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Journal of Neuroinflammation
Published in: Journal of Neuroinflammation 1/2017

Open Access 01-12-2017 | Research

Grape seed-derived procyanidins alleviate gout pain via NLRP3 inflammasome suppression

Authors: Hai-Jiao Liu, Xiu-Xiu Pan, Bing-Qian Liu, Xuan Gui, Liang Hu, Chun-Yi Jiang, Yuan Han, Yi-Xin Fan, Yu-Lin Tang, Wen-Tao Liu

Published in: Journal of Neuroinflammation | Issue 1/2017

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Abstract

Background

Gout is one of the common inflammatory arthritis which affects many people for inflicting unbearable pain. Macrophage-mediated inflammation plays an important role in gout. The uptake of monosodium urate (MSU) crystals by macrophages can lead to activation of NOD-like receptors containing a PYD 3 (NLRP3) inflammasome, thus accelerating interleukin (IL)-1β production. Reactive oxygen species (ROS) promoted development of the inflammatory process through NLRP3 inflammasome. Our study aimed to find a food-derived compound to attenuate gout pain via the specific inhibition of the NLRP3 inflammasome in macrophages.

Methods

CD-1 mice were used to evaluate the degree of pain and the swelling dimension of joints after an intra-articular (IA) MSU injection in the ankle. The murine macrophage cell line Raw 264.7 was used to investigate the effects of procyanidins and the mechanism underlying such effects. Histological analysis was used to measure the infiltration of inflammatory cells. ROS produced from Raw 264.7 cells were evaluated by flow cytometry. Cell signaling was measured by Western blot assay and immunofluorescence.

Results

Procyanidins significantly attenuated gout pain and suppressed ankle swelling. Procyanidins also inhibited MSU-induced activation of the NLRP3 inflammasome and increase of IL-1β. Furthermore, procyanidins decreased ROS levels in Raw 264.7 cells.

Conclusions

Suppression of the NLRP3 inflammasome in macrophages contributes to the amelioration of gout pain by procyanidins.
Appendix
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Metadata
Title
Grape seed-derived procyanidins alleviate gout pain via NLRP3 inflammasome suppression
Authors
Hai-Jiao Liu
Xiu-Xiu Pan
Bing-Qian Liu
Xuan Gui
Liang Hu
Chun-Yi Jiang
Yuan Han
Yi-Xin Fan
Yu-Lin Tang
Wen-Tao Liu
Publication date
01-12-2017
Publisher
BioMed Central
Published in
Journal of Neuroinflammation / Issue 1/2017
Electronic ISSN: 1742-2094
DOI
https://doi.org/10.1186/s12974-017-0849-y

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