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Virology Journal
Published in: Virology Journal 1/2017

Open Access 01-12-2017 | Research

Transcriptome analysis of Cucumis sativus infected by Cucurbit chlorotic yellows virus

Authors: Xinyan Sun, Zhenyue Wang, Qinsheng Gu, Honglian Li, Weili Han, Yan Shi

Published in: Virology Journal | Issue 1/2017

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Abstract

Background

Cucurbit chlorotic yellows virus (CCYV) is a recently reported bipartite crinivirus that causes chlorotic leaf spots and yellowing symptoms on the leaves of cucurbit plants. The virus–host interaction of CCYV remains to be elucidated, and the influence of criniviruses on the host gene transcriptome requires analysis.

Methods

We used transcriptome sequencing to analyse the differentially expressed genes (DEGs) caused by CCYV infection.

Results

CCYV infection resulted in 865 DEGs. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis identified 67 pathways, and the three major enrichment pathways (according to the P-values) were photosynthesis-antenna proteins (KO00196), phenylalanine metabolism (KO00360a), and phenylpropanoid biosynthesis (KO00940). Of the 13 DEGs identified in phenylalanine metabolism, 11 genes encode disease resistance-related phenylalanine ammonia-lyase (PAL) genes. Using quantitative real-time PCR, we validated the differential expression of 12 genes.

Conclusions

Our study based on the CCYV–cucumber interaction provides comprehensive transcriptomic information, and will improve our understanding of host–crinivirus interactions.
Appendix
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Metadata
Title
Transcriptome analysis of Cucumis sativus infected by Cucurbit chlorotic yellows virus
Authors
Xinyan Sun
Zhenyue Wang
Qinsheng Gu
Honglian Li
Weili Han
Yan Shi
Publication date
01-12-2017
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2017
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/s12985-017-0690-z

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