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Malaria Journal
Published in: Malaria Journal 1/2016

Open Access 01-12-2016 | Research

Plasma levels of eight different mediators and their potential as biomarkers of various clinical malaria conditions in African children

Authors: Rachida Tahar, Catarina Albergaria, Neil Zeghidour, Vincent Foumane Ngane, Leonardo K. Basco, Christian Roussilhon

Published in: Malaria Journal | Issue 1/2016

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Abstract

Background

Plasmodium falciparum infection can lead to several clinical manifestations ranging from asymptomatic infections (AM) and uncomplicated malaria (UM) to potentially fatal severe malaria (SM), including cerebral malaria (CM). Factors implicated in the progression towards severe disease are not fully understood.

Methods

In the present study, an enzyme-linked immunosorbent assay (ELISA) method was used to investigate the plasma content of several biomarkers of the immune response, namely Neopterin, sCD163, suPAR, Pentraxin 3 (PTX3), sCD14, Fractalkine (CX3CL1), sTREM-1 and MIG (CXCL9), in patients with distinct clinical manifestations of malaria. The goal of this study was to determine the relative involvement of these inflammatory mediators in the pathogenesis of malaria and test their relevance as biomarkers of disease severity.

Results

ROC curve analysis show that children with AM were characterized by high levels of Fractalkine and sCD163 whereas children with UM were distinguishable by the presence of PTX3 in their plasma. Furthermore, principal component analysis indicated that the combination of Fractalkine, MIG, and Neopterin was the best predictor of AM condition, while suPAR, PTX3 and sTREM-1 combination was the best indicator of UM when compared to AM. The association of Neopterin, suPAR and Fractalkine was strongly predictive of SM or CM compared to UM.

Conclusions

The results indicate that the simultaneous evaluation of these bioactive molecules as quantifiable blood parameters may be helpful to get a better insight into the clinical syndromes in children with malaria.
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Metadata
Title
Plasma levels of eight different mediators and their potential as biomarkers of various clinical malaria conditions in African children
Authors
Rachida Tahar
Catarina Albergaria
Neil Zeghidour
Vincent Foumane Ngane
Leonardo K. Basco
Christian Roussilhon
Publication date
01-12-2016
Publisher
BioMed Central
Published in
Malaria Journal / Issue 1/2016
Electronic ISSN: 1475-2875
DOI
https://doi.org/10.1186/s12936-016-1378-3

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