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Published in: Journal of the International Society of Sports Nutrition 1/2018

Open Access 01-12-2018 | Research article

Effects of lemon verbena extract (Recoverben®) supplementation on muscle strength and recovery after exhaustive exercise: a randomized, placebo-controlled trial

Authors: Sybille Buchwald-Werner, Ioanna Naka, Manfred Wilhelm, Elivra Schütz, Christiane Schoen, Claudia Reule

Published in: Journal of the International Society of Sports Nutrition | Issue 1/2018

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Abstract

Background

Exhaustive exercise causes muscle damage accompanied by oxidative stress and inflammation leading to muscle fatigue and muscle soreness. Lemon verbena leaves, commonly used as tea and refreshing beverage, demonstrated antioxidant and anti-inflammatory properties. The aim of this study was to investigate the effects of a proprietary lemon verbena extract (Recoverben®) on muscle strength and recovery after exhaustive exercise in comparison to a placebo product.

Methods

The study was performed as a randomized, placebo-controlled, double-blind study with parallel design. Forty-four healthy males and females, which were 22–50 years old and active in sports, were randomized to 400 mg lemon verbena extract once daily or placebo. The 15 days intervention was divided into 10 days supplementation prior to the exhaustive exercise day (intensive jump-protocol), one day during the test and four days after. Muscle strength (MVC), muscle damage (CK), oxidative stress (GPx), inflammation (IL6) and volunteer-reported muscle soreness intensity were assessed pre and post exercise.

Results

Participants in the lemon verbena group benefited from less muscle damage as well as faster and full recovery. Compared to placebo, lemon verbena extract receiving participants had significantly less exercise-related loss of muscle strength (p = 0.0311) over all timepoints, improved glutathione peroxidase activity by trend (p = 0.0681) and less movement induced pain (p = 0.0788) by trend. Creatine kinase and IL-6 didn’t show significant discrimmination between groups.

Conclusion

Lemon verbena extract (Recoverben®) has been shown to be a safe and well-tolerated natural sports ingredient, by reducing muscle damage after exhaustive exercise.

Trial registration

The trial was registered in the clinical trials registry (clinical trial.gov NCT02923102). Registered 28 September 2016
Literature
1.
go back to reference Paulsen G, Mikkelsen UR, Raastad T, Peake JM. Leucocytes, cytokines and satellite cells: what role do they play in muscle damage and regeneration following eccentric exercise? Exerc Immunol Rev. 2012;18:42-97. Paulsen G, Mikkelsen UR, Raastad T, Peake JM. Leucocytes, cytokines and satellite cells: what role do they play in muscle damage and regeneration following eccentric exercise? Exerc Immunol Rev. 2012;18:42-97.
2.
go back to reference McLeay Y, Barnes MJ, Mundel T, Hurst SM, Hurst RD, Stannard SR. Effect of New Zealand blueberry consumption on recovery from eccentric exercise-induced muscle damage. J Int Soc Sports Nutr. 2012;9:19.CrossRefPubMedPubMedCentral McLeay Y, Barnes MJ, Mundel T, Hurst SM, Hurst RD, Stannard SR. Effect of New Zealand blueberry consumption on recovery from eccentric exercise-induced muscle damage. J Int Soc Sports Nutr. 2012;9:19.CrossRefPubMedPubMedCentral
3.
go back to reference Meamarbashi A. Herbs and natural supplements in the prevention and treatment of delayed-onset muscle soreness. Avicenna J Phytomed. 2017;7:16–26.PubMedPubMedCentral Meamarbashi A. Herbs and natural supplements in the prevention and treatment of delayed-onset muscle soreness. Avicenna J Phytomed. 2017;7:16–26.PubMedPubMedCentral
6.
go back to reference Tartibian B, Maleki BH, Abbasi A. Omega-3 fatty acids supplementation attenuates inflammatory markers after eccentric exercise in untrained men. Clin J Sport Med. 2011;21:131–7.CrossRefPubMed Tartibian B, Maleki BH, Abbasi A. Omega-3 fatty acids supplementation attenuates inflammatory markers after eccentric exercise in untrained men. Clin J Sport Med. 2011;21:131–7.CrossRefPubMed
8.
go back to reference Cases J, Romain C, Marin-Pagan C, Chung LH, Rubio-Perez JM, Laurent C, et al. Supplementation with a polyphenol-rich extract, PerfLoad(R), improves physical performance during high-intensity exercise: a randomized, double blind, crossover trial. Nutrients. 2017;9:E421.CrossRefPubMed Cases J, Romain C, Marin-Pagan C, Chung LH, Rubio-Perez JM, Laurent C, et al. Supplementation with a polyphenol-rich extract, PerfLoad(R), improves physical performance during high-intensity exercise: a randomized, double blind, crossover trial. Nutrients. 2017;9:E421.CrossRefPubMed
9.
go back to reference Connolly DA, McHugh MP, Padilla-Zakour OI, Carlson L, Sayers SP. Efficacy of a tart cherry juice blend in preventing the symptoms of muscle damage. Br J Sports Med. 2006;40:679–83.CrossRefPubMedPubMedCentral Connolly DA, McHugh MP, Padilla-Zakour OI, Carlson L, Sayers SP. Efficacy of a tart cherry juice blend in preventing the symptoms of muscle damage. Br J Sports Med. 2006;40:679–83.CrossRefPubMedPubMedCentral
10.
go back to reference Trombold JR, Barnes JN, Critchley L, Coyle EF. Ellagitannin consumption improves strength recovery 2-3 d after eccentric exercise. Med Sci Sports Exerc. 2010;42:493–8.CrossRefPubMed Trombold JR, Barnes JN, Critchley L, Coyle EF. Ellagitannin consumption improves strength recovery 2-3 d after eccentric exercise. Med Sci Sports Exerc. 2010;42:493–8.CrossRefPubMed
11.
go back to reference Lenoir L, Rossary A, Joubert-Zakeyh J, Vergnaud-Gauduchon J, Farges MC, Fraisse D, et al. Lemon verbena infusion consumption attenuates oxidative stress in dextran sulfate sodium-induced colitis in the rat. Dig Dis Sci. 2011;56:3534–45.CrossRefPubMed Lenoir L, Rossary A, Joubert-Zakeyh J, Vergnaud-Gauduchon J, Farges MC, Fraisse D, et al. Lemon verbena infusion consumption attenuates oxidative stress in dextran sulfate sodium-induced colitis in the rat. Dig Dis Sci. 2011;56:3534–45.CrossRefPubMed
12.
go back to reference Bilia AR, Giomi M, Innocenti M, Gallori S, Vincieri FF. HPLC-DAD-ESI-MS analysis of the constituents of aqueous preparations of verbena and lemon verbena and evaluation of the antioxidant activity. J Pharm Biomed Anal. 2008;46:463–70.CrossRefPubMed Bilia AR, Giomi M, Innocenti M, Gallori S, Vincieri FF. HPLC-DAD-ESI-MS analysis of the constituents of aqueous preparations of verbena and lemon verbena and evaluation of the antioxidant activity. J Pharm Biomed Anal. 2008;46:463–70.CrossRefPubMed
13.
go back to reference Quirantes-Pine R, Funes L, Micol V, Segura-Carretero A, Fernandez-Gutierrez A. High-performance liquid chromatography with diode array detection coupled to electrospray time-of-flight and ion-trap tandem mass spectrometry to identify phenolic compounds from a lemon verbena extract. J Chromatogr A. 2009;1216:5391–7.CrossRefPubMed Quirantes-Pine R, Funes L, Micol V, Segura-Carretero A, Fernandez-Gutierrez A. High-performance liquid chromatography with diode array detection coupled to electrospray time-of-flight and ion-trap tandem mass spectrometry to identify phenolic compounds from a lemon verbena extract. J Chromatogr A. 2009;1216:5391–7.CrossRefPubMed
14.
go back to reference Felgines C, Fraisse D, Besson C, Vasson MP, Texier O. Bioavailability of lemon verbena (Aloysia Triphylla) polyphenols in rats: impact of colonic inflammation. Br J Nutr. 2014;111:1773–81.CrossRefPubMed Felgines C, Fraisse D, Besson C, Vasson MP, Texier O. Bioavailability of lemon verbena (Aloysia Triphylla) polyphenols in rats: impact of colonic inflammation. Br J Nutr. 2014;111:1773–81.CrossRefPubMed
15.
go back to reference Portmann E, Nigro MM, Reides CG, Llesuy S, Ricco RA, Wagner ML, et al. Aqueous extracts of Lippia Turbinata and Aloysia Citriodora (Verbenaceae): assessment of antioxidant capacity and DNA damage. Int J Toxicol. 2012;31:192–202.CrossRefPubMed Portmann E, Nigro MM, Reides CG, Llesuy S, Ricco RA, Wagner ML, et al. Aqueous extracts of Lippia Turbinata and Aloysia Citriodora (Verbenaceae): assessment of antioxidant capacity and DNA damage. Int J Toxicol. 2012;31:192–202.CrossRefPubMed
16.
go back to reference Abderrahim F, Estrella S, Susin C, Arribas SM, Gonzalez MC, Condezo-Hoyos L. The antioxidant activity and thermal stability of lemon verbena (Aloysia Triphylla) infusion. J Med Food. 2011;14:517–27.CrossRefPubMed Abderrahim F, Estrella S, Susin C, Arribas SM, Gonzalez MC, Condezo-Hoyos L. The antioxidant activity and thermal stability of lemon verbena (Aloysia Triphylla) infusion. J Med Food. 2011;14:517–27.CrossRefPubMed
17.
go back to reference Ponce-Monter H, Fernandez-Martinez E, Ortiz MI, Ramirez-Montiel ML, Cruz-Elizalde D, Perez-Hernandez N, et al. Spasmolytic and anti-inflammatory effects of Aloysia Triphylla and citral, in vitro and in vivo studies. J Smooth Muscle Res. 2010;46:309–19.CrossRefPubMed Ponce-Monter H, Fernandez-Martinez E, Ortiz MI, Ramirez-Montiel ML, Cruz-Elizalde D, Perez-Hernandez N, et al. Spasmolytic and anti-inflammatory effects of Aloysia Triphylla and citral, in vitro and in vivo studies. J Smooth Muscle Res. 2010;46:309–19.CrossRefPubMed
18.
go back to reference Choupani M, Arabshahi S, Alami M. Antioxidant properties of various solvent extracts of lemon verbena (Lippia Citriodora) leaves. Int J Adv Biol Biomed Res. 2014;2 Choupani M, Arabshahi S, Alami M. Antioxidant properties of various solvent extracts of lemon verbena (Lippia Citriodora) leaves. Int J Adv Biol Biomed Res. 2014;2
19.
go back to reference Funes L, Carrera-Quintanar L, Cerdan-Calero M, Ferrer MD, Drobnic F, Pons A, et al. Effect of lemon verbena supplementation on muscular damage markers, proinflammatory cytokines release and neutrophils' oxidative stress in chronic exercise. Eur J Appl Physiol. 2011;111:695–705.CrossRefPubMed Funes L, Carrera-Quintanar L, Cerdan-Calero M, Ferrer MD, Drobnic F, Pons A, et al. Effect of lemon verbena supplementation on muscular damage markers, proinflammatory cytokines release and neutrophils' oxidative stress in chronic exercise. Eur J Appl Physiol. 2011;111:695–705.CrossRefPubMed
20.
go back to reference Mauriz E, Vallejo D, Tunon MJ, Rodriguez-Lopez JM, Rodriguez-Perez R, Sanz-Gomez J, et al. Effects of dietary supplementation with lemon verbena extracts on serum inflammatory markers of multiple sclerosis patients. Nutr Hosp. 2014;31:764–71.PubMed Mauriz E, Vallejo D, Tunon MJ, Rodriguez-Lopez JM, Rodriguez-Perez R, Sanz-Gomez J, et al. Effects of dietary supplementation with lemon verbena extracts on serum inflammatory markers of multiple sclerosis patients. Nutr Hosp. 2014;31:764–71.PubMed
21.
go back to reference Carrera-Quintanar L, Funes L, Vicente-Salar N, Blasco-Lafarga C, Pons A, Micol V, et al. Effect of polyphenol supplements on redox status of blood cells: a randomized controlled exercise training trial. Eur J Nutr. 2015;54:1081–93.CrossRefPubMed Carrera-Quintanar L, Funes L, Vicente-Salar N, Blasco-Lafarga C, Pons A, Micol V, et al. Effect of polyphenol supplements on redox status of blood cells: a randomized controlled exercise training trial. Eur J Nutr. 2015;54:1081–93.CrossRefPubMed
22.
go back to reference Caturla N, Funes L, Perez-Fons L, Micol V. A randomized, double-blinded, placebo-controlled study of the effect of a combination of lemon verbena extract and fish oil omega-3 fatty acid on joint management. J Altern Complement Med. 2011;17:1051–63.CrossRefPubMedPubMedCentral Caturla N, Funes L, Perez-Fons L, Micol V. A randomized, double-blinded, placebo-controlled study of the effect of a combination of lemon verbena extract and fish oil omega-3 fatty acid on joint management. J Altern Complement Med. 2011;17:1051–63.CrossRefPubMedPubMedCentral
23.
go back to reference Mestre-Alfaro A, Ferrer MD, Sureda A, Tauler P, Martinez E, Bibiloni MM, et al. Phytoestrogens enhance antioxidant enzymes after swimming exercise and modulate sex hormone plasma levels in female swimmers. Eur J Appl Physiol. 2011;111:2281–94.CrossRefPubMed Mestre-Alfaro A, Ferrer MD, Sureda A, Tauler P, Martinez E, Bibiloni MM, et al. Phytoestrogens enhance antioxidant enzymes after swimming exercise and modulate sex hormone plasma levels in female swimmers. Eur J Appl Physiol. 2011;111:2281–94.CrossRefPubMed
24.
go back to reference Carrera-Quintanar L, Funes L, Viudes E, Tur J, Micol V, Roche E, et al. Antioxidant effect of lemon verbena extracts in lymphocytes of university students performing aerobic training program. Scand J Med Sci Sports. 2012;22:454–61.CrossRefPubMed Carrera-Quintanar L, Funes L, Viudes E, Tur J, Micol V, Roche E, et al. Antioxidant effect of lemon verbena extracts in lymphocytes of university students performing aerobic training program. Scand J Med Sci Sports. 2012;22:454–61.CrossRefPubMed
26.
go back to reference Buchwald-Werner S, Naka I, Dressler D, Reule C, Schoen C. Aloysia Citriodora extract targeting sports nutrition. In vitro study on anti-inflammatory effects by cyclooxygenases inhibition. Agro Food ind Hi Tech. 2017;28:42–4. Buchwald-Werner S, Naka I, Dressler D, Reule C, Schoen C. Aloysia Citriodora extract targeting sports nutrition. In vitro study on anti-inflammatory effects by cyclooxygenases inhibition. Agro Food ind Hi Tech. 2017;28:42–4.
27.
go back to reference Ahmed M, Qadir MA, Hameed A, Imran M, Muddassar M. Screening of curcumin-derived isoxazole, pyrazoles, and pyrimidines for their anti-inflammatory, antinociceptive, and cyclooxygenase-2 inhibition. Chem Biol Drug Des. 2018;91:338–43. Ahmed M, Qadir MA, Hameed A, Imran M, Muddassar M. Screening of curcumin-derived isoxazole, pyrazoles, and pyrimidines for their anti-inflammatory, antinociceptive, and cyclooxygenase-2 inhibition. Chem Biol Drug Des. 2018;91:338–43.
28.
go back to reference Delecroix B, Abaidia AE, Leduc C, Dawson B, Dupont G. Curcumin and Piperine supplementation and recovery following exercise induced muscle damage: a randomized controlled trial. J Sports Sci Med. 2017;16:147–53.PubMedPubMedCentral Delecroix B, Abaidia AE, Leduc C, Dawson B, Dupont G. Curcumin and Piperine supplementation and recovery following exercise induced muscle damage: a randomized controlled trial. J Sports Sci Med. 2017;16:147–53.PubMedPubMedCentral
29.
go back to reference IPAQ Group. International physical activity questionnaire. 2016. Ref Type: Internet Communication. IPAQ Group. International physical activity questionnaire. 2016. Ref Type: Internet Communication.
30.
go back to reference Hug B. Validierung und Weiterentwicklung eines Kurzfragebogens zur Beurteilung des Ernährungsmusters. Institut für Biologische Chemie und Ernährungswissenschaft Prof Dr med H K Biesalski: Diplomarbeit; 2006. Hug B. Validierung und Weiterentwicklung eines Kurzfragebogens zur Beurteilung des Ernährungsmusters. Institut für Biologische Chemie und Ernährungswissenschaft Prof Dr med H K Biesalski: Diplomarbeit; 2006.
33.
go back to reference Howatson G, Hoad M, Goodall S, Tallent J, Bell PG, French DN. Exercise-induced muscle damage is reduced in resistance-trained males by branched chain amino acids: a randomized, double-blind, placebo controlled study. J Int Soc Sports Nutr. 2012;9:20. Howatson G, Hoad M, Goodall S, Tallent J, Bell PG, French DN. Exercise-induced muscle damage is reduced in resistance-trained males by branched chain amino acids: a randomized, double-blind, placebo controlled study. J Int Soc Sports Nutr. 2012;9:20.
34.
go back to reference Borg GA. Psychophysical bases of perceived exertion. Med Sci Sports Exerc. 1982;14:377–81.PubMed Borg GA. Psychophysical bases of perceived exertion. Med Sci Sports Exerc. 1982;14:377–81.PubMed
35.
go back to reference Marzin T, Lorkowski G, Reule C, Rau S, Pabst E, Vester JC. Effects of a systemic enzyme therapy in healthy active adults after exhaustive eccentric exercise: a randomised, two-stage, double-blinded, placebo-controlled trial. BMJ Open Sport Exerc Med. 2017;2:e000191. Marzin T, Lorkowski G, Reule C, Rau S, Pabst E, Vester JC. Effects of a systemic enzyme therapy in healthy active adults after exhaustive eccentric exercise: a randomised, two-stage, double-blinded, placebo-controlled trial. BMJ Open Sport Exerc Med. 2017;2:e000191.
36.
37.
go back to reference Stolle D, Rick W. An improved method for the determination of Creatine kinase activity in serum. J Clin Chem Clin Biochem. 1976;14:239–44.PubMed Stolle D, Rick W. An improved method for the determination of Creatine kinase activity in serum. J Clin Chem Clin Biochem. 1976;14:239–44.PubMed
38.
39.
go back to reference Brigelius-Flohe R. Tissue-specific functions of individual glutathione peroxidases. Free Radic Biol Med. 1999;27:951–65.CrossRefPubMed Brigelius-Flohe R. Tissue-specific functions of individual glutathione peroxidases. Free Radic Biol Med. 1999;27:951–65.CrossRefPubMed
40.
go back to reference Reihmane D, Dela F. Interleukin-6: possible biological roles during exercise. Eur J Sport Sci. 2014;14:242-50. Reihmane D, Dela F. Interleukin-6: possible biological roles during exercise. Eur J Sport Sci. 2014;14:242-50.
41.
go back to reference Pereira Panza VS, Diefenthaeler F, da Silva EL. Benefits of dietary phytochemical supplementation on eccentric exercise-induced muscle damage: is including antioxidants enough? Nutrition. 2015;31:1072–82.CrossRefPubMed Pereira Panza VS, Diefenthaeler F, da Silva EL. Benefits of dietary phytochemical supplementation on eccentric exercise-induced muscle damage: is including antioxidants enough? Nutrition. 2015;31:1072–82.CrossRefPubMed
42.
go back to reference Peternelj TT, Coombes JS. Antioxidant supplementation during exercise training: beneficial or detrimental? Sports Med. 2011;41:1043–69.CrossRefPubMed Peternelj TT, Coombes JS. Antioxidant supplementation during exercise training: beneficial or detrimental? Sports Med. 2011;41:1043–69.CrossRefPubMed
43.
go back to reference Alshammari E, Shafi S, Nurmi-Lawton J, Taylor A, Lanham-New S, Ferns G. Altered antioxidant and trace-element status in adolescent female gymnasts. Int J Sport Nutr Exerc Metab. 2010;20:291–8.CrossRefPubMed Alshammari E, Shafi S, Nurmi-Lawton J, Taylor A, Lanham-New S, Ferns G. Altered antioxidant and trace-element status in adolescent female gymnasts. Int J Sport Nutr Exerc Metab. 2010;20:291–8.CrossRefPubMed
44.
go back to reference Niess AM, Simon P. Response and adaptation of skeletal muscle to exercise--the role of reactive oxygen species. Front Biosci. 2007;12:4826–38.CrossRefPubMed Niess AM, Simon P. Response and adaptation of skeletal muscle to exercise--the role of reactive oxygen species. Front Biosci. 2007;12:4826–38.CrossRefPubMed
45.
go back to reference Steinbacher P, Eckl P. Impact of oxidative stress on exercising skeletal muscle. Biomol Ther. 2015;5:356–77. Steinbacher P, Eckl P. Impact of oxidative stress on exercising skeletal muscle. Biomol Ther. 2015;5:356–77.
46.
go back to reference Peake JM, Neubauer O, Della Gatta PA, Nosaka K. Muscle damage and inflammation during recovery from exercise. J Appl Physiol (1985). 2017;122:559–70.CrossRef Peake JM, Neubauer O, Della Gatta PA, Nosaka K. Muscle damage and inflammation during recovery from exercise. J Appl Physiol (1985). 2017;122:559–70.CrossRef
49.
go back to reference Braakhuis AJ, Hopkins WG. Impact of dietary antioxidants on sport performance: a review. Sports Med. 2015;45:939–55.CrossRefPubMed Braakhuis AJ, Hopkins WG. Impact of dietary antioxidants on sport performance: a review. Sports Med. 2015;45:939–55.CrossRefPubMed
Metadata
Title
Effects of lemon verbena extract (Recoverben®) supplementation on muscle strength and recovery after exhaustive exercise: a randomized, placebo-controlled trial
Authors
Sybille Buchwald-Werner
Ioanna Naka
Manfred Wilhelm
Elivra Schütz
Christiane Schoen
Claudia Reule
Publication date
01-12-2018
Publisher
BioMed Central
DOI
https://doi.org/10.1186/s12970-018-0208-0

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