types 1 and 4 in Blood Components from Infected Blood Donors from Puerto Rico. PLoS Negl Trop Dis. 2016;10:e0004445.CrossRefPubMedPubMedCentral
21.
Ashshi AM. Serodetection of Dengue virus and its antibodies among blood donors in the western region of Saudi Arabia: a preliminary study. Blood Transfus. 2015;13:135–8.PubMedPubMedCentral
22.
De La Cruz Hernández SI, Reyes-del Valle J, Villegas-del Angel E, Ludert JE, del Angel RM. Dengue laboratory diagnosis: still some room for improvement. Futur Virol. 2015;10:845–57.CrossRef
23.
Ashshi AM, Batwa SA, Kutbi SY, Malibary FA, Batwa M, Refaat B. Prevalence of 7 sexually transmitted organisms by multiplex real-time PCR in Fallopian tube specimens collected from Saudi women with and without ectopic pregnancy. BMC Infect Dis. 2015;15:569.CrossRefPubMedPubMedCentral
24.
Alzahrani AG, Al Mazroa MA, Alrabeah AM, Ibrahim AM, Mokdad AH, Memish ZA. Geographical distribution and spatio-temporal patterns of dengue cases in Jeddah Governorate from 2006–2008. Trans R Soc Trop Med Hyg. 2013;107:23–9.CrossRefPubMed
25.
El-Kafrawy SA, Sohrab SS, Ela SA, Abd-Alla AM, Alhabbab R, Farraj SA, Othman NA, Hassan AM, Bergoin M, Klitting R, Charrel RN, Hashem AM, Madani TA, Azhar EI. Multiple Introductions of Dengue 2 Virus Strains into Saudi Arabia from 1992 to 2014. Vector Borne Zoonotic Dis. 2016;16:391–9.CrossRefPubMedPubMedCentral
26.
Organji SR, Abulreesh HH, Osman GEH. Circulation of Dengue Virus Serotypes in the City of Makkah, Saudi Arabia, as Determined by Reverse Transcription Polymerase Chain Reaction. Can J Infect Dis Med Microbiol. 2017;2017:5.
27.
Amarasinghe A, Letson GW. Dengue in the Middle East: a neglected, emerging disease of importance. Trans R Soc Trop Med Hyg. 2012;106:1–2.CrossRefPubMed
28.
29.
Saha K, Ghosh M, Firdaus R, Biswas A, Seth B, Bhattacharya D, Mukherjee K, Sadhukhan PC. Changing pattern of dengue virus serotypes circulating during 2008–2012 and reappearance of dengue serotype 3 may cause outbreak in Kolkata, India. J Med Virol. 2016;88:1697–702.CrossRefPubMed
30.
Suleman M, Lee HW, Zaidi SS, Alam MM, Nisar N, Aamir UB, Sharif S, Shaukat S, Khurshid A, Angez M, Umair M, Mujtaba G, Faryal R. Preliminary Seroepidemiological survey of dengue infections in Pakistan, 2009–2014. Infect Dis Poverty. 2017;6:48.CrossRefPubMedPubMedCentral
31.
Stramer SL, Linnen JM, Carrick JM, Foster GA, Krysztof DE, Zou S, Dodd RY, Tirado-Marrero LM, Hunsperger E, Santiago GA, Munoz-Jordan JL, Tomashek KM. Dengue viremia in blood donors identified by RNA and detection of dengue transfusion transmission during the 2007 dengue outbreak in Puerto Rico. Transfusion. 2012;52:1657–66.CrossRefPubMed
32.
Busch MP, Sabino EC, Brambilla D, Lopes ME, Capuani L, Chowdhury D, McClure C, Linnen JM, Prince H, Simmons G, Lee TH, Kleinman S, Custer B, International Component of the NRE, Donor Evaluation S, III. Duration of Dengue Viremia in Blood Donors and Relationships Between Donor Viremia, Infection Incidence and Clinical Case Reports During a Large Epidemic. J Infect Dis. 2016;214:49–54.CrossRefPubMed
33.
Abdel Gader AG, Osman AM, Al Gahtani FH, Farghali MN, Ramadan AH, Al-Momen AK. Attitude to blood donation in Saudi Arabia. Asian J Transfus Sci. 2011;5:121–6.CrossRefPubMedPubMedCentral
34.
Al-Johar AW, Al-Saud A, Abalkhail Y, Jawdat T, Al-Khamees S, Al-Thunayan F, Abdel-Gader AG. Why do-Saudi Women Refrain Donating Their Blood?-a Study on the Attitude, Belief and Motivation of Saudi Female University Students Towards Blood Donation. Clin Lab. 2016;62:771–9.CrossRefPubMed
35.
Metadata
Title
The prevalence of dengue virus serotypes in asymptomatic blood donors reveals the emergence of serotype 4 in Saudi Arabia
Author
Ahmed Mohamed Ashshi
Publication date
01-12-2017
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2017
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/s12985-017-0768-7

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