Published in:
Open Access
01-12-2015 | Research
MicroRNA-181b regulates endotoxin tolerance by targeting IL-6 in macrophage RAW264.7 cells
Authors:
Wenjun Zhang, Xiaojun Shen, Luyang Xie, Maoping Chu, Yanmei Ma
Published in:
Journal of Inflammation
|
Issue 1/2015
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Abstract
Interleukin 6 (IL-6) is a major pro-inflammatory cytokine and dysregulation of IL-6 is relevant to many inflammatory diseases. Endotoxin induced tolerance of IL-6 is an important mechanism to avoid the excessive immune reaction. But to date, the molecular mechanisms of endotoxin tolerance of IL-6 remain unclear. Here we reported that IL-6 secretion and microRNA-181b (miR-181b) expression were inversely correlated following LPS stimulation. We also demonstrated that miR-181b targeting the 3′-UTR of IL-6 transcripts and up-regulation of miR-181b was associated with NF-kB. We further demonstrated that up-regulation of miR-181b in response to LPS was required for inducing IL-6 tolerance in macrophage. Our results suggested that the post-transcriptional control mediated by miR-181b could be involved in fine tuning the critical level of IL-6 expression in endotoxin tolerance.