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Journal of Inflammation

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Open Access 01-12-2018 | Research

The role of N-glycosylation of CD200-CD200R1 interaction in classical microglial activation

Authors: Chao Liu, Yifen Shen, Ying Tang, Yongchun Gu

Published in: Journal of Inflammation | Issue 1/2018

Abstract

Background

Microglial inflammatory activation is the common feature of the central nervous system (CNS) diseases. Microglia can be activated and particularly polarized toward a dual role in the injured CNS. The CD200 receptor 1 (CD200R1) inhibits inflammatory microglia activation as illustrated by studies. Publications show abnormal activation of microglia secondary to the deficient inhibit of CD200-CD200R interaction. In the present study, we established a neuronal-microglia co-culture system to investigate the association between CD200R1 engagement and classical microglial activation. We analyzed the glycosylation of CD200R1 and the CD200 binding. Secretion of pro-inflammatory cytokines were measured.

Results

CD200R1 was N-glycosylated at Asparagine 44 (Asn44, N44). Mutation of this site disrupted CD200-CD200R1 interaction and up-regulated the expression of cytokines iNOS, CD86, IL-1β and TNF-α.

Conclusion

N44 of CD200R1 is a significant binding site for CD200-CD200R1 interaction and play a critical role in the maintenance of microglia. The N-glycosylation of CD200R1 could serve as a therapeutic agent for CNS inflammation.

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Title: The role of N-glycosylation of CD200-CD200R1 interaction in classical microglial activation
Authors: Chao Liu
Yifen Shen
Ying Tang
Yongchun Gu
Publication date: 01-12-2018
Publisher: BioMed Central
Published in: Journal of Inflammation / Issue 1/2018
Electronic ISSN: 1476-9255
DOI: https://doi.org/10.1186/s12950-018-0205-8

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Conclusion

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