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Virology Journal

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Open Access 01-12-2015 | Research

The efficacy of zinc finger antiviral protein against hepatitis B virus transcription and replication in tansgenic mouse model

Authors: En-Qiang Chen, Jie Dai, Lang Bai, Hong Tang

Published in: Virology Journal | Issue 1/2015

Abstract

Aim

The zinc finger antiviral protein (ZAP) is a mammalian host restriction factor, and it could inhibit HBV RNA synthesis in vitro experiments. However, the role of ZAP against HBV in vivo environment is unclear. This study aimed to investigate whether ZAP could act against HBV transcription and replication in ZAP tansgenic mouse model.

Methods

HBV-replication-competent plasmid pHBV4.1 was transferred to ZAP transgenic ICR mouse via the tail vein using a hydrodynamic in vivo transfection procedure, and ICR mouse were used as controls. HBV RNA and HBV DNA replication intermediates in the liver were respectively analyzed by Northern blotting and Southern blotting. The expression of hepatitis B surface antigen (HBsAg) and hepatitis B core antigen (HBcAg) in the liver tissue was detected by immunohistochemical staining.

Results

As compared to ICR control mouse, the levels of 3.5 kb mRNA in ZAP transgenic mouse were decreased by 8.4%; while the level of HBV DNA replication intermediates was decreased by 82%. In addition, the expression of HBsAg and HBcAg in ZAP transgenic mouse liver were both significantly less than that of ICR control mouse.

Conclusions

Our findings suggest that ZAP could inhibit HBV replication in vivo in mice, which offers a new target for anti-HBV drug development.

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Title: The efficacy of zinc finger antiviral protein against hepatitis B virus transcription and replication in tansgenic mouse model
Authors: En-Qiang Chen
Jie Dai
Lang Bai
Hong Tang
Publication date: 01-12-2015
Publisher: BioMed Central
Published in: Virology Journal / Issue 1/2015
Electronic ISSN: 1743-422X
DOI: https://doi.org/10.1186/s12985-015-0245-0

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Abstract

Aim

Methods

Results

Conclusions

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