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Virology Journal
Published in: Virology Journal 1/2016

Open Access 01-12-2016 | Research

miR-124 attenuates Japanese encephalitis virus replication by targeting DNM2

Authors: Songbai Yang, Yue Pei, Xinyun Li, Shuhong Zhao, Mengjin Zhu, Ayong Zhao

Published in: Virology Journal | Issue 1/2016

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Abstract

Background

Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus that causes acute viral encephalitis in humans. Pigs are important amplifier hosts of JEV. Emerging evidence indicates that host microRNAs (miRNAs) play key roles in modulating viral infection and pathogenesis. However, mechanistic studies delineating the roles of miRNAs in regulating host-JEV interactions remain scarce.

Results

In this study, we demonstrated that miR-124 inhibited JEV replication in porcine kidney epithelial PK15 cells. Furthermore, using bioinformatics tools, we identified dynamin2 (DNM2), a GTPase responsible for vesicle scission, as a target of miR-124. Small interfering RNA (siRNA) depletion studies inicated that dynamin2 was required for efficient JEV replication. We also demonstrated that upregulation of miR-124 expression corresponded to decreased expression of its target, DNM2, in the JEV-infected PK15 cells.

Conclusions

Overall, these results suggest the importance of miR-124 in modulating JEV replication and provide a scientific basis for using cellular miRNAs in anti-JEV therapies.
Appendix
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Metadata
Title
miR-124 attenuates Japanese encephalitis virus replication by targeting DNM2
Authors
Songbai Yang
Yue Pei
Xinyun Li
Shuhong Zhao
Mengjin Zhu
Ayong Zhao
Publication date
01-12-2016
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2016
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/s12985-016-0562-y

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