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Open Access 01-12-2016 | Short report

Differences in viral load among human respiratory syncytial virus genotypes in hospitalized children with severe acute respiratory infections in the Philippines

Authors: Francois Marie Ngako Kadji, Michiko Okamoto, Yuki Furuse, Raita Tamaki, Akira Suzuki, Irene Lirio, Clyde Dapat, Rungnapa Malasao, Mariko Saito, Gay Anne Granada Pedrera-Rico, Veronica Tallo, Socorro Lupisan, Mayuko Saito, Hitoshi Oshitani

Published in: Virology Journal | Issue 1/2016

Abstract

Background

Human respiratory syncytial virus (HRSV) is a leading viral etiologic agent of pediatric lower respiratory infections, including bronchiolitis and pneumonia. Two antigenic subgroups, HRSV-A and B, each contain several genotypes. While viral load may vary among HRSV genotypes and affect the clinical course of disease, data are scarce regarding the actual differences among genotypes. Therefore, this study estimated and compared viral load among NA1 and ON1 genotypes of HRSV-A and BA9 of HRSV-B. ON1 is a newly emerged genotype with a 72-nucleotide duplication in the G gene as observed previously with BA genotypes in HRSV-B.

Findings

Children <5 years of age with an initial diagnosis of severe or very severe pneumonia at a hospital in the Philippines from September 2012 to December 2013 were enrolled. HRSV genotypes were determined and the viral load measured from nasopharyngeal swabs (NPS). The viral load of HRSV genotype NA1 were significantly higher than those of ON1 and BA9. Regression analysis showed that both genotype NA1 and younger age were significantly associated with high HRSV viral load.

Conclusions

The viral load of NA1 was higher than that of ON1 and BA9 in NPS samples. HRSV genotypes may be associated with HRSV viral load. The reasons and clinical impacts of these differences in viral load among HRSV genotypes require further evaluation.

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Title: Differences in viral load among human respiratory syncytial virus genotypes in hospitalized children with severe acute respiratory infections in the Philippines
Authors: Francois Marie Ngako Kadji
Michiko Okamoto
Yuki Furuse
Raita Tamaki
Akira Suzuki
Irene Lirio
Clyde Dapat
Rungnapa Malasao
Mariko Saito
Gay Anne Granada Pedrera-Rico
Veronica Tallo
Socorro Lupisan
Mayuko Saito
Hitoshi Oshitani
Publication date: 01-12-2016
Publisher: BioMed Central
Published in: Virology Journal / Issue 1/2016
Electronic ISSN: 1743-422X
DOI: https://doi.org/10.1186/s12985-016-0565-8

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