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Open Access 01-12-2021 | Short communication

The Spin1 interactor, Spindoc, is dispensable for meiotic division, but essential for haploid spermatid development in mice

Authors: Xue Jiang, Xiaoli Zhu, Yu Cheng, Muhammad Azhar, Xuemei Xing, Wenqing Li, Yuzhu Cao, Qinghua Shi, Jianqiang Bao

Published in: Reproductive Biology and Endocrinology | Issue 1/2021

Abstract

In mammals, germline development undergoes dramatic morphological and molecular changes and is epigenetically subject to intricate yet exquisite regulation. Which epigenetic players and how they participate in the germline developmental process are not fully characterized. Spin1 is a multifunctional epigenetic protein reader that has been shown to recognize H3 “K4me3-R8me2a” histone marks, and more recently the non-canonical bivalent H3 “K4me3-K9me3/2” marks as well. As a robust Spin1-interacting cofactor, Spindoc has been identified to enhance the binding of Spin1 to its substrate histone marks, thereby modulating the downstream signaling; However, the physiological role of Spindoc in germline development is unknown. We generated two Spindoc knockout mouse models through CRISPR/Cas9 strategy, which revealed that Spindoc is specifically required for haploid spermatid development, but not essential for meiotic divisions in spermatocytes. This study unveiled a new epigenetic player that participates in haploid germline development.

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Title: The Spin1 interactor, Spindoc, is dispensable for meiotic division, but essential for haploid spermatid development in mice
Authors: Xue Jiang
Xiaoli Zhu
Yu Cheng
Muhammad Azhar
Xuemei Xing
Wenqing Li
Yuzhu Cao
Qinghua Shi
Jianqiang Bao
Publication date: 01-12-2021
Publisher: BioMed Central
Published in: Reproductive Biology and Endocrinology / Issue 1/2021
Electronic ISSN: 1477-7827
DOI: https://doi.org/10.1186/s12958-021-00828-8

At a glance: The STEP trials

Springer Medicine

The Spin1 interactor, Spindoc, is dispensable for meiotic division, but essential for haploid spermatid development in mice

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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