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Open Access 01-12-2014 | Short report

E5564 inhibits immunosuppressive cytokine IL-10 induction promoted by HIV-1 Tat protein

Authors: Elmostafa Bahraoui, Laurence Briant, Nathalie Chazal

Published in: Virology Journal | Issue 1/2014

Abstract

Background

In HIV-1 infected patients, production of interleukin-10 (IL-10), a highly immunosuppressive cytokine, is associated with progression of infection toward AIDS. HIV-1 Tat protein, by interacting with TLR4-MD2 at the membrane level, induces IL-10 production by primary human monocytes and macrophages. In the present study we evaluated the effect of the TLR4 antagonist Eritoran tetrasodium (E5564) on HIV-1 Tat-induced IL-10 production.

Findings

Here, we confirm that the recombinant HIV-1 Tat protein and the GST-Tat 1–45 fusion protein efficiently stimulate IL-10 production by primary monocytes and macrophages and that this stimulation is inhibited by blocking anti-TLR4 mAbs. We show that a similar inhibition is observed by preincubating the cells with the TLR4 antagonist E5564.

Conclusion

This study provides compelling data showing for the first time that the TLR4 antagonist E5564 inhibits the immunosuppressive cytokine IL-10 production by primary human monocytes and macrophages incubated in the presence of HIV-1 Tat protein.

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Title: E5564 inhibits immunosuppressive cytokine IL-10 induction promoted by HIV-1 Tat protein
Authors: Elmostafa Bahraoui
Laurence Briant
Nathalie Chazal
Publication date: 01-12-2014
Publisher: BioMed Central
Published in: Virology Journal / Issue 1/2014
Electronic ISSN: 1743-422X
DOI: https://doi.org/10.1186/s12985-014-0214-z

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