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Molecular Cancer
Published in: Molecular Cancer 1/2017

Open Access 01-12-2017 | Research

Phospholipid Phosphatase 4 promotes proliferation and tumorigenesis, and activates Ca2+-permeable Cationic Channel in lung carcinoma cells

Authors: Xin Zhang, Lan Zhang, Bihua Lin, Xingxing Chai, Ronggang Li, Yuehua Liao, Xinghui Deng, Qiongru Liu, Wenli Yang, Yubo Cai, Wei Zhou, Zhichao Lin, Wenhai Huang, Meigong Zhong, Fangyong Lei, Jinhua Wu, Shuaishuai Yu, Xiaoping Li, Shangren Li, Yueyue Li, Jincheng Zeng, Wansheng Long, Dong Ren, Yanming Huang

Published in: Molecular Cancer | Issue 1/2017

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Abstract

Background

Phospholipid phosphatase 4 (PPAPDC1A or PLPP4) has been demonstrated to be involved in the malignant process of many cancers. The purpose of this study was to investigate the clinical significance and biological roles of PLPP4 in lung carcinoma.

Methods

PLPP4 expression was examined in 8 paired lung carcinoma tissues by real-time PCR and in 265 lung carcinoma tissues by immunohistochemistry (IHC). Statistical analysis was performed to evaluate the clinical correlation between PLPP4 expression and clinicopathological features and survival in lung carcinoma patients. In vitro and in vivo assays were performed to assess the biological roles of PLPP4 in lung carcinoma. Fluorescence-activated cell sorting, Western blotting and luciferase assays were used to identify the underlying pathway through which PLPP4 silencing mediates biological roles in lung carcinoma.

Results

PLPP4 is differentially elevated in lung adenocarcinoma (ADC) and lung squamous cell carcinoma (SQC) tissues. Statistical analysis demonstrated that high expression of PLPP4 significantly and positively correlated with clinicopathological features, including pathological grade, T category and stage, and poor overall and progression-free survival in lung carcinoma patients. Silencing PLPP4 inhibits proliferation and cell cycle progression in vitro and tumorigenesis in vivo in lung carcinoma cells. Our results further reveal that PLPP4 silencing inhibits Ca2+-permeable cationic channel, suggesting that downregulation of PLPP4 inhibits proliferation and tumorigenesis in lung carcinoma cells via reducing the influx of intracellular Ca2+.

Conclusion

Our results indicate that PLPP4 may hold promise as a novel marker for the diagnosis of lung carcinoma and as a potential therapeutic target to facilitate the development of novel treatment for lung carcinoma.
Appendix
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Metadata
Title
Phospholipid Phosphatase 4 promotes proliferation and tumorigenesis, and activates Ca2+-permeable Cationic Channel in lung carcinoma cells
Authors
Xin Zhang
Lan Zhang
Bihua Lin
Xingxing Chai
Ronggang Li
Yuehua Liao
Xinghui Deng
Qiongru Liu
Wenli Yang
Yubo Cai
Wei Zhou
Zhichao Lin
Wenhai Huang
Meigong Zhong
Fangyong Lei
Jinhua Wu
Shuaishuai Yu
Xiaoping Li
Shangren Li
Yueyue Li
Jincheng Zeng
Wansheng Long
Dong Ren
Yanming Huang
Publication date
01-12-2017
Publisher
BioMed Central
Published in
Molecular Cancer / Issue 1/2017
Electronic ISSN: 1476-4598
DOI
https://doi.org/10.1186/s12943-017-0717-5

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