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Open Access 01-12-2016 | Research

Cloning and rescue of the genome of Bombyx mori bidensovirus, and characterization of a recombinant virus

Authors: Panpan Zhang, Di Miao, Yahui Zhang, Meizi Wang, Zhaoyang Hu, Peng Lü, Qin Yao

Published in: Virology Journal | Issue 1/2016

Abstract

Background

Bombyx mori bidensovirus (BmBDV), which belongs to the Bidnaviridae family established by the International Committee on Taxonomy of Viruses in 2011, was the first bidensovirus identified in insects. The structure of BmBDV is similar to that of parvoviruses, while its replication is similar to that of adenoviruses. Although BmBDV has the potential to be used as a tool in biological pest control and as an expression vector, virus rescue has been a bottleneck in the application of this virus.

Methods

In this study, we constructed a full-length genomic clone of BmBDV and showed that its terminal structure was restored. A recombinant BmBDV that expressed the green fluorescence protein (GFP) gene was constructed. Then, BmN cells, which are an ovarian cell line, were co-transfected with the linearized genome using continuous culture and expanded cell culture methods.

Results

The results showed that the GFP gene was expressed successfully, and that cell lesions occurred in virus-infected cells. Furthermore, typical densonucleosis viruses were observed in reinfected silkworm larvae and larval midgut tissues infected by BmBDV, as evidenced by the emission of green fluorescence.

Conclusions

Overall, these results suggest that the virus could be rescued from the infected BmN cells after co-transfection with the linear full length virus genome.

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Title: Cloning and rescue of the genome of Bombyx mori bidensovirus, and characterization of a recombinant virus
Authors: Panpan Zhang
Di Miao
Yahui Zhang
Meizi Wang
Zhaoyang Hu
Peng Lü
Qin Yao
Publication date: 01-12-2016
Publisher: BioMed Central
Published in: Virology Journal / Issue 1/2016
Electronic ISSN: 1743-422X
DOI: https://doi.org/10.1186/s12985-016-0576-5

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