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Journal of Inflammation

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Open Access 01-12-2015 | Research

The protective effects of chronic intermittent hypobaric hypoxia pretreatment against collagen-induced arthritis in rats

Authors: Min Shi, Fang Cui, Ai-Jing Liu, Hui-Jie Ma, Ming Cheng, Shu-Xia Song, Fang Yuan, De-Pei Li, Yi Zhang

Published in: Journal of Inflammation | Issue 1/2015

Abstract

Objective

To explore the immunological mechanisms underlying the effect of chronic intermittent hypobaric hypoxia (CIHH) pretreatment on collagen-induced arthritis (CIA) in rat.

Methods

Fifty-four adult male Sprague–Dawley rats were used in the experiment. Arthritis in CIA rats (n=18) was induced by injection of collagen. The CIHH+CIA rats (n=18) were treated with CIHH (simulated 3000 m altitude, 5 hours per day for 28 days, PO₂=108.8 mmHg) before CIA. The control rats (n=18) were not given any treatment.

Results

(1) Incidence rate of CIA in CIHH+CIA rats was significantly lower than that in CIA rats (P<0.05). (2) The paw thickness and arthritis index (AI) value in CIHH+CIA rats were lower than those in CIA rats (P<0.05). (3) The hyperplasia with inflammatory infiltration in synovial tissue of joints in CIHH+CIA rats was much alleviative compared with CIA rats. (4) TNF-α, IFN-γ, IL-4 and IL-17 in synovial tissue of joint and serum in CIHH+CIA rats were decreased compared with CIA rats (P<0.05). (5) The number of CD4-positive T-lymphocytes and the ratio of CD4/CD8 T-lymphocytes in peripheral blood in CIHH+CIA rats were lower than those in CIA rats (P<0.05). (6) The protein expression of HIF-1α and NF-κB in synovial tissue of joint in CIHH+CIA rats was decreased compared with CIA rats (P<0.05).

Conclusion

CIHH pretreatment has a protective effect against collagen-induced arthritis in rat through down-regulation of HIF-1α and NF-κB, inhibition of inflammatory cytokines TNF-α and IL-17, and balance in CD4/CD8 and Th1/Th2 T lymphocytes.

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Title: The protective effects of chronic intermittent hypobaric hypoxia pretreatment against collagen-induced arthritis in rats
Authors: Min Shi
Fang Cui
Ai-Jing Liu
Hui-Jie Ma
Ming Cheng
Shu-Xia Song
Fang Yuan
De-Pei Li
Yi Zhang
Publication date: 01-12-2015
Publisher: BioMed Central
Published in: Journal of Inflammation / Issue 1/2015
Electronic ISSN: 1476-9255
DOI: https://doi.org/10.1186/s12950-015-0068-1

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